LOCUS       sequence-236949-       11787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows fluorescent (mVENUS; mCherry) co-localization of two proteins
            in Agrobacterium- transformed plant cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11787)
  TITLE     Feher Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDOE20 mC VN
FEATURES             Location/Qualifiers
     source          1..11787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     promoter        complement(935..1315)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             1989..2696
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MMSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2402..2421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2729..3436
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4128..4182
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(4184..4186,4187..4189,4190..4900)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4229..4250)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4490..4509)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4837..4858
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4986..5238
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5289..5306)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5289..5305)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5298..5320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5508..5532
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6833..7462
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7891..8964
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9030..9224
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9460..9482)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9568..9708
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9623..9642
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9723..9740)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9894..10482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9974..9993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10569..11363)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcactctg
      421 actccttctc gaaggtttga gtaccttcag gacatcctct tgaaacatta gattcaactt
      481 caattggagc caactgcaaa agttcttgct tagacctaat tgccatctca cacaaatgtt
      541 gaagagttct tgatcctcca gaaacagtga tactaactcc cctgaaccta atagaataag
      601 tacatccgat ttgatcgaac ccaaagaatc ttgatgcagc atagttgact gaatctccag
      661 tccaagatcc aagcactcta tgcaatgatg cttcagttct atcgtatctg agatacctct
      721 taaacacaac ttttccgaaa ccccaagatt ccttgaaacc aagtggatta tcttgatttg
      781 agttcgtctc atcgttatgc aatctccact cagtccaaga tggactttca tcaggaagtt
      841 tgaaaggaga agttgtacct cctgatcctc catcccatct ttcagagtta gcttgttctc
      901 tagcatcatt tccttgtata gctcgttcca ttcgcgattt ggtgtatcga gattggttat
      961 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc
     1021 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt
     1081 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat
     1141 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc
     1201 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag
     1261 aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt gaaaaatctc
     1321 agaattcttg tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac
     1381 gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt
     1441 agccaaaaac aactttgcgt gtaaacaacg ctcaatacac ctgtcatttt attattagct
     1501 attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct
     1561 tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa
     1621 atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt
     1681 attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg
     1741 tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat
     1801 cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt
     1861 gtcgaataat tactcttcga tttgtgattt ctaactagat ctggtgttag tttctagttt
     1921 gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacagggaga ggacacgctc
     1981 gagccaccat gatgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     2041 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     2101 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     2161 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     2221 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     2281 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     2341 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2401 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2461 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2521 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2581 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2641 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtcta
     2701 gacccggggg ttaattaacc ctagagtcct gctttaatga gatatgcgag acgcctatga
     2761 tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga gcatgtgtag
     2821 ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg ttactatcgt
     2881 atttttatga ataatattct ccgttcaatt tactgattgt accctactac ttatatgtac
     2941 aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat ctatgataga
     3001 gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa aaaaagcggc
     3061 agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc aaaagtgccc
     3121 caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact gaagggaact
     3181 ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg gccgtccgct
     3241 ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg taaccgactt
     3301 gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat gaagtgcagg
     3361 tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt tgcgacaaca
     3421 tgtcgaggct cagcaggacc tgctccaaga gtatggttgt cgacgagtca gtaataaacg
     3481 gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa agcacaaata
     3541 cttttcctca acctaaaaat aaggcaatta gccaaaaaca actttgcgtg taaacaacgc
     3601 tcaatacacc tgtcatttta ttattagcta ttgcttcacc gccttagctt tctcgtgacc
     3661 tagtcgtcct cgtcttttct tcttcttctt ctataaaaca atacccaaag agctcttctt
     3721 cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa ttctctctac
     3781 cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt
     3841 tcgatcccaa tttcgtatat gttctttggt ttagattctg ttaatcttag atcgaagacg
     3901 attttctggg tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc
     3961 atccgatttg ttcaaataat ttgagttttg tcgaataatt actcttcgat ttgtgatttc
     4021 taactagatc tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt
     4081 tttctgatta acagggacac gcctcaggta cagagcatat ttttacaaca attaccaaca
     4141 acaacaaaca acaaacaaca ttacaattac atttacaatt acaatggtga gcaagggcga
     4201 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4261 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4321 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
     4381 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4441 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4501 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4561 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4621 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     4681 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     4741 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     4801 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4861 cgccgccggg atcactctcg gcatggacga gctgtacaag tcaggtgcag gttctggtgg
     4921 aggtagcggg atggggtccc tacgtagtca cgtgacgtcc ggatgagcga tcgccattta
     4981 aataggatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     5041 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     5101 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     5161 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     5221 catctatgtt actagatcgt taacgatatg tcgaggctca gcaggacctg caggcatgca
     5281 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5341 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5401 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc
     5461 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     5521 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     5581 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     5641 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     5701 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     5761 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     5821 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     5881 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     5941 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     6001 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     6061 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     6121 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     6181 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     6241 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     6301 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     6361 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     6421 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     6481 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     6541 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     6601 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     6661 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     6721 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     6781 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     6841 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     6901 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     6961 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     7021 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     7081 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     7141 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     7201 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     7261 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     7321 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     7381 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     7441 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     7501 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     7561 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     7621 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     7681 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     7741 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     7801 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg
     7861 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
     7921 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     7981 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     8041 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     8101 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     8161 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     8221 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     8281 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     8341 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     8401 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     8461 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     8521 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     8581 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     8641 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     8701 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     8761 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     8821 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     8881 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     8941 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     9001 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
     9061 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     9121 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     9181 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     9241 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     9301 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     9361 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     9421 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     9481 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     9541 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     9601 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9661 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     9721 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9781 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10321 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    10561 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
    10621 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
    10681 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
    10741 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
    10801 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
    10861 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
    10921 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
    10981 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
    11041 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
    11101 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
    11161 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
    11221 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
    11281 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
    11341 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
    11401 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
    11461 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
    11521 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
    11581 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
    11641 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
    11701 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    11761 ccggtcgggg agctgttggc tggctgg
//
