LOCUS       sequence-236959-       10145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentivirus expressing an arbitrary shRNA and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pTRE:shRNA A-pGK:rtTA-Cre
FEATURES             Location/Qualifiers
     source          1..10145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2101..2145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2443..2560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2671..2985
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2679..2697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2715..2733
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2750..2768
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2786..2804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2822..2840
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2857..2875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2893..2911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2958..2982
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3037..3039,3040..3042,3043..3756)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3343..3362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3690..3711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           3800..3921
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3949..3963
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3991..4117
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(4166..4189)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             join(4573..5190,5191..5196,5197..5232,5233..5235,5236..5271,
                     5272..5274,5275..5310,5311..5313)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYRHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLP"
     CDS             5332..5385
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5392..5412
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5413..6441
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    6494..7082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6547..6567)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6965..6976)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7085..7101)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7086..7102)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7611..7791
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7853..8441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7933..7952)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9151..9942,9943..10011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9774..9793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10012..10116)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag taccgggccc gggtcgacta gtgatcagtg tgagggagtg
     2641 taaagctggt ttcgacggtt tcgatatgtc gagtttactc cctatcagtg atagagaacg
     2701 tatgtcgagt ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt
     2761 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     2821 ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta
     2881 tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg
     2941 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag aatacgtaag
     3001 tcactaactc gaacgctagc tgtgcgatcg tttaccatgg tgagcaaggg cgaggagctg
     3061 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3121 agcgtgtctg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3241 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3481 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggcgaa cttcaagatc
     3541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     3661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3721 gggatcactc tcggcatgga cgagctgtac aagtaatgac gtacggctaa gcctcgacta
     3781 gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
     3841 atcttggaaa cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag
     3901 gtatattgct gttgacagtg agcgcccact acaagtacat gtgtaatagt gaagccacag
     3961 atgtattaca catgtacttg tagtggatgc ctactgcctc ggaattcaag gggctacttt
     4021 aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga tacattttta
     4081 caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattctacc gggtagggga
     4141 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     4201 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     4261 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     4321 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     4381 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     4441 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     4501 gggcgggctc aggggcgggc tcaggggcgg ggcggccgcg acccgacatt agcgctacag
     4561 cttaagcgga ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta
     4621 ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt
     4681 gagcagccta ccctgtaccg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca
     4741 atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa
     4801 gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg
     4861 gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag
     4921 ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc
     4981 gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag
     5041 gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg
     5101 ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc
     5161 ctggagaaac agctaaagtg cgaaagcggc gggccgaccg atgcccttga cgattttgac
     5221 ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac
     5281 gctcttgacg attttgacct tgacatgctc ccctggggaa gtggagctag ggagggcaga
     5341 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctgggat gcccaagaag
     5401 aagaggaagg tgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat
     5461 gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt
     5521 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
     5581 ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat
     5641 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
     5701 cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg
     5761 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
     5821 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat
     5881 atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
     5941 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
     6001 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
     6061 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
     6121 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
     6181 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
     6241 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
     6301 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
     6361 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
     6421 ctgctggaag atggcgatta gaaacgttgt tgtttggggt tgaattactc ttctagaccg
     6481 cggcgcgccc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6541 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6601 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6661 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6721 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6781 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6841 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     6901 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6961 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7021 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7081 gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca
     7141 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7201 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7261 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7321 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     7381 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     7441 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     7501 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     7561 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     7621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatgtgag
     7801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8041 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8281 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8461 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8521 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9121 ttactgtcat gccatccgta agatgctttt ctaccaatgc ttaatcagtg aggcacctat
     9181 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9241 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9301 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9361 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9421 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9481 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9541 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9601 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9661 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9721 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9781 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9841 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9901 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9961 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10021 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10081 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10141 tgacg
//
