LOCUS       sequence-236960-        4922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with ymYPET with CaUra3
            selection. *An updated version of this plasmid is available: #168056
            pFA6a-link-ymYPET-URA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-ymYPET-CaURA3
FEATURES             Location/Qualifiers
     source          1..4922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        119..137
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     119..136
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      966..1153
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1021..1045)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1228..1571
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2390..2587
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2694..2713)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2695..2713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2800..2817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2971..3559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3051..3070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3730..4521,4522..4590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4353..4372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4591..4695)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4763..4781
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4819..4841)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca
       61 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
      121 ttaggtgaca ctatagaacg cggccgccag ctgaagcttc gtacgctgca ggtcgacgga
      181 tcggtgacgg tgctggttta attaaatggt ctccaagggc gaagaattgt tcaccggtgt
      241 ggtcccaatc ttggtcgagt tggacggtga cgtcaacggt cacaagttct ctgtttccgg
      301 tgaaggtgaa ggagacgcca cctacggtaa attgaccttg aagttgttgt gcaccaccgg
      361 caagctgcca gttccatggc caacattggt caccacttta ggttatggtt tacaatgttt
      421 tgctagatat ccagatcata tgaaacaaca tgatttcttc aaatctgcta tgccagaagg
      481 ttatgttcaa gaaagaacta tatttttcaa agatgatggt aattataaaa ctagagctga
      541 agttaaattt gaaggagata ctttagttaa tagaattgaa ttgaaaggta ttgattttaa
      601 agaagatggt aatattttag gtcataaatt agaatataat tataattctc ataatgttta
      661 tattacagct gataaacaaa agaatggtat taaggctaat tttaaaatta gacataatat
      721 tgaagatggt ggtgttcaat tagctgatca ttatcaacaa aatactccta ttggtgacgg
      781 tccagtttta ttacctgata atcattattt gtcatatcaa tctaaattgt caaaagatcc
      841 aaatgaaaaa agagatcata tggttttgtt agaatttttg actgcagctg gtattacttt
      901 aggtatggat gaattgtata aataagcggc cgcctcgagg tcgacggcgc gccacttcta
      961 aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     1021 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     1081 actctttcct gtaggtcagg ttgctttctc aggtatagta tgaggtcgct cttattgacc
     1141 acacctctac cggcagatcc gctagggata acagggtaat atagatctgt ttagcttgcc
     1201 tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt
     1261 cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat
     1321 ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat
     1381 tacggctcct cgctgcggac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat
     1441 tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc
     1501 ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa
     1561 cataaacaac catgacagtc aacactaaga cctatagtga gagagcagaa actcatgcct
     1621 caccagtagc acaacgatta tttcgattaa tggaactgaa gaaaaccaat ttatgtgcat
     1681 caattgatgt tgataccact aaggaattcc ttgaattaat tgataaattg ggtccttatg
     1741 tatgcttaat caagactcat attgatataa tcaatgattt ttcctatgaa tccactattg
     1801 aaccattatt agaactttca cgtaaacatc aatttatgat ttttgaagat agaaaatttg
     1861 ctgatattgg taataccgtg aagaaacaat atattggtgg agtttataaa attagtagtt
     1921 gggcagatat tactaatgct catggtgtca ctgggaatgg agtagttgaa ggattaaaac
     1981 agggagctaa agaaaccacc accaaccaag agccaagagg gttattgatg ttagctgaat
     2041 tatcatcagt gggatcatta gcatatggag aatattctca aaaaactgtt gaaattgcta
     2101 aatccgataa ggaatttgtt attggattta ttgcccaacg tgatatgggt ggacaagaag
     2161 aaggatttga ttggcttatt atgacacctg gagttggatt agatgataaa ggtgatggat
     2221 taggacaaca atatagaact gttgatgaag ttgttagcac tggaactgat attatcattg
     2281 ttggtagagg attgtttggt aaaggaagag atccagatat tgaaggtaaa aggtatagag
     2341 atgctggttg gaatgcttat ttgaaaaaga ctggccaatt ataatcagta ctgacaataa
     2401 aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct
     2461 attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga
     2521 tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc
     2581 tatactgctg tcgattcgat actaacgccg ccatccagtg tcgaaaacga gctcgaattc
     2641 atcgatgata tcagatccac tagtggccta tgcggccgcg gatctgccgg tctccctata
     2701 gtgagtcgta ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc
     2761 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2821 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2881 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3601 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3661 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3721 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3781 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3841 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3901 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3961 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4021 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4081 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4141 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4201 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4261 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4321 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4381 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4441 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4501 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4561 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4621 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4681 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4741 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     4801 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     4861 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4921 tc
//
