LOCUS       sequence-236963-        5633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            tdTomato with CaUra3 selection. *An updated version of this plasmid 
            is available: #168054 pFA6a-link-ytdTomato-URA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-ytdTomato-CaURA3
FEATURES             Location/Qualifiers
     source          1..5633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        69..87
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     69..86
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1627..1814
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1682..1706)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1889..2232
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3051..3248
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3355..3374)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3356..3374)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3461..3478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3632..4220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3712..3731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4391..5182,5183..5251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5014..5033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5252..5356)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5424..5442
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5480..5502)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5602..5621
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
       61 cacatacgat ttaggtgaca ctatagaacg cggccgccag ctgaagcttc gtacgctgca
      121 ggtcgacgga tcggtgacgg tgctggttta attaaatggt tagtaaaggt gaggaagtga
      181 ttaaggaatt catgaggttt aaagttagaa tggaaggttc tatgaatggt catgaatttg
      241 aaattgaagg tgaaggtgaa ggtagaccat atgaaggtac acaaactgct aaattgaaag
      301 ttacaaaagg tggtccatta ccatttgcat gggatatttt atctccacag tttatgtatg
      361 gtagtaaagc atatgttaaa catccagctg atattccaga ttataagaaa ttaagttttc
      421 ctgaaggttt taaatgggaa agagtaatga attttgaaga tggtggttta gtaacagtta
      481 ctcaagattc ttctttacaa gatggtacat taatatataa agttaaaatg agaggtacta
      541 attttccacc tgatggtcca gttatgcaga agaaaacaat gggttgggaa gcctcaactg
      601 aaagattgta ccctagagat ggagttttga aaggtgaaat tcatcaagct cttaagttaa
      661 aagatggagg tcactatttg gttgagttta aaactatata catggctaaa aaacctgttc
      721 aattgccagg ttattattat gttgatacta aattagatat tacttcacat aatgaagatt
      781 atactattgt tgaacaatat gaaagatctg aaggtagaca tcatttgttt ttgggtcatg
      841 gtactggtag tactggttct ggttcttcag gtactgcttc tagtgaagat aataatatgg
      901 ctgtaatcaa agagttcatg aggtttaaag ttagaatgga aggttctatg aatggtcatg
      961 agtttgaaat cgaaggtgaa ggagaaggta gaccatatga aggtactcaa actgctaaat
     1021 tgaaagttac taaaggtggt ccattgccat ttgcttggga tatattgtca cctcagttta
     1081 tgtatggttc taaagcatat gttaaacatc cagctgatat tcctgattat aaaaaattat
     1141 catttcctga aggttttaaa tgggaaagag ttatgaattt tgaagatggt ggtttagtta
     1201 ctgtaactca agattcttca ttgcaagatg gtacattaat atataaagtt aaaatgagag
     1261 gtactaattt tccacctgat ggtccagtta tgcagaaaaa gactatgggt tgggaagcat
     1321 ctactgaaag attatatcct agagatggtg ttttaaaagg tgaaattcac caagccttaa
     1381 aattgaaaga tggtggtcat tatttggttg aattcaaaac aatctacatg gctaaaaaac
     1441 cagttcagct gcccggatac tattatgtcg atactaaact ggatattact tcacacaatg
     1501 aagactacac aattgttgaa cagtatgaaa gatcagaggg tagacatcac ttgtttttat
     1561 acggtatgga tgaattatat aaataagcgg ccgcctcgag gtcgacggcg cgccacttct
     1621 aaataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
     1681 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt
     1741 aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac
     1801 cacacctcta ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc
     1861 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
     1921 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
     1981 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
     2041 ttacggctcc tcgctgcgga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
     2101 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
     2161 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
     2221 acataaacaa ccatgacagt caacactaag acctatagtg agagagcaga aactcatgcc
     2281 tcaccagtag cacaacgatt atttcgatta atggaactga agaaaaccaa tttatgtgca
     2341 tcaattgatg ttgataccac taaggaattc cttgaattaa ttgataaatt gggtccttat
     2401 gtatgcttaa tcaagactca tattgatata atcaatgatt tttcctatga atccactatt
     2461 gaaccattat tagaactttc acgtaaacat caatttatga tttttgaaga tagaaaattt
     2521 gctgatattg gtaataccgt gaagaaacaa tatattggtg gagtttataa aattagtagt
     2581 tgggcagata ttactaatgc tcatggtgtc actgggaatg gagtagttga aggattaaaa
     2641 cagggagcta aagaaaccac caccaaccaa gagccaagag ggttattgat gttagctgaa
     2701 ttatcatcag tgggatcatt agcatatgga gaatattctc aaaaaactgt tgaaattgct
     2761 aaatccgata aggaatttgt tattggattt attgcccaac gtgatatggg tggacaagaa
     2821 gaaggatttg attggcttat tatgacacct ggagttggat tagatgataa aggtgatgga
     2881 ttaggacaac aatatagaac tgttgatgaa gttgttagca ctggaactga tattatcatt
     2941 gttggtagag gattgtttgg taaaggaaga gatccagata ttgaaggtaa aaggtataga
     3001 gatgctggtt ggaatgctta tttgaaaaag actggccaat tataatcagt actgacaata
     3061 aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc
     3121 tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag
     3181 atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg
     3241 ctatactgct gtcgattcga tactaacgcc gccatccagt gtcgaaaacg agctcgaatt
     3301 catcgatgat atcagatcca ctagtggcct atgcggccgc ggatctgccg gtctccctat
     3361 agtgagtcgt attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg
     3421 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3481 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3541 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3601 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3661 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3721 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3781 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3841 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3901 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3961 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4021 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4081 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4141 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4201 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4261 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4321 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4381 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4441 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4501 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4561 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4621 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4681 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4741 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4801 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4861 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4921 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4981 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5041 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5101 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5161 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5221 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5281 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5341 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     5401 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     5461 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     5521 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     5581 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg cac
//
