LOCUS       sequence-236968-       10099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multipartite assembly obtained by combining FB parts FB026+FB003 
            into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg 
            resistance +YFP) according to FungalBraid modular DNA assembly for 
            ATMT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB027
FEATURES             Location/Qualifiers
     source          1..10099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(316..567)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(358..379)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(514..1026,1027..1029,1030..1032))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     707..726
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     966..987
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2136..3161
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2136..3161
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    3461..3485
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4786..5415
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5844..6917
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6983..7177
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7413..7435)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7521..7661
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7576..7595
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7676..7693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7847..8435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7927..7946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8523..9314)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacataac ggatccggtc tcaggagagc gcatgtctca
       61 gacggtcgat gcattgcaga tgagctgtat ctggaagagg taaacccgaa acgcgtttta
      121 ttcttgttga catggagcta ttaaatcact agaaggcact ctttgctgct tggacaaatg
      181 aacgtatctt atcgagatcc tgaacaccat ttgtctcaac tccggagctg acatcgacac
      241 caacgatctt atatccagat tcgtcaagct gtttgatgat ttcagtaacg ttaagtaagc
      301 tcagctcatg actgacttgt agagctcgtc catgccgaga gtgatcccgg cggcggtcac
      361 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      421 gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      481 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      541 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      601 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      661 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      721 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      781 gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cacttcaggc cgtagccgaa
      841 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      901 ggtcagcttg ccgtaggtgg cgtcgccctc gccctcgccg gacacgctga acttgtggcc
      961 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcttcacc
     1021 cttgctcacc attttaaagg ttcttggatg ggaagatgaa tatactgaag atgggaaaag
     1081 aaagagaaaa gaaaagagca gctggtgggg agagcaggaa aatatggcaa caaatgttgg
     1141 actgacgcaa cgaccttgtc aaccccgccg acacaccggg cggacagacg gggcaaagct
     1201 gcctaccagg gactgaggga cctcagcagg tcgagtgcag agcaccggat gggtcgactg
     1261 ccagcttgtg ttcccggtct gcgccgctgg ccagctcctg agcggccttt ccggtttcat
     1321 acaccgggca aagcaggaga ggcacgatat ttggacgccc tacagatgcc ggatgggcca
     1381 attagggagc ttacgcgccg ggtactcgct ctacctactt cggagaaggt actatctcgt
     1441 gaatctttta ccagatcgga agcaattgga cttctgtacc taggttaatg gcatgctatt
     1501 tcgccgacgg ctatacaccc ctggcttcac attctccttc gcttactgcc ggtgattcga
     1561 tgaagctcca tattctccga tgatgcaata gattcttggt caacgagggg cacaccagcc
     1621 tttccacttc ggggcggagg ggcggccggt cccggattaa taatcatcca ctgcacctca
     1681 gagccgccag agctgtctgg cgcagtggcg cttattactc agcccttctc tctgcgtccg
     1741 tccgtctgtc cgcatgcgcc actccgtcag gagaactgat attgaaggag cattttttgg
     1801 gcttggctgg agctagtgga ggtcaacaat gaatgcctat tttggtttag tcgtccaggc
     1861 ggtgagcaca aaatttgtgt cgtttgacaa gatggttcat ttaggcaact ggtcagatca
     1921 gccccacttg tagcagtagc ggcggcgctc gaagtgtgac tcttattagc agacaggaac
     1981 gaggacatta ttatcatctg ctgcttggtg cacgataact tggtgcgttt gtcaagcaag
     2041 gtaagtggac gacccggtca taccttctta agttcgccct tcctcccttt atttcagatt
     2101 caatctgact tacctattct acccaagcat ccaaaatgaa aaagcctgaa ctcaccgcga
     2161 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt gtccgacctg atgcagctct
     2221 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     2281 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     2341 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     2401 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     2461 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     2521 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     2581 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     2641 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     2701 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     2761 gccgcataac agcggtcatt gactggagcg aggcaatgtt cggggattcc caatacgagg
     2821 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     2881 tcgagcggag gcatccggag cttgcaggat cgccacgact ccgggcgtat atgctccgca
     2941 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     3001 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     3061 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     3121 gaaaccgacg ccccagcact cgtccgaggg caaaggaatg agcttcatca ttccaccatc
     3181 attccactca acattcaggc tcctctgcgc acgtaaagtg ccaaaggcaa taccctgctc
     3241 ggtggaatgc cgccgggctt gtcgatttta cgcacatatg cgcattcttg acttgaagcg
     3301 gaggagttct tcgttgcggg ttacagtgtt ttaataaaag aatggtcaaa tcaaactgct
     3361 agatatacct gtcagacact ctagttgttg acccctatac tcttaataca tcagacagta
     3421 catgcatgtt gcatgatgac gctgtcatga gaccggatcc tgacaggata tattggcggg
     3481 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     3541 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     3601 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     3661 cgtcatctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     3721 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     3781 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     3841 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     3901 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     3961 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     4021 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     4081 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     4141 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     4201 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     4261 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     4321 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     4381 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     4441 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     4501 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     4561 gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg
     4621 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     4681 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     4741 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     4801 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     4861 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     4921 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     4981 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     5041 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     5101 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     5161 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     5221 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     5281 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     5341 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     5401 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     5461 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     5521 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     5581 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     5641 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     5701 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     5761 acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga
     5821 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     5881 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     5941 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     6001 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     6061 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     6121 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     6181 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     6241 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     6301 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     6361 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     6421 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     6481 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     6541 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     6601 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     6661 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     6721 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     6781 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     6841 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     6901 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     6961 aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     7021 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     7081 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     7141 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     7201 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     7261 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     7321 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     7381 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggtgacg
     7441 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7501 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     7561 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     7621 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     7681 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7741 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7801 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7861 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7921 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7981 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8041 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8101 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8161 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8221 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8281 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8341 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8401 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8461 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     8521 gattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     8581 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     8641 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     8701 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     8761 tttcgctcat caccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     8821 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
     8881 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
     8941 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
     9001 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
     9061 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
     9121 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
     9181 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
     9241 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
     9301 accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     9361 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     9421 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     9481 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     9541 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     9601 ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc atttctgtcc
     9661 tggctggaac agaacttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     9721 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     9781 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     9841 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     9901 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     9961 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
    10021 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
    10081 ggagctgttg gctggctgg
//
