LOCUS       sequence-236974-        2874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription plasmid for T7 transcription of KRAS WT 
            sequence targeted to the endosome. Linearize with SapI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Veatch JR, Jesernig BL, Kargl J, Fitzgibbon M, Lee SM, Baik C, 
            Martins R, Houghton AM, Riddell SR
  TITLE     Endogenous CD4(+) T Cells Recognize Neoantigens in Lung Cancer 
            Patients, Including Recurrent Oncogenic KRAS and ERBB2 (Her2) Driver
            Mutations.
  JOURNAL   Cancer Immunol Res. 2019 Jun;7(6):910-922. doi: 
            10.1158/2326-6066.CIR-18-0402. Epub 2019 May 1.
  PUBMED    31043415
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2326-6066.CIR-18-0402"; journalName: "Cancer Immunol Res"; 
            date: "2019-06"; volume: "7"; issue: "6"; pages: "910-922"
COMMENT     Sequence Label: pJV128
FEATURES             Location/Qualifiers
     source          1..2874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(136..153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(246..834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(326..345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1005..1796,1797..1865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1628..1647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1866..1970)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2271..2293
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2285..2302
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2286..2302
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2340..2359
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2340..2358
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgaagagcga ctactggcat gctgggcctc atgggccttc cgctcactgc ccgctttcca
       61 gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat agctgtttcc ttgcgtattg
      121 ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg taaagcctgg
      181 ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1141 ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1981 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2041 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2101 aagaatagac cgagataggg ttgagtggcc gctacagggc gctcccattc gccattcagg
     2161 ctgcgcaact gttgggaagg gcgtttcggt gcgggcctct tcgctattac gccagctggc
     2221 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     2281 acgttgtaaa acgacggcca gtgagcgcga cgattggcgg aaggccgtca aggccgcatt
     2341 aatacgactc actatagggt tttccaccat gcgcgtaacg gcaccccgga cactgcttct
     2401 ccttttgtgg ggagcggtcg ctctgaccga gacttgggcc ggatccatga cagaatataa
     2461 gctggtggtc gtcggtgcag gaggtgtcgg taagtctgcc ctcacgatcc agttgatcca
     2521 gaccggtatt gtaggcatcg tggcaggctt ggctgttctg gccgttgtcg ttatcggcgc
     2581 agttgtggcc gccgtcatgt gccggcggaa atcctccggc ggcaaaggcg gttcttactc
     2641 ccaggctgct tgttcagatt ctgcccaggg gagtgacgtt tccttgaccg cttaagctcg
     2701 ctttcttgct gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac
     2761 tgggggatat tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt
     2821 tcattgctgc tctagataaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//
