LOCUS       sequence-236999-        2335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Part. CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pC0.123
FEATURES             Location/Qualifiers
     source          1..2335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..47)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        115..219
                     /gene="bla"
                     /label=AmpR promoter
     CDS             441..1232
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1403..1991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1892..1911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2145..2162
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
       61 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      121 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      181 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
      241 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
      301 gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
      361 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
      421 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
      481 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
      541 acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg
      601 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
      661 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
      721 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
      781 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
      841 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
      901 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
      961 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     1021 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     1081 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     1141 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa
     1201 aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac
     1261 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1321 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1381 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1441 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1501 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1561 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1621 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1681 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1741 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1801 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1861 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1921 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1981 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2041 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2101 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2161 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2221 aatcactctg tggtctctgt ttctgttggg ccattgcatt gccactgatt ttccaacata
     2281 taaaaagaca agcccgaaca gtcgtccggg ctttttttct cgagcgctag agacc
//
