LOCUS       sequence-23703-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ38 (miR-1006 seed mutant perfect site)
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             201..242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             252..269
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(292..309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    311..445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(335..354)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     389..408
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(650..670)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgagttctat ctgaatttgt caagtaaaag gtcttcattt ttcgcaagaa gatgcacctg
       61 atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat
      121 tctaggagct cctatgaata agaaatccat tataactagt tgttctagag cggccgctcg
      181 agtctagagg gcccttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
      241 cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
      301 tgccttctaa gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
      361 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      421 cattataagc tgcaataaac aagttctaga gtcggtgggc ctcgggggcg ggtgcggggt
      481 cggcggggcc gccccgggtg gcttcggtcg gagccatggg gtcgtgcgct cctttcggtc
      541 gggcgctgcg ggtcgtgggg cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg
      601 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
      661 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
      721 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
      781 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
      841 atctgttgct gcgcggccag cnggaccgct cactcggcca tgcgcggcga ttcgcaaacg
      901 ccntcagt
//
