LOCUS       sequence-23704-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ39 (miR-1003 seed mutant perfect site)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             199..240
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             250..267
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(290..307)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    309..443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(333..352)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     387..406
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(648..668)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 agtttctatc tgaatttgtc aagtaaaagg tcttcatttt tcgcaagaag atgcacctga
       61 tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt
      121 ctaggagctc ctgtgaatat gtaaaagagt gaactagttg ttctagagcg gccgctcgag
      181 tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
      241 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
      301 ccttctaaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      361 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      421 ttataagctg caataaacaa gttctagagt cggtgggcct cgggggcggg tgcggggtcg
      481 gcggggccgc cccgggtggc ttcggtcgga gccatggggt cgtgcgctcc tttcggtcgg
      541 gcgctgcggg tcgtggggcg ggcgtcaggc accgggcttg cgggtcatgc accaggtgcg
      601 cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg
      661 gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc
      721 cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc
      781 gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat
      841 ctgttgctgc gcggccagcc gggaaaccgc tcaactcggc agccggcgac cgcacacgcc
      901 ntca
//
