LOCUS       sequence-23705-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ40 (let-7 seed mutant perfect site)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             203..244
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             254..271
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(294..311)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    313..447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(337..356)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     391..410
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(652..672)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cagaagtttc taatctgaat ttgtcaagta aaaggtcttc atttttcgca agaagatgca
       61 cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa
      121 taattctagg agctcactat acaacctaca agcacaacta gttgttctag agcggccgct
      181 cgagtctaga gggcccttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
      241 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
      301 tgtgccttct aagatccaga catgataaga tacattgatg agtttggaca aaccacaact
      361 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      421 accattataa gctgcaataa acaagttcta gagtcggtgg gcctcggggg cgggtgcggg
      481 gtcggcgggg ccgccccggg tggcttcggt cggagccatg gggtcgtgcg ctcctttcgg
      541 tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg
      601 tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga
      661 aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg
      721 cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga
      781 cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcttc
      841 catctgtgct gcgcggcagc ggacgtcatc gcngcggcat cnaacgcctc atcggg
//
