LOCUS       sequence-237064-        9217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP linked to human Tau.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9217)
  TITLE     Bacterial expression plasmids and lentivirus expression vectors for 
            tau and alpha-synuclein
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLJM1 EGFP-Tau
FEATURES             Location/Qualifiers
     source          1..9217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        47..273
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             274..454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    501..626
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1119..1352
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1537..1581
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1879..1899
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2188..2391
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2342..2362
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2435..2444
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2441..2443,2444..2446,2447..3157)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSNQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2486..2507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2747..2766)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3094..3115
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4357..4474
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4523..5033
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4952..4970
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5055..5654
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5055..5074)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5551..5571
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5782..6015
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6087..6208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6124..6143)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6178..6197
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6248..6383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6403..6422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6404..6422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6432..6449)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6432..6448)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6441..6463)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6590..7045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6677..6696)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6887..6908
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7071..7175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7176..7244,7245..8036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7394..7413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8207..8795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8696..8715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8949..8966
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9083..9104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9119..9124,9125..9142,9143..9149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9157..9173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9162..9184
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9181..9197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9181..9197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(9216..9217,1..19)
                     /label=T3
                     /note="T3 promoter, forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
       61 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      121 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      181 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      241 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      421 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      481 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      541 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      601 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      661 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      721 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      781 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      841 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      901 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      961 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1021 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1081 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1141 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1201 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1261 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1321 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1381 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1441 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1501 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1561 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1621 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1681 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1741 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1801 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     1861 gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     1921 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     1981 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     2041 tttaaaatgg actatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2101 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2161 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2221 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2281 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2341 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     2401 aaccgtcaga tccgctagcg ctaccggtat ggtcgccacc atggtgagca agggcgagga
     2461 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2521 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2581 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2641 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2701 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2761 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2821 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2881 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2941 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3001 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca accagtccgc
     3061 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3121 cgccgggatc actctcggca tggacgagct gtacaaggga tccgctgagc cccgccagga
     3181 gttcgaagtg atggaagatc acgctgggac gtacgggttg ggggacagga aagatcaggg
     3241 gggctacacc atgcaccaag accaagaggg tgacacggac gctggcctga aagctgaaga
     3301 agcaggcatt ggagacaccc ccagcctgga agacgaagct gctggtcacg tgacccaagc
     3361 tcgcatggtc agtaaaagca aagacgggac tggaagcgat gacaaaaaag ccaagggggc
     3421 tgatggtaaa acgaagatcg ccacaccgcg gggagcagcc cctccaggcc agaagggcca
     3481 ggccaacgcc accaggattc cagcaaaaac cccgcccgct ccaaagacac cacccagctc
     3541 tggtgaacct ccaaaatcag gggatcgcag cggctacagc agccccggct ccccaggcac
     3601 tcccggcagc cgctcccgca ccccgtccct tccaacccca cccacccggg agcccaagaa
     3661 ggtggcagtg gtccgtactc cacccaagtc gccgtcttcc gccaagagcc gcctgcagac
     3721 agcccccgtg cccatgccag acctgaagaa tgtcaagtcc aagatcggct ccactgagaa
     3781 cctgaagcac cagccgggag gcgggaaggt gcagataatt aataagaagc tggatcttag
     3841 caacgtccag tccaagtgtg gctcaaagga taatatcaaa cacgtcccgg gaggcggcag
     3901 tgtgcaaata gtctacaaac cagttgacct gagcaaggtg acctccaagt gtggctcatt
     3961 aggcaacatc catcataaac caggaggtgg ccaggtggaa gtaaaatctg agaagcttga
     4021 cttcaaggac agagtccagt cgaagattgg gtccctggac aatatcaccc acgtccctgg
     4081 cggaggaaat aaaaagattg aaacccacaa gctgaccttc cgcgagaacg ccaaagccaa
     4141 gacagaccac ggggcggaga tcgtgtacaa gtcgccagtg gtgtctgggg acacgtctcc
     4201 acggcatctc agcaatgtct cctccaccgg cagcatcgac atggtagact cgccccagct
     4261 cgccacgcta gctgacgagg tgtctgcctc cctggccaag cagggtttgt gataagaatt
     4321 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     4381 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     4441 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     4501 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
     4561 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     4621 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     4681 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     4741 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     4801 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     4861 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     4921 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     4981 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     5041 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     5101 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     5161 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     5221 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     5281 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     5341 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     5401 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     5461 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5521 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5581 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     5641 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     5701 cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     5761 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     5821 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5881 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5941 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6001 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6061 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6121 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6181 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6301 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6361 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     6421 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6481 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6541 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6601 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6661 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6721 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6781 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6841 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6901 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6961 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7021 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7081 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7141 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7201 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7261 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7321 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7381 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7441 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7501 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7561 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7621 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7681 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7741 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7801 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7861 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7921 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7981 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8041 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8101 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8341 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8581 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8821 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8881 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8941 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9001 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9061 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9181 caggaaacag ctatgaccat gattacgcca agcgcgc
//
