LOCUS       sequence-23707-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ45 (miR-1003 5' splice site mut)
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             412..453
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             463..480
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(503..520)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    522..656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(546..565)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     600..619
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tctctgttaa gccgatagcg tgcggaaatc gaaaagaccg agcgtgaacg actgcaacag
       61 caggaacgcg aggatgagat gcgcgaactg gccctcaagc tgcgcagaac gtgggcatct
      121 ggatgtggtt ggctctggcg gtcctctcac atttacatgt tcacaggcgc cgtgagctgc
      181 gtcacaaata tggaacgccc tcgagtggaa agctctcgga cagcgatgcc gaatctgtgg
      241 catcggaaag ccagctggca aagtcgtcga cgcgtcgcag tcgcagcaga tcggagagtc
      301 gtaggcggtc gccaccgccg gaaacacagc acagcagacg gagcagcagc cgaacggaaa
      361 ggaggagacg cacaaggacg gcggccgctc gagtctagag ggcccttcga aggtaagcct
      421 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
      481 tgagtttaaa cccgctgatc agcctcgact gtgccttcta agatccagac atgataagat
      541 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      601 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttctag
      661 agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt ggcttcggtc
      721 ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca
      781 ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc
      841 ggtgacggtg aagccgaacc gctcgtaaaa ngggangttg cgggcgcgaa gtctcaggaa
      901 gcggcacccg ggcgtcgccg ctccntcngg ga
//
