LOCUS       sequence-237071-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Study of protein insertion into nanodisks by means of changes in 
            tryptophan fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  TITLE     Bacterial expression plasmid for production of recombinant 
            MSP1D12W/2Y protein where 2 tryptophan residues are substituted with
            2 tyrosines
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSP1D12W/2Y
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             698..715
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(768..786)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      782..829
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctagaatant tttgtttaac tttaagaagg agatatacca tgggtcatca tcatcnncat
       61 natcacgatt atgatattcc tactactgag aatttgtatt ttcagggttc taccttcagt
      121 aaacttcgcg aacaactggg ccccgtgacg caggaattct acgacaacct ggaaaaagaa
      181 accgagggac tgcgtcagga aatgtccaaa gatttagaag aggtgaaggc caaggttcag
      241 ccatatctcg atgactttca gaaaaaatac caggaagaga tggaattata tcgtcaaaag
      301 gtggaaccgc tgcgtgcgga actgcaagag ggggcacgcc aaaaactcca tgagctccaa
      361 gagaagctca gcccattagg cgaagaaatg cgcgatcgcg cccgtgcaca tgttgatgca
      421 ctccggactc atttggcgcc gtattcggat gaacttcgcc agcgtttggc cgcacgtctc
      481 gaggcgctga aagaaaacgg gggtgcccgc ttggctgagt accacgcgaa agcgacagaa
      541 cacctgagca ccttgagcga aaaagcgaaa ccggcgctgg aagatctacg ccagggctta
      601 ttgcctgttc ttgagagctt taaagtcagt tttctgtcag ctctggaaga atatactaaa
      661 aagctgaata cccagtaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      721 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      781 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggntttttgc tgaaaggagg
      841 aactatatcc ggattggcga atgggacgcg ccctgtancg gcgcatttaa gcgcggcggg
      901 ngtggtggnt acgcgcancg tgaccgctac acttgncagc ggccctancg cccgctcctt
      961 tngctttctt cccnttcctt tctncgccan nntncnnccn gntttccccg tccaagctnn
     1021 naaat
//
