LOCUS       sequence-23709-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ49 (cg11094 utr)
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             536..577
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             587..604
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(627..644)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    646..780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(670..689)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     724..743
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gagttcctat ctgaatttgt caagtaaaag gtcttcattt ttcgcaagaa gatgcacctg
       61 atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat
      121 tctaggagct ctgataattt ttcattaact agagtaacga atactacttt gccccgatat
      181 ttattattgt tcagcatcac atattagctt aatgcttcgg tgaaatcgcg cgaatttaac
      241 ttttataact tagagttgag taacttagag ttttatggag caaaacctct gtaaataaat
      301 cgaatttatc ggtaaactaa agcgcgactt ggactatctt caatcaacaa gccaaatatg
      361 tcgatgtgtg acagccgttc tacgcgtcag ctttcttcaa tcaacattac cccgtgctga
      421 gatgtctggc ctcaatgtta ataatctcaa tctacaatca acattctctt ctcttcaatc
      481 aacaatccgc aaacggatct aatcgcggcc gctcgagtct agagggccct tcgaaggtaa
      541 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
      601 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaagatcc agacatgata
      661 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      721 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      781 ctagagtcgg tgggcctcgg gggcgggtgc ggggtcggcg gggccgcccc gggtggcttc
      841 ggtcggagcc atggggtcgt gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc
      901 gtcaggcacc gggctggcgg gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt
      961 cggcggtgac ggacac
//
