LOCUS       sequence-23710-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ50 (cg11094 utr mut)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             526..567
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             577..594
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(617..634)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    636..770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(660..679)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     714..733
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgaatttgt caagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 ctgataattt ttcattaact agagtaacga atactacttt gccccgatat ttattattgt
      181 tcagcatcac atattagctt aatgcttcgg tgaaatcgcg cgcagtgaac ttttataact
      241 tagagttgag taacttagag ttttatggag caaaacctct gtaaataaat cgcagtgatc
      301 ggtaaactaa agcgcgactt ggactatctt caatcaacaa gccaaatatg tcgatgtgtg
      361 acagccgttc tacgcgtcag ctttcttcaa tcaacattac cccgtgctga gatgtctggc
      421 ctcaatgtta ataatctcaa tctacaatca acattctctt ctcttcaatc aacaatccgc
      481 aaacggatct aatcgcggcc gctcgagtct agagggccct tcgaaggtaa gcctatccct
      541 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
      601 taaacccgct gatcagcctc gactgtgcct tctaagatcc agacatgata agatacattg
      661 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      721 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt ctagagtcgg
      781 tgggcctcgg gggcgggtgc ggggtcggcg gggccgcccc gggtggcttc ggtcggagcc
      841 atggggtcgt gcgctccttc gcg
//
