LOCUS       sequence-23711-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ51 (cg1849 utr)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             604..645
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             655..672
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(695..712)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    714..848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(738..757)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     792..811
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcagagttcc taatctgaat ttgtcaagta aaaggtcttc atttttcgca agaagatgca
       61 cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa
      121 taattctagg agctccctgg aaatcagact ccggcgaagt tttatgctcg gactcataaa
      181 atcgtgcgac gagtttgaat cacaggccct cgattttcac caggattttt tacaaatccc
      241 agcagaaaac acgaaaactc aaaaactcag cccaaaaaga aaataccaag aaagcaaact
      301 ttagttcaat ttcatttcaa cacaaaacaa caacaacaca atttgtacat agctaactag
      361 ttgtaacact cataactttt tttttttgag aacctatttt tttcgatgga taatatgcga
      421 atttagctat ttttaatcat tatgtttaac tagtcgtcta agcgagaaat caattttttt
      481 gtctagccat aagttttagc gcgaaaagag atctagcaca aaaatcgaat ttgaaacaaa
      541 accaaataaa aaacaaaaat cacacacaaa aagcggccgc tcgagtctag agggcccttc
      601 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat
      661 caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc taagatccag
      721 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      781 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      841 aacaagttct agagtcggtg ggcccgggcg tgcggcgcgg cgccgtgtcg cgactggctg
      901 cctcg
//
