LOCUS       sequence-23712-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ52 (cg1849 utr mut)
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             609..650
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             660..677
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(700..717)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(743..762)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     797..816
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtgggcagaa gttcctaatc tgaatttgtc aagtaaaagg tcttcatttt tcgcaagaag
       61 atgcacctga tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg
      121 aacaataatt ctaggagctc cctggaaatc agactccggc gaagttttat gctcggactc
      181 ataaaatcgt gcgacgagtt tgaatcacag gccctcgatt ttcaccagga ttttttacaa
      241 atcccagcag aaaacacgaa aactcaaaaa ctcagcccaa aaagaaaata ccaagaaagc
      301 aaactttagt tcaatttcat ttcaacacaa aacaacaaca acacaatttg tacatagcta
      361 actagttgta acactcataa cttttttttt ttgagaacct atttttttcg atggataata
      421 tgcgcagtga gctattttta atcattatgt ttaactagtc gtctaagcga gaaatcaatt
      481 tttttgtcta gccataagtt ttagcgcgaa aagagatcta acacaaaaat cgcagtggaa
      541 acaaaaccaa ataaaaaaca aaaatcacac acaaaaagcg gccgctcgag tctagagggc
      601 ccttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc
      661 atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctaaga
      721 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      781 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      841 caataaacaa gtctagagcg ggccggggtg cggcgcgcgc cgtgtcgcga c
//
