LOCUS       sequence-237128-        7393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-Streptomyces shuttle vector for gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7393)
  TITLE     E.coli-Streptomyces shuttle vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGM1192
FEATURES             Location/Qualifiers
     source          1..7393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            466..575
                     /note="incP origin of transfer"
     CDS             891..1691
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDSFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELLAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      1993..2581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2482..2501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2735..2752
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2869..2890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2905..2910,2911..2928,2929..2935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2943..2959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2948..2970
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2967..2983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2967..2983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3075..3098
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     3709..3732
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      complement(3992..4040)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             5755..7197
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgacccgt gccgggccgc agcggatcgc ggcagcagga gctccccgcc
      181 cgccacggct ggctggatgg tcgcctcacg cgcccgcagg ggtgacggcg tgggtgcggc
      241 ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga ccgcgaccac ccctcccttc
      301 ccgcctgccg tctttcaccg cccggccggt ccgcagacgc cgtacggcca ggccacggag
      361 gattacgggc agatggcgcc cacccggcac ccgggctggg gcaccctgga cagcacgaag
      421 gtccgcccca ctcaccgggg cgggccttcg cgttgagcta gagttcccgc cagcctcgca
      481 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
      541 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
      601 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
      661 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaat gcagctcacg
      721 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
      781 atgccggcct ttgaatgggt tcatgtgcag ctccataagc aaaaggggat gataagttta
      841 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
      901 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
      961 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     1021 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctgcg ctgggtccgg
     1081 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     1141 cgtcgcccgt tacaccggac cttggagttg tctctgactc attctggcgc ctgccaaatg
     1201 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     1261 tctctgatcc attgcccctg ccacctcact cgcctgcaag cccggtcgcc cgtgtccatg
     1321 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     1381 ttgccgagtt gttggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     1441 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     1501 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     1561 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     1621 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     1681 agtcgattgg ctgagctcat aagttcctat tccgaagttc ctattcgaaa tgaccgacca
     1741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1861 gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat
     1921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2641 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2701 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2761 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2881 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2941 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattgtac
     3001 gtagacctac gccttgacct tgatgaggcg cgtgagctac aatcaatact cgattagaag
     3061 ggaggtgaca tatgtggagc cacccgcagt tcgagaaggg atccgtctcc aagggcgagg
     3121 aggacaacat ggccatcatc aaggagttca tgcgcttcaa ggtccacatg gagggctccg
     3181 tcaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccgtac gagggcaccc
     3241 agaccgccaa gctgaaggtc accaagggcg gcccgctgcc gttcgcctgg gacatcctgt
     3301 ccccgcagtt catgtacggc tccaaggcct acgtcaagca cccggccgac atcccggact
     3361 acctgaagct gtccttcccg gagggcttca agtgggagcg cgtcatgaac ttcgaggacg
     3421 gcggcgtcgt caccgtcacc caggactcct ccctgcagga cggcgagttc atctacaagg
     3481 tcaagctgcg cggcaccaac ttcccgtccg acggcccggt catgcagaag aagaccatgg
     3541 gctgggaggc ctcctccgag cgcatgtacc cggaggacgg cgccctgaag ggcgagatca
     3601 agcagcgcct gaagctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca
     3661 aggccaagaa gccggtccag ctgccgggcg cctacaacgt caacatcaag ctggacatca
     3721 cctcccacaa cgaggactac accatcgtcg agcagtacga gcgcgccgag ggccgccact
     3781 ccaccggcgg catggacgag ctgtacaagt gaagcttaga tccccgggta ccgagctcga
     3841 attccccaga tctaaagttt tgtcgtcttt ccagacgtta gtaaatgaat tttctgtatg
     3901 aggttttgct aaacaacttt caacagtttc agcggagtga gaatagaaag gaacaactaa
     3961 aggaattgcg aataataatt ttttcacgtt gaaaatctcc aaaaaaaaag gctccaaaag
     4021 gagcctttaa ttgtatcggt ttatcagctt gctttcgagg tgaatttctt aaacagcttg
     4081 ataccgatag ttgcgccgac aatgacaaca accatcgccc acgcataacc gatatattcg
     4141 gtcgctgagg cttgcaggga gtcaaaggcc gcttttgcgg gatctcgtcg aaggcactag
     4201 aggcctatat gcggggatcg accgcgcggg tcccggacgg ggaagagcgg ggagctttgc
     4261 cagagagcga cgacttcccc ttgcgttggt gattgccggt cagggcagcc atccgccatc
     4321 gtcgcgtagg gtgtcacacc ccaggaatcg cgtcactgaa cacagcagcc ggtaggacga
     4381 ccatgactga gttggacacc atcgcaaatc cgtccgatcc cgcggtgcag cggatcatcg
     4441 atgtcaccaa gccgtcgcga tccaacataa agacaacgtt gatcgaggac gtcgagcccc
     4501 tcatgcacag catcgcggcc ggggtggagt tcatcgaggt ctacggcagc gacagcagtc
     4561 cttttccatc tgagttgctg gatctgtgcg ggcggcagaa cataccggtc cgcctcatcg
     4621 actcctcgat cgtcaaccag ttgttcaagg gggagcggaa ggccaagaca ttcggcatcg
     4681 cccgcgtccc tcgcccggcc aggttcggcg atatcgcgag ccggcgtggg gacgtcgtcg
     4741 ttctcgacgg ggtgaagatc gtcgggaaca tcggcgcgat agtacgcacg tcgctcgcgc
     4801 tcggagcgtc ggggatcatc ctggtcgaca gtgacatcac cagcatcgcg gaccggcgtc
     4861 tccaaagggc cagccgaggt tacgtcttct cccttcccgt cgttctctcc ggtcgcgagg
     4921 aggccatcgc cttcattcgg gacagcggta tgcagctgat gacgctcaag gcggatggcg
     4981 acatttccgt gaaggaactc ggggacaatc cggatcggct ggccttgctg ttcggcagcg
     5041 aaaagggtgg gccttccgac ctgttcgagg aggcgtcttc cgcctcggtt tccatcccca
     5101 tgatgagcca gaccgagtct ctcaacgttt ccgtttccct cggaatcgcg ctgcacgaga
     5161 ggatcgacag gaatctcgcg gccaaccgat aagcgcctct gttcctcgga cgctcggttg
     5221 ctcgacctcg attcgtcagt gatgatcaac aggacccaga aatggcacga gcccgggatt
     5281 ggcctcccgg gctcgccgcc cccgaaccgg cgcagcatgc cgagtgagga cgtttgctct
     5341 gtgacgagcg tacgagagaa cagcgcgtcg acaccaccgc gccccgccgg accgaagtgc
     5401 cactgcggca ccccgatcgt ccacatcccc gggaagcgcc ccaaaatcta ctgctcggac
     5461 gcctgccgaa agcgcgcaaa gcgtctcatt gcggaccagt cacgcagcgt agcaagcacg
     5521 actacagcgg gtaatccgga cgcgggcttt agtagtaagg gaaaatctcc gtccactgca
     5581 ggtcagagcg ctgtaactgg aacgggttct accccaaacg agggtgccgc gacagccggg
     5641 cgggaggacc cgcgcgacga gcggttcgcc cggcgggacc ggcaccagac ggtgtcgctg
     5701 aacgaggcgt tcaagggctg cgggacccgg ctcactgccg ggacggccga gctgatgtgg
     5761 aagccgggcg aggcgacgtg gggcaacacg tgccgctgca acaacgtcca cacctgcccg
     5821 tggtgcatga gccgcatcct ggccgtacgg ggctcgaacg tgcagctcgc cgccgacggt
     5881 ctcgcggacg ccgggtacgg gctgcacctt ggcacgaaca cgttgcgcca cttcgagcgc
     5941 atggcgttcg gcaccgtccg caagggaatg cggcacggcc tggtcgcggt cttgcacgac
     6001 ggctggaagg gcgcgtacgg ctcgtccggc cgccgctggc gcacgatgcg cgacgacttc
     6061 gggatcatcg gctacgagcg ggccttcgag gacaccttcg gctggggctc cggctggcac
     6121 ctgcactggc acacgctctg ggtcacccgc gaggtgctcg gcccggacgc ccaggccgcg
     6181 ttccgcgacg cactcgccgg agcctgggcc gccggggtcg aatccgcggg cggctacacc
     6241 gtcagcgaga cgtgcgaccg gcccggttgc tcctgtgagg gcaagggcca cggcaccgac
     6301 gtgcgcccac tcaacggcgc ggacgccgct gacggcgatg cgggcaagca ggcccgctac
     6361 ctctacaagg acggcgacaa gaccaagggc ggcgtcgcca agatcggcct ggagctggcc
     6421 ggacagaact tcaaggccgg tcgcggcgac gaccgcatgg gaccgctcga cctgggcgac
     6481 gcggcggccg ccgagctgca gcggcttcgt cggcccggcc cgttcgtcga gaagtaccgc
     6541 gagcgcgagt tcggcgtctt ccaggtccgc aagcactacc ggtcgcagaa cctgaaccgg
     6601 ctgatcaagg aactcggcat ccagcaggac gtccgcaccg aggaggagat caccgacgac
     6661 accgagggcc tggtcgccat cgcggtcatc ccggcctaca tctggtaccg atacatcgcc
     6721 cgcgtcgccg gacgtcgcct cgacctcatc aaggtcgccg agacgtacgg cctaccgggc
     6781 gtccggcggc tcgtcgagtc ctggggcctg gtgtggggca aggacgtcct ggacccgccc
     6841 gccccggaag cgccggccgc gccgggcgac cttgacgctg accagatgag gttcgaggtc
     6901 atgtccgagg aggaagctgc tttccgcgag gcgcgccgga aggccaacga ggcccgcacg
     6961 gaggagctgg ccgcttcgct cgaccgagtg cgtcagccga agaaggaggc gatccgcccg
     7021 acgatcagcc ttcggaagcg cctgaagccg aagccggtca ccgtcgacgt caagactccc
     7081 ccgcccggcg ccgcgtcgcc cgtgtgccgc cgctgcaagg gcaagctcgc cccggtactc
     7141 cagccctggg gccagcaccc cggagactgc ctccgcgtcg acacagcggt cgcgtagggc
     7201 cgctgaggga ccgccatgag accgccaccc cggacatccg ggtggcggtc tcatggcggt
     7261 cccggcgcga aagccgaggg tctgcctgcc gtgaggtggc cggcggacct gggttactgc
     7321 tggaatcccg cgcccagtcc gagcgtccgc cggcgctttt cacctggctc cgggtgctcg
     7381 tcgatgagca cca
//
