LOCUS       sequence-237137-       10300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CURT_flouB controlled by the CaMV 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 10300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pN_35S/CURT_flouB
FEATURES             Location/Qualifiers
     source          1..10300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        759..1103
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1047..1067
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1527..1529,1530..2243)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1569..1590)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1830..1849)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2177..2198
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2246..2270)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2590..2614
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3945..4574
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5003..6076
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6142..6336
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6572..6594)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6680..6820
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6735..6754
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6835..6852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7006..7594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7086..7105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7840..8631)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    9152..9176
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9269..9521)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9544..10116)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(join(10158..10300,1..37))
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 aacccgcggc tcagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg
       61 cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc
      121 gataattcga gctctcccat atggtcgact agagccaagc tgatctcctt tgccccggag
      181 atcaccatgg acgactttct ctatctctac gatctaggaa gaaagttcga cggagaaggt
      241 gacgatacca tgttcaccac cgataatgag aagattagcc tcttcaattt cagaaagaat
      301 gctgacccac agatggttag agaggcctac gcggcaggtc tcatcaagac gatctacccg
      361 agtaataatc tccaggagat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
      421 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
      481 attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat agagattgga
      541 gtctctaaga aagtagttcc tactgaatca aaggccatgg agtcaaaaat tcagatcgag
      601 gatctaacag aactcgccgt gaagactggc gaacagttca tacagagtct tttacgactc
      661 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctcgt ctactccaag
      721 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
      781 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca
      841 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt
      901 caagatgccc ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      961 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     1021 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     1081 agttcatttc atttggagag gactccggta tttttacaac aattaccaca acaaaacaaa
     1141 caacaaacaa cattacaatt tactattcta gtcgacctgc aggcggccgc actagtgata
     1201 tcatggcttc ttcagaagaa acatcgtcca ttgataccaa cgaactcatc acagatttga
     1261 aggaaaagtg ggatggtctt gagaacaaat ctactgtact tatatatgga ggaggagcca
     1321 ttgttgctgt ttggttatct tccattgttg ttggtgccat caactctgtt cctctgcttc
     1381 cgaaagttat ggaacttgtc ggactagggt acactggatg gtttgtctac agataccttc
     1441 tcttcaagtc aagcagaaag gaattggctg aggatattga atccttgaag aagaagatcg
     1501 caggaagcga aggaggtggt ggatctgtga gcaagggcga ggagctgttc accggggtgg
     1561 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1621 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1681 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg atgtgcttcg
     1741 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1801 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1861 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1921 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1981 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2041 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2101 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
     2161 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2221 gcatggacga gctgtacaag taatcgttca gctttcttgt acaaagtggt gatatcccac
     2281 ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt
     2341 tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt
     2401 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt
     2461 ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagg catgcgacgt
     2521 cgggcccaag cttagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact
     2581 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2641 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2701 ccaaccacag ggttcccctc gggatcaaag tactttaaag tactttaaag tactttaaag
     2761 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     2821 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     2881 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     2941 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     3001 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     3061 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     3121 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     3181 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     3241 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     3301 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     3361 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     3421 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     3481 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     3541 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     3601 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     3661 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     3721 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     3781 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     3841 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     3901 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     3961 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     4021 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     4081 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     4141 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     4201 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     4261 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     4321 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     4381 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     4441 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     4501 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     4561 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     4621 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     4681 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     4741 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     4801 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     4861 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     4921 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
     4981 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     5041 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     5101 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     5161 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     5221 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     5281 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     5341 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     5401 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     5461 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     5521 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     5581 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     5641 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     5701 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     5761 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     5821 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     5881 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     5941 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     6001 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     6061 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     6121 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     6181 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     6241 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     6301 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     6361 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     6421 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     6481 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     6541 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     6601 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     6661 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     6721 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     6781 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     6841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7441 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc atgatatatc
     7681 tcccaatttg tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg
     7741 atagtttggc tgtgagcaat tatgtgctta gtgcatctaa cgcttgagtt aagccgcgcc
     7801 gcgaagcggc gtcggcttga acgaatttct agctagacat tatttgccga ctaccttggt
     7861 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     7921 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     7981 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     8041 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     8101 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     8161 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     8221 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     8281 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     8341 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     8401 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     8461 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     8521 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     8581 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccccca tgatgtttaa
     8641 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     8701 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     8761 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     8821 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct tacgaaccga
     8881 acaggcttat gtccactggg ttcgtgcccg aattgatcac aggcagcaac gctctgtcat
     8941 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
     9001 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
     9061 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     9121 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     9181 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg
     9241 aattgaatta tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat
     9301 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     9361 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     9421 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     9481 ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttgact ctaggggtca
     9541 tcattagggg cagggcatgc tcatgtagag cgcctgctcg ccgtccgagg cggtgccgtc
     9601 gtacagggcg gtgtccaggc cgcagagggt gaaccccatc cgccggtacg cgtggatcgc
     9661 cggtgcgttg acgttggtga cctccagcca gaggtgcccg gcaccccgct cgcgggcgaa
     9721 ctccgtcgcg agccccatca acgcgcgccc gaccccgtgc ccccggtgct ccggggcgac
     9781 ctcgatgtcc tcgacggtca gccggcggtt ccagccggag tacgagacga ccacgaagcc
     9841 cgccaggtcg ccgtcgtccc cgtacgcgac gaacgtccga gagtccgggt cgccgtcctc
     9901 cccgtcgtcc gattcgtcgt ccgattcgtc gtcggggaac accttggtca ggggcgggtc
     9961 caccggcacc tcccgcaggg tgaagccgtc cccggtggcg gtgacgcgga agacggtgtc
    10021 ggtggtgaag gacccatcca gtgcctcgat ggcctcggcg tcccccggga cactggtgcg
    10081 gtaccggtaa gccgtgtcgt caagagtggt acccatggta gatcccctcg atcgagttga
    10141 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
    10201 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
    10261 atggtttctg acgtatgtgc ttagctcatt aaactccaga
//
