LOCUS       sequence-237147-        2837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with
            GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA
            assembly for A. tumefaciens-mediated genetic transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB007
FEATURES             Location/Qualifiers
     source          1..2837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..24
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      813..870
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(894..911)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1065..1653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1145..1164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1836..1930)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1951..2610)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2525..2544
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2772..2790
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2794..2837)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tggaattcgc ggccgcttct agagcgatga gtggtctcgg gagtggcgca tgcggacaga
       61 cggacggacg cagagagaag ggctgagtaa taagcgccac tgcgccagac agctctggcg
      121 gctctgaggt gcagtggatg attattaatc cgggaccggc cgcccctccg ccccgaagtg
      181 gaaaggctgg tgtgcccctc gttgaccaag aatctattgc atcatcggag aatatggagc
      241 ttcatcgaat caccggcagt aagcgaagga gaatgtgaag ccaggggtgt atagccgtcg
      301 gcgaaatagc atgccattaa cctaggtaca gaagtccaat tgcttccgat ctggtaaaag
      361 attcacgaga tagtaccttc tccgaagtag gtagagcgag tacccggcgc gtaagctccc
      421 taattggccc atccggcatc tgtagggcgt ccaaatatcg tgcctctcct gctttgcccg
      481 gtgtatgaaa ccggaaaggc cgctcaggag ctggccagcg gcgcagaccg ggaacacaag
      541 ctggcagtcg acccatccgg tgctctgcac tcgacctgct gaggtccctc agtccctggt
      601 aggcagcttt gccccgtctg tccgcccggt gtgtcggcgg ggttgacaag gtcgttgcgt
      661 cagtccaaca tttgttgcca tattttcctg ctctccccac cagctgctct tttcttttct
      721 ctttcttttc ccatcttcag tatattcatc ttcccatcca agaaccttta aaatgtgaga
      781 ccactcatcg cgactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca
      841 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
      901 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      961 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1021 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
     1081 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1141 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1201 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1261 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1321 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1381 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1441 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1501 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1561 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1621 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1681 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1741 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1801 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg
     1861 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     1921 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact cgtcgcagta
     1981 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     2041 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     2101 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     2161 ccagggattg gctgatacga aaaacatatt ctcaataaac cctttaggga aataggccag
     2221 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     2281 gtggtattca ctccagagtg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     2341 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     2401 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     2461 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     2521 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     2581 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     2641 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     2701 tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat
     2761 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     2821 tcgctaagga tgatttc
//
