LOCUS       sequence-237150-        2483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator paf from P. chrysogenum domesticated into pUPD2 with 
            GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA
            assembly for A. tumefaciens-mediated genetic transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB030
FEATURES             Location/Qualifiers
     source          1..2483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..58)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    493..514
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      517..560
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(564..582)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             744..1403
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(810..829)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1424..1518
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1701..2289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2190..2209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2443..2460
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcttttcggt tttaaagaaa aagggcaggg tggtgacacc ttgccctttt ttgccggact
       61 gcagcggccg ctactagtcg cgatgagtgg tctcaagcgg cagcagtttg atagttatcc
      121 ctctagccta tttctgctta caatgtacat gtgtcgtatc atagtactcc atttcacttc
      181 cgactacgat caaaagatgc tggatatatt cgataatcta caaccatttc gttgatgagc
      241 aacaatatct agctagatat caaacgagtg tctttatgaa ataagaaaca aaattcgccg
      301 aaaattgaag ctggatagat actgggtgta tatgtatcac ccaccgagca gtccttggtt
      361 cgagtccttg tgggtgtagg gtagtaagct tcgagaatca cagggcatct cgcatatcac
      421 caacaagaag ggaaccaaaa accattaaat gccctggtga tcgcagacac cataagccga
      481 gaccactcat cgctctagaa gcggccgcga attccagaaa tcatccttag cgaaagctaa
      541 ggattttttt tatctgaaat tctgcctcgt gatacgccta tttttatagg ttaatgtcat
      601 gataataatg gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt
      661 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      721 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      781 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      841 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      901 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      961 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     1021 ccgttttcca tgagcaaact gaaacgtttt catcactctg gagtgaatac cacgacgatt
     1081 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     1141 atttccctaa agggtttatt gagaatatgt ttttcgtatc agccaatccc tgggtgagtt
     1201 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     1261 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     1321 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1381 acgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat
     1441 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     1501 ttttttattg gtgagaatcc aagcctcgag ctgtcagacc aagtttactc atatatactt
     1561 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1621 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2281 ctgtggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2401 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2461 gaagcctgca taacgcgaag taa
//
