LOCUS       sequence-23716-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ58 (cg6551 utr mut)
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             802..843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             853..870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(893..910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcagaagttc ctatctgaat ttgtcaagta aaaggtcttc atttttcgca agaagatgca
       61 cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa
      121 taattctagg agctccgtac atctcgttta gcttggacac atccttgtcg gagaggccgc
      181 gtctctggcc cagcgaggcg tacggatcct ggaagcaaag gacaggagtc agagagtgta
      241 gacgatgcaa gctcccccaa gtcattacaa acatctcatc gcttcaacta aaactgctaa
      301 ggtgtagtca accattcagc taaagaattc tcatttgata tagttccaac tggacaagga
      361 agcaaaatgc ttcgtctatt cctagcatag ttgcacgtca ttggaccatg tgttaatata
      421 ggaattccta atctgaatat gatcaaatca ctatcactat cgcagtgaaa tattttatga
      481 gccacggttt ttggcattcc taatggaatc taagttaatt gataggtgtt tttgaatgca
      541 catattcaag tgtggtttgt gcaaaataga ttgatagcat tatagtttgt atatcgaaat
      601 ataacattta ggtgtagtaa ttgttaagat aagcgcagtg actgtatgtc catacaatag
      661 tccattaatt cgcagtatta ctattgaatg ttaaactgaa ctcctttaca gtgtaagcga
      721 tttaaaataa tactcaatta catgaataac tggtaaccat ttgggttggc gcggccgctc
      781 gagtctagag ggcccttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
      841 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
      901 gtgccttcta agatccagac atgataagat acattgatga gttgncaaca cactagangc
      961 gtgaaatgtt tga
//
