LOCUS       sequence-237160-       14459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  conditional expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Porter EG, Dykhuizen EC
  TITLE     Individual Bromodomains of Polybromo-1 Contribute to Chromatin 
            Association and Tumor Suppression in Clear Cell Renal Carcinoma.
  JOURNAL   J Biol Chem. 2017 Jan 4. pii: jbc.M116.746875. doi: 
            10.1074/jbc.M116.746875.
  PUBMED    28053089
REFERENCE   2  (bases 1 to 14459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Jan 4. pii: jbc.M116.746875. doi: 
            10.1074/jbc.M116.746875."
COMMENT     Sequence Label: Fuw PBRM1 mBD5
FEATURES             Location/Qualifiers
     source          1..14459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    168..285
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(342..360,361..383,384..402,403..425,426..444,445..467,
                     468..486,487..509,510..528,529..551,552..570,571..593,
                     594..612)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        645..683
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..704
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      806..815
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5736..6235
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5771..5794)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6168..6187
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6256..6855
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6256..6275)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6752..6772
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6879..7467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6932..6952)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7350..7361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7470..7486)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7471..7487)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7992..8172
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8198..8215)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8204..8428
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8474..8902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8561..8580)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8771..8792
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8911..8931)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8916..9245
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9096..9231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9158..9177
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9293..9340
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9359..9733
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9863..9984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9900..9919)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9954..9973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10033..10049)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10033..10049)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10046..10068)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10057..10073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10081..10087,10088..10105,10106..10111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10126..10147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10264..10281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10435..11023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10515..10534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11194..11985,11986..12054))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11817..11836
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12055..12159)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12234..12253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12425..12804
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12806..13004
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             13022..13202
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13249..13374
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13867..14100
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14285..14329
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
       61 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
      121 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
      181 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      241 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      301 gacagcagag atccagtttg gttagatctc gagtttacca ctccctatca gtgatagaga
      361 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      421 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      481 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      541 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      601 cagtgataga gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt
      661 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
      721 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
      781 tgctagccga tggattcgtg aattcgccac catgggttcc aagagaagaa gagctacctc
      841 cccttccagc agtgtcagcg gggactttga tgatgggcac cattctgtgt caacaccagg
      901 cccaagcagg aaaaggagga gactttccaa tcttccaact gtagatccta ttgccgtgtg
      961 ccatgaactc tataatacca tccgagacta taaggatgaa cagggcagac ttctctgtga
     1021 gctcttcatt agggcaccaa agcgaagaaa tcaaccagac tattatgaag tggtttctca
     1081 gcccattgac ttgatgaaaa tccaacagaa actaaaaatg gaagagtatg atgatgttaa
     1141 tttgctgact gctgacttcc agcttctttt taacaatgca aagtcctatt ataagccaga
     1201 ttctcctgaa tataaagccg cttgcaaact ctgggatttg taccttcgaa caagaaatga
     1261 gtttgttcag aaaggagaag cagatgacga agatgatgat gaagatgggc aagacaatca
     1321 gggcacagtg actgaaggat cttctccagc ttacttgaag gagatcctgg agcagcttct
     1381 tgaagccata gttgtagcta caaatccatc aggacgtctc attagcgaac tttttcagaa
     1441 actgccttct aaagtgcaat atccagatta ttatgcaata attaaggagc ctatagatct
     1501 caagaccatt gcccagagga tacagaatgg aagctacaaa agtattcatg caatggccaa
     1561 agatatagat ctcctcgcaa aaaatgccaa aacttataat gagcctggct ctcaagtatt
     1621 caaggatgca aattcaatta aaaaaatatt ttatatgaaa aaggctgaaa ttgaacatca
     1681 tgaaatggct aagtcaagtc ttcgaatgag gactccatcc aacttggctg cagccagact
     1741 gacaggtcct tcacacagta aaggcagcct tggtgaagag agaaatccca ctagcaagta
     1801 ttaccgtaat aaaagagcag tacaaggagg tcgtttatca gcaattacaa tggcacttca
     1861 atatggctca gaaagtgaag aagatgctgc tttagctgct gcacgctatg aagagggaga
     1921 gtcagaagca gaaagcatca cttcctttat ggatgtttca aatccttttt atcagcttta
     1981 tgacacagtt aggagttgtc ggaataacca agggcagcta atagctgaac ctttttacca
     2041 tttgccttca aagaaaaaat accctgatta ttaccagcaa attaaaatgc ccatatcact
     2101 acaacagatc cgaacaaaac tgaagaatca agaatatgaa actttagatc atttggagtg
     2161 tgatctgaat ttaatgtttg aaaatgccaa acgctataat gtgcccaatt cagccatcta
     2221 caagcgagtt ctaaaattgc agcaagttat gcaggcaaag aagaaagagc ttgccaggag
     2281 agacgatatc gaggacggag acagcatgat ctcttcagcc acctctgata ctggtagtgc
     2341 caaaagaaaa agtaaaaaga acataagaaa gcagcgaatg aaaatcttat tcaatgttgt
     2401 tcttgaagct cgagagccag gttcaggcag aagactttgt gacctattta tggttaaacc
     2461 atccaaaaag gactatcctg attattataa aatcatcttg gagccaatgg acttgaaaat
     2521 aattgagcat aacatccgca atgacaaata tgctggtgaa gagggaatga tagaagacat
     2581 gaagctgatg ttccggaatg ccaggcacta taatgaggag ggctcccagg tttataatga
     2641 tgcacatatc ctggagaagt tactcaagga gaaaaggaaa gagctgggcc cactgcctga
     2701 tgatgatgac atggcttctc ccaaactcaa gctgagtagg aagagtggca tttctcctaa
     2761 aaaatcaaaa tacatgactc caatgcagca gaaactaaat gaggtctatg aagctgtaaa
     2821 gaactatact gataagaggg gtcgccgcct cagtgccata tttctgaggc ttccctctag
     2881 atctgagttg cctgactact atctgactat taaaaagccc atggacatgg aaaaaattcg
     2941 aagtcacatg atggccaaca agtaccaaga tattgactct atggttgagg actttgtcat
     3001 gatgtttaat aatgcctgta cgtacgccga gccggagtct ttgatctaca aagatgctct
     3061 tgttctacac aaagtcctgc ttgaaacacg cagagacctg gagggagatg aggactctca
     3121 tgtcccaaat gtgactttgc tgattcaaga gcttatccac aatctttttg tgtcagtcat
     3181 gagtcatcag gatgatgagg gaagatgcta cagcgattct ttagcagaaa ttcctgctgt
     3241 ggatcccaac tttcctaaca aaccacccct tacatttgac ataattagga agaatgttga
     3301 aaataatcgc taccgtcggc ttgatttatt tcaagagcat atgtttgaag tattggaacg
     3361 agcaagaagg atgaatcgga cagattcaga aatatatgaa gatgcagtag aacttcagca
     3421 gttttttatt aaaattcgtg atgaactctg caaaaatgga gagattcttc tttcaccggc
     3481 actcagctat accacaaaac atttgcataa tgatgtggag aaagagagaa aggaaaaatt
     3541 gccaaaagaa atagaggaag ataaactaaa acgagaagaa gaaaaaagag aagctgaaaa
     3601 gagtgaagat tcctctggtg ctgcaggcct ctcaggctta catcgcacat acagccagga
     3661 ctgtagcttt aaaaacagca tgtaccatgt tggagattac gtctatgtgg aacctgcaga
     3721 ggccaaccta caaccacata tcgtctgtat tgaaagactg tgggaggatt cagctggtga
     3781 aaaatggttg tatggctgtt ggttttaccg accaaatgaa acattccacc tggctacacg
     3841 aaaatttcta gaaaaagaag tttttaagag tgactattac aacaaagttc cagttagtaa
     3901 aattctaggc aagtgtgtgg tcatgtttgt caaggaatac tttaagttat gcccagaaaa
     3961 cttccgagat gaggatgttt ttgtctgtga atcacggtat tctgccaaaa ccaaatcttt
     4021 taagaaaatt aaactgtgga ccatgcccat cagctcagtc aggtttgtcc ctcgggatgt
     4081 gcctctgcct gtggttcgcg tggcctctgt atttgcaaat gcagataaag gtgatgatga
     4141 gaagaataca gacaactcag aggacagtcg agctgaagac aattttaact tggaaaagga
     4201 aaaagaagat gtccctgtgg aaatgtccaa tggtgaacca ggttgccact actttgagca
     4261 gctccattac aatgacatgt ggctgaaggt tggcgactgt gtcttcatca agtcccatgg
     4321 cctggtgcgt cctcgtgtgg gcagaattga aaaagtatgg gttcgagatg gagctgcata
     4381 tttttatggc cccatcttca ttcaccctga agaaacagag catgagccca caaaaatgtt
     4441 ctacaaaaaa gaagtatttc tgagtaatct ggaagaaacc tgccccatga catgtattct
     4501 cggaaagtgt gctgtgttgt cattcaagga cttcctctcc tgcaggccaa ctgaaatacc
     4561 agaaaatgac attctgcttt gtgagagccg ctacaatgag agcgacaagc agatgaagaa
     4621 attcaaagga ttgaagaggt tttcactctc tgctaaagtg gtagatgatg aaatttacta
     4681 cttcagaaaa ccaattgttc ctcagaagga gccatcacct ttgctggaaa agaagatcca
     4741 gttgctagaa gctaaatttg ccgagttaga aggtggagat gatgatattg aagagatggg
     4801 agaagaagat agtgaggtca ttgaacctcc ttctctacct cagcttcaga cccccctggc
     4861 cagtgagctg gacctcatgc cctacacacc cccacagtct accccaaagt ctgccaaagg
     4921 cagtgcaaag aaggaaggct ccaaacggaa aatcaacatg agtggctaca tcctgttcag
     4981 cagtgagatg agggctgtga ttaaggccca acacccagac tactctttcg gggagctcag
     5041 ccgcctggtg gggacagaat ggagaaatct tgagacagcc aagaaagcag aatatgaagg
     5101 catgatgggt ggctatccgc caggccttcc acctttgcag ggcccagttg atggccttgt
     5161 tagcatgggc agcatgcagc cacttcaccc tggggggcct ccaccccacc atcttccgcc
     5221 aggtgtgcct ggcctcccgg gcatcccacc accgggtgtg atgaaccaag gagtggcccc
     5281 tatggtaggg actccagcac cgggtggaag tccatatgga caacaggtgg gagttttggg
     5341 gcctccaggg cagcaggcac cacctccata tcccggccca catccagctg gaccccctgt
     5401 catacagcag ccaacaacac ccatgtttgt agctccccca ccaaagaccc agcggcttct
     5461 tcactcagag gcctacctga aatacattga aggactcagt gcggagtcca acagcattag
     5521 caagtgggat cagacactgg cagctcgaag acgcgacgtc catttgtcga aagaacagga
     5581 gagccgccta ccctctcact ggctgaaaag caaaggggcc cacaccacca tggcagatgc
     5641 cctctggcgc cttcgagatt tgatgctccg ggacaccctc aacattcgcc aagcatacaa
     5701 cctagaaaat gtttaagaat tccataccgg ttaccgggta ggggaggcgc ttttcccaag
     5761 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     5821 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     5881 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     5941 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     6001 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     6061 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     6121 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     6181 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     6241 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6301 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6361 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6421 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6481 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6541 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6601 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6661 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6721 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6781 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6841 aagcccggtg cctgaaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     6901 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6961 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7021 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7081 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7141 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7201 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7261 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7321 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7381 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7441 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     7501 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7561 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7621 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7681 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7741 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7801 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7861 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7921 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7981 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8041 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8101 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8161 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     8221 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8281 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8341 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8401 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     8461 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8521 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8581 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8641 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8701 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8761 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8821 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8881 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8941 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9001 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9061 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9181 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9241 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     9301 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     9361 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     9421 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     9481 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     9541 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     9601 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     9661 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     9721 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     9781 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9841 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9901 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9961 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    10021 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10081 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10141 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10201 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10861 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    11101 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    11161 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    11221 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    11281 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    11341 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    11401 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    11461 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    11521 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11581 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11641 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11701 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11761 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11821 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11881 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11941 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    12001 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    12061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    12121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    12181 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
    12241 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
    12301 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
    12361 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
    12421 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
    12481 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    12541 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    12601 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    12661 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    12721 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    12781 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
    12841 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
    12901 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
    12961 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
    13021 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    13081 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    13141 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    13201 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    13261 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    13321 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    13381 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    13441 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    13501 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    13561 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    13621 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    13681 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    13741 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    13801 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    13861 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    13921 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    13981 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    14041 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    14101 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    14161 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    14221 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    14281 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    14341 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    14401 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatatt
//
