LOCUS       sequence-237162-        5199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 
            origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Liu Y, Landick R, Raman S
  TITLE     A Regulatory NADH/NAD+ Redox Biosensor for Bacteria.
  JOURNAL   ACS Synth Biol. 2019 Feb 15;8(2):264-273. doi: 
            10.1021/acssynbio.8b00485. Epub 2019 Jan 23.
  PUBMED    30633862
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00485"; journalName: "ACS Synth Biol"; date: 
            "2019-02-15- 15"; volume: "8"; issue: "2"; pages: "264-273"
COMMENT     Sequence Label: pB-Rex
FEATURES             Location/Qualifiers
     source          1..5199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(986..1005)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(987..1005)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1015..1032)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1015..1031)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1024..1046)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(1740..2402)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2403..3174
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(4456..4989)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5178..5183,5184..5199,1,2..7))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtccatgt atgcatgagc
       61 tctactacgt cagtgtcgtg ccatagatct cataagggag agcgtcgaga tcccgggcgc
      121 gccttgacaa ttaatcatcc ggctcctagg atgtgtggag ggacgactaa caccagacga
      181 ttctattaca aatccagaat gaataaggat caatcaaaaa ttccgcaggc gacggcgaaa
      241 cggctgccgc tttactatcg ctttttaaag aatctgcatg cgtcaggaaa acagcgtgta
      301 tcatccgctg aactcagtga tgccgtaaag gttgattctg ccacgattcg gagggatttt
      361 tcctattttg gagctcttgg caaaaaagga tacggatata atgtggatta tttgctgtct
      421 tttttccgga aaacgcttga tcaggatgag atgacagacg tcatcttgat tggtgtcggg
      481 aacttgggaa cggcatttct tcactataat ttcacaaaaa acaataacac aaaaatttct
      541 atggcttttg atataaatga gagtaaaata ggaactgagg taggcggcgt gcccgtctat
      601 aaccttgatg accttgaaca acacgtaaaa gatgaatcag ttgccattct tacagtgcca
      661 gcagttgccg ctcaatccat tacagacaga ttggtcgcat taggaatcaa gggaatcctt
      721 aattttacgc cggcccgttt gaatgtgccg gaacacattc gaattcatca tatagattta
      781 gcagttgagc ttcagtcact ggtttatttt ttgaagcatt attcagtttt agaggaaatc
      841 gaatgagctt gatatcgaat tcctgcagcc cgggggatcc ctagttctag aaataagtta
      901 aataaagccc tgagtttaac cgctcggggc tttttgcgtt ttaagcacac tagagcggcc
      961 gccaccgcgg tggagctcca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     1021 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     1081 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     1141 ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa
     1201 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccgt actgcgatga
     1261 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg
     1321 ctacgcctga ataagtgata ataagcggat gaatggcagg aattcgaaag caaattcgac
     1381 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc
     1441 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     1501 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag
     1561 agcctgataa aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
     1621 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcga
     1681 ggcggctaca gccgatagtc tggaacagcg cacttacggg ttgctgcgca acccaagtgc
     1741 taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa
     1801 cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg
     1861 gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg cggctcctcg
     1921 ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg
     1981 ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac
     2041 accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt gaccacgtag
     2101 gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc ggccaccaag
     2161 tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc
     2221 tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg cagcagcatt
     2281 gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt gcgttccgtt
     2341 tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga ctgcgtggcc
     2401 atgcttatct ccatgcggta ggggtgccgc acggttgcgg caccatgcgc aatcagctgc
     2461 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat
     2521 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc
     2581 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg
     2641 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc
     2701 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa
     2761 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg
     2821 gcgggcttcg cccttcgact gcccccactc gcataggctt gggtcgttcc aggcgcgtca
     2881 aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc gccttccact tggtgtccaa
     2941 ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa aattaaaaaa
     3001 attgatgggg caaggccgca ggccgcgcag ttggagccgg tgggtatgtg gtcgaaggct
     3061 gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc tgtccatcag
     3121 cttgtccagc agggttgtcc acgggccgag cgaagcgagc cagccggtgg ccgctcgcgg
     3181 ccatcgtcca catatccacg ggctggcaag ggagcgcagc gaccgcgcag ggcgaagccc
     3241 ggagagcaag cccgtagggc gccgcagccg ccgtaggcgg tcacgacttt gcgaagcaaa
     3301 gtctagtgag tatactcaag cattgagtgg cccgccggag gcaccgcctt gcgctgcccc
     3361 cgtcgagccg gttggacacc aaaagggagg ggcaggcatg gcggcatacg cgatcatgcg
     3421 atgcaagaag ctggcgaaaa tgggcaacgt ggcggccagt ctcaagcacg cctaccgcga
     3481 gcgcgagacg cccaacgctg acgccagcag gacgccagag aacgagcact gggcggccag
     3541 cagcaccgat gaagcgatgg gccgactgcg cgagttgctg ccagagaagc ggcgcaagga
     3601 cgctgtgttg gcggtcgagt acgtcatgac ggccagcccg gaatggtgga agtcggccag
     3661 ccaagaacag caggcggcgt tcttcgagaa ggcgcacaag tggctggcgg acaagtacgg
     3721 ggcggatcgc atcgtgacgg ccagcatcca ccgtgacgaa accagcccgc acatgaccgc
     3781 gttcgtggtg ccgctgacgc aggacggcag gctgtcggcc aaggagttca tcggcaacaa
     3841 agcgcagatg acccgcgacc agaccacgtt tgcggccgct gtggccgatc tagggctgca
     3901 acggggcatc gagggcagca aggcacgtca cacgcgcatt caggcgttct acgaggccct
     3961 ggagcggcca ccagtgggcc acgtcaccat cagcccgcaa gcggtcgagc cacgcgccta
     4021 tgcaccgcag ggattggccg aaaagctggg aatctcaaag cgcgttgaga cgccggaagc
     4081 cgtggccgac cggctgacaa aagcggttcg gcaggggtat gagcctgccc tacaggccgc
     4141 cgcaggagcg cgtgagatgc gcaagaaggc cgatcaagcc caagagacgg cccgagacct
     4201 tcgggagcgc ctgaagcccg ttctggacgc cctggggccg ttgaatcggg atatgcaggc
     4261 caaggccgcc gcgatcatca aggccgtggg cgaaaagctg ctgacggaac agcgggaagt
     4321 ccagcgccag aaacaggccc agcgccagca ggaacgcggg cgcgcacatt tccccgaaaa
     4381 gtgccacctg cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc
     4441 ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc
     4501 ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac
     4561 gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt
     4621 ggcaatgccc tgcctccggt gctctccgga gactgcgaga tcatagatat agatctcact
     4681 acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg
     4741 gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga
     4801 gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag
     4861 agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat
     4921 acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     4981 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     5041 gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg
     5101 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     5161 tacgctactt gcattacagt ttacgaaccg aacaggctt
//
