LOCUS       sequence-237173-        7728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HIV Env gene when transfected into 293-T cells for 
            production of pseudovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Peters PJ, Bhattacharya J, Hibbitts S, Dittmar MT, Simmons G, Bell 
            J, Simmonds P, Clapham PR
  TITLE     Biological analysis of human immunodeficiency virus type 1 R5 
            envelopes amplified from brain and lymph node tissues of AIDS 
            patients with neuropathology reveals two distinct tropism phenotypes
            and identifies envelopes in the brain that confer an enhanced 
            tropism and fusigenicity for macrophages.
  JOURNAL   J Virol. 2004 Jul;78(13):6915-26.
  PUBMED    15194768
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2004-07"; volume: "78(13)"; pages: "6915-26"
COMMENT     Sequence Label: NA20 LN16
FEATURES             Location/Qualifiers
     source          1..7728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             74..707
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     promoter        1976..2171
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2022..2157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2275..2294)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(2442..2460)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2528..2632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2633..2701,2702..3493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2851..2870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3664..4252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4153..4172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4406..4423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4504..4523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    6707..6940
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
       61 gaaaaggggg gactggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
      121 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
      181 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
      241 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
      301 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
      361 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
      421 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
      481 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
      541 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      601 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      661 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
      721 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
      781 ttgacattat aatagattta gcaggaattg aactaggagt ggagcacaca ggcaaagctg
      841 cagaagtact tggaagaagc caccagagat actcacgatt ctgcacatac ctggctaatc
      901 ccagatccta aggattacat taagtttact aacatttata taatgattta tagtttaaag
      961 tataaactta tctaatttac tattctgaca gatattaatt aatcctcaaa tatcataaga
     1021 gatgattact attatcccca tttaacacaa gaggaaactg agagggaaag atgttgaagt
     1081 aattttccca caattacagc atccgttagt tacgactcta tgatcttctg acacaaattc
     1141 catttactcc tcaccctatg actcagtcga atatatcaaa gttatggaca ttatgctaag
     1201 taacaaatta cccttttata tagtaaatac tgagtagatt gagagaagaa attgtttgca
     1261 aacctgaata gcttcaagaa gaagagaagt gaggataaga ataacagttg tcatttaaca
     1321 agttttaaca agtaacttgg ttagaaaggg attcaaatgc ataaagcaag ggataaattt
     1381 ttctggcaac aagactatac aatataacct taaatatgac ttcaaataat tgttggaact
     1441 tgataaaact aattaaatat tattgaagat tatcaatatt ataaatgtaa tttactttta
     1501 aaaagggaac atagaaatgt gtatcattag agtagaaaac aatccttatt atcacaattt
     1561 gtcaaaacaa gtttgttatt aacacaagta gaatactgca ttcaattaag ttgactgcag
     1621 attttgtgtt ttgttaaaat tagaaagaga taacaacaat ttgaattatt gaaagtaaca
     1681 tgtaaatagt tctacatacg ttcttttgac atcttgttca atcattgatc gaagttcttt
     1741 atcttggaag aatttgttcc aaagactctg aaataaggaa aacaatctat tatatagtct
     1801 cacacctttg ttttactttt agtgatttca atttaataat gtaaatggtt aaaatttatt
     1861 cttctctgag atcatttcac attgcagata gaaaacctga gactggggta atttttatta
     1921 aaatctaatt taatctcaga aacacctcta gaattccggc tagagcgatg tcgaggcatc
     1981 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2041 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2101 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2161 gcttttgcaa aaagcttggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     2221 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     2281 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2341 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2401 cgcctgatga atgctcatcc ggaattcttg aagacgaaag ggcctcgtga tacgcctatt
     2461 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2521 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2581 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2641 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2701 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2761 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2821 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     2881 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2941 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3001 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3061 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3121 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     3181 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3241 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3301 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3361 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3421 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3481 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3781 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4321 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4381 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     4441 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     4501 cagtacaatc tgctctgatg ccgcatagtt aagccagtat cctcgaggtc acgtggcccg
     4561 agagctgcat ccggagtact tcaagaactg ttgacatcga gcttgttaca agggactttc
     4621 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     4681 atgctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     4741 gagcctggga gctctctggc tagctaggga acccactgct taagcctcaa taaagcttgg
     4801 gctgcaggtc gaccgttgct ttcattgcca agtttgtttc ataacaaaag gcttaggcat
     4861 ctcctatggc aggaagaagc ggagacagcg acgaagagct cctgaaaaca gtcagactca
     4921 tcaagtttct ctatcaaagc agtaagtagt atatgtaatg cactctttac aaatattagc
     4981 aatagtagca ttagtagtag cagcaataat agcaatagtt gtgtggtcca tagtactcat
     5041 agaatatagg aaaatattaa gacaaagaaa aatagacagg ttaattgata gaataacaga
     5101 aagagcagaa gacagtggca atgagagtga tggggatcag gaagaattat cagcacttgt
     5161 ggaaatgggg cacgatgctc cttgggatgt taatgatctg taatgttgca ggaaatttgt
     5221 gggtcacagt ctattatggg gtgcctgtgt ggaaagaagc aacaaccact ctattttgtg
     5281 catcagatgc taaagcatat gatacagagg tgcataatgt ttgggccaca catgcctgtg
     5341 tacccacaga ccccaaccca caagaagtag tattggtgaa tgtgacagaa gattttaaca
     5401 tgtggaaaaa taacatggta gaacagatgc atgaggatat aattagttta tgggatcaaa
     5461 gcctaaaacc atgtgtaaaa ttaaccccac tctgtgttac tttaaaatgc actgattgga
     5521 aaaggaatgc cactgttaat gccactgtta ccaccactgc tacccctagt agtaacggga
     5581 gaacgctgga gggaggagaa gtgaaaaatt gctctttcaa tatcaccaca agcataagag
     5641 ataaggtgaa gaaagaatat gcactttttt atagaattga tgtagtacca atagaaaatg
     5701 ataatactag atatagattg ataaattgta atacctcagt cattacacag gcctgtccaa
     5761 aggtatcctt tgagccaatt cccatacatt attgtgcccc ggctggtttt gcgattctaa
     5821 agtgtaatga taagaagttc agtggaaaag gatcatgtaa aaatgtcagc acagtacaat
     5881 gtacacatgg aattaggcca gtagtaacaa ctcaattgct gttaaatggc agtctagcag
     5941 aagaagaggt agtaattaaa tctgacaatt tcacggacaa tgctaaaagc ataatagtac
     6001 agctaaatga atctgtagaa attaattgta caagacccaa caacaataca agaaaaagta
     6061 tacatatagg accaggcaga gcattttatg caacaggaga aataacagga gatataagac
     6121 aagcacattg taacattagt agagcaaaat ggaataaaac tttagaacag atagttataa
     6181 agttaagaga acaatttaga gaacaattta agaatagaac aatagaaaca atagtcttta
     6241 atcaatcctc aggaggggac ccagaaattg taatgcacag ttttaattgt ggaggggaat
     6301 ttttctactg caattcaaca caactgttta atagtacttg gaatgttaaa ggtacttgga
     6361 atggtactga agagtcaaat aacaatgaag gaaatgacac aatcacactc caatgcagaa
     6421 taaaacaaat tataaacatg tggcaggaag tagggaaagc aatgtatgcc ccgcccatca
     6481 aaggacaaat tagatgttca tcaaatatta cagggctgat attaacaaga gatggtggta
     6541 atgagaatac caccacagag accttcagac ctggaggagg aaatatgagg gacaattgga
     6601 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     6661 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     6721 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcactgacg ctgacggtac
     6781 aggccagaca attattgtct ggtatagtgc aacagcagaa caatttgctg agggctattg
     6841 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagag
     6901 tcctggctgt agaaagatac ctacaggatc aacagctcct ggggatttgg ggttgctctg
     6961 gaaaactcat ctgcaccacc actgtgcctt ggaatactag ttggagtaat agatctcagg
     7021 aggagatttg gggtaatttg acctggatgc agtgggaaaa agaaattgat aattacacaa
     7081 gcttaatata caccttaatt gaacaatcgc aaaaccaaca agaaaagaat gaacaagaat
     7141 tattagaatt agataaatgg gcaaatttgt ggaattggtt tagcataaca aactggctgt
     7201 ggtatataaa aatattcata atgatagtag gaggcttgat aggtttaaga atagtttttg
     7261 ctgtactttc tatagtgaat agagttaggc agggatactc accattgtcg ttccagatcc
     7321 gccccccagc tccgagggga cccgacaggc ccgaaggaat cgaagaagaa ggtggagaga
     7381 gagacagaga cagatccggt ccattagtgg atggattctt agcaattatc tgggtcgacc
     7441 tgcggagcct gtgcctcttc agctaccacc gcttgagaga ctttctcttg attgtagcga
     7501 ggattgtgga acttctggga cgcagggggt gggaactcct caaatattgg tggaatctcc
     7561 tacagtattg gattcaggaa ctaaagaata gagctattag cttgcttaat gccaccgcca
     7621 caacagtagc tgaggggact gatagggtta tagaagtagt acaaagagct tatagggcta
     7681 ttctccatat acctacacga ataagacagg gcttggaaag ggctttgc
//
