LOCUS       sequence-237179-        9512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-cjCD69
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     323..346
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        393..1144
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             1151..2803
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3154..3171)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3160..3384
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3620..3664)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3849..4082
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4575..4700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4747..4927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4928..5154)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5182..5202)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5182..5200)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5221..5237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5221..5237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5234..5256)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5245..5261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5269..5275,5276..5293,5294..5299))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5314..5335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5452..5469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5623..6211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5703..5722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6382..7173,7174..7242))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7005..7024
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7243..7347)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7373..7828)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7510..7531)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7722..7741
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7955..7977
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7969..7986
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7970..7986
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7996..8015
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7996..8014
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8035..8170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8210..8331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8221..8240)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8275..8294
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8403..8636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8774..9373)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8857..8877)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9354..9373
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(9394..9512,1..381))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9442..9461)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa
       61 gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca
      121 tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct
      181 gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac
      241 caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc
      301 cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg
      361 ggaaaagcgc ctcccctacc cggtagaatt gaaatgagtc ttcggacctc gcgggggccg
      421 cttaagcggt ggttagggtt tgtctgacgc ggggggaggg ggaaggaacg aaacactctc
      481 attcggaggc ggctcggggt ttggtcttgg tggccacggg cacgcagaag agcgccgcga
      541 tcctcttaag cacccccccg ccctccgtgg aggcgggggt ttggtcggcg ggtggtaact
      601 ggcgggccgc tgactcgggc gggtcgcgcg ccccagagtg tgaccttttc ggtctgctcg
      661 cagacccccg ggcggcgccg ccgcggcggc gacgggctcg ctgggtccta ggctccatgg
      721 ggaccgtata cgtggacagg ctctggagca tccgcacgac tgcggtgata ttaccggaga
      781 ccttctgcgg gacgagccgg gtcacgcggc tgacgcggag cgtccgttgg gcgacaaaca
      841 ccaggacggg gcacaggtac actatcttgt cacccggagg cgcgagggac tgcaggagct
      901 tcagggagtg gcgcagctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt
      961 ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg
     1021 tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt cccaggtcca
     1081 cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc
     1141 ttaaggatcc atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact
     1201 cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc
     1261 cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt
     1321 cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg
     1381 gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt
     1441 catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag
     1501 catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct
     1561 caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga
     1621 ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa
     1681 cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa
     1741 cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt
     1801 ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat
     1861 cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat
     1921 ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt
     1981 gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa
     2041 gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc
     2101 gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg
     2161 ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga
     2221 caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga
     2281 caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat
     2341 catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg
     2401 gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg
     2461 gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat
     2521 cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc
     2581 cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga
     2641 gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt
     2701 gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga
     2761 gattctcatt aaggccaaga agggcggcaa gatcgccgtg taattctagt tgtttaaaca
     2821 tacagtgttt tccagggaaa agacaaaagc aaagaatatg aggaatattt gtaaaacagg
     2881 gaaaagtgtt ggaaaatgtg caatatgtga tgtggcaaat ttctatcagg aaatattctg
     2941 taatgttcaa acctagaata atactagtca taataggttt gtgactttcc taatcaattc
     3001 tttaatgtgc aatatttcat ttaaagtatt tttatgtgca ataaaatgta tttgtttgta
     3061 ttttgtgttc agtacaatta taagctgttt ttatgcacgg gaaaataaaa gtagaataaa
     3121 tatctagagt cgacgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     3181 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3241 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3301 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3361 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3421 ggtatcccct cccccctttt cttttgggtc ccctcggggt tgggaggtgg gtctgaaacg
     3481 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     3541 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     3601 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     3661 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     3721 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
     3781 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
     3841 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
     3901 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
     3961 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     4021 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
     4081 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
     4141 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
     4201 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
     4261 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     4321 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     4381 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     4441 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     4501 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     4561 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     4621 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     4681 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     4741 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     4801 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     4861 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     4921 gagacccgtt tatgtatcga gctaggcact taaatacaat atctctgcaa tgcggcaatt
     4981 cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag gcacgatcgt
     5041 accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt
     5101 gctaactcat cgttaccatg ttgcaagact acaagagtat tgcataagac tacattaagc
     5161 ttgcagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     5221 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5281 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5341 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5401 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6061 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6661 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7321 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     7381 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     7441 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     7501 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     7561 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     7621 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     7681 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     7741 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     7801 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     7861 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     7921 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     7981 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacgtc cctaggcctc
     8041 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     8101 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     8161 ggggcgggat gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt
     8221 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     8281 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact
     8341 ctctgatatt catttctttg caagttataa atactgaata ataagatgac atgaactact
     8401 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     8461 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     8521 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     8581 cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgagctagcc cttccagtcc
     8641 ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
     8701 aggtaccggg ccctcgagat gcatggggtc gtgcgctcct ttcggtcggg cgctgcgggt
     8761 cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg
     8821 gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg
     8881 gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga
     8941 gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca
     9001 ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg
     9061 cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc
     9121 ccgcttcgac gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc
     9181 acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc
     9241 gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg
     9301 cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct
     9361 tgtactcggt catggtaagc ttgggctgca ggtcgaaagg cccggagatg aggaagagga
     9421 gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc cggaggacct
     9481 tcgggcgccc gccccgcccc tgagcccgcc cc
//
