LOCUS       sequence-237180-        9578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-cjMGA
FEATURES             Location/Qualifiers
     source          1..9578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             702..2354
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2771..2788)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2777..3001
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3237..3281)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3466..3699
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4192..4317
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4364..4544
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4545..4771)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4799..4819)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4799..4817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4838..4854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4838..4854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4851..4873)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4862..4878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4886..4892,4893..4910,4911..4916))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4931..4952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5069..5086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5240..5828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5320..5339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5999..6790,6791..6859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6622..6641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6860..6964)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6990..7445)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7127..7148)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7339..7358
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7572..7594
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7586..7603
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7587..7603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7613..7632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7613..7631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7652..7787
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7827..7948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7838..7857)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7892..7911
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8020..8253
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8391..8990)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8474..8494)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8971..8990
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(9011..9510)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9059..9078)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9452..9475
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        join(9522..9578,1..695)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
ORIGIN
        1 cggggggagg gggaaggaac gaaacactct cattcggagg cggctcgggg tttggtcttg
       61 gtggccacgg gcacgcagaa gagcgccgcg atcctcttaa gcaccccccc gccctccgtg
      121 gaggcggggg tttggtcggc gggtggtaac tggcgggccg ctgactcggg cgggtcgcgc
      181 gccccagagt gtgacctttt cggtctgctc gcagaccccc gggcggcgcc gccgcggcgg
      241 cgacgggctc gctgggtcct aggctccatg gggaccgtat acgtggacag gctctggagc
      301 atccgcacga ctgcggtgat attaccggag accttctgcg ggacgagccg ggtcacgcgg
      361 ctgacgcgga gcgtccgttg ggcgacaaac accaggacgg ggcacaggta cactatcttg
      421 tcacccggag gcgcgaggga ctgcaggagc ttcagggagt ggcgcagctg cttcatcccc
      481 gtggcccgtt gctcgcgttt gctggcggtg tccccggaag aaatatattt gcatgtcttt
      541 agttctatga tgacacaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag
      601 atgcagtcgg ggcggcgcgg tcccaggtcc acttcgcata ttaaggtgac gcgtgtggcc
      661 tcgaacaccg agcgaccctg cagcgacccg cttaaggatc catggaagat gccaaaaaca
      721 ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc
      781 acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata
      841 tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta
      901 tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc
      961 agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg
     1021 acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat
     1081 tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac
     1141 aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct
     1201 tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg
     1261 accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg
     1321 gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct
     1381 tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct
     1441 tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc
     1501 gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc
     1561 tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa
     1621 gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg
     1681 tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca
     1741 gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc
     1801 ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc
     1861 gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg
     1921 ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg
     1981 acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct
     2041 accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg
     2101 ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc
     2161 tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta
     2221 caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga
     2281 ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca
     2341 agatcgccgt gtaattctag ttgtttagcg ctagctaccc attaacctta ccctagggga
     2401 aactgatcta tgaatattac atgtgtgatt atgaaaggtg aagttgagtg agaggagttg
     2461 aggaacctgg aggacagtga gctctcaccc taataccata agaagcatga tctcggtaga
     2521 ccaataattc gcctttttat acatctgtaa ataaatggaa tgtttttaag aatataatgt
     2581 cagatttagc atttttcttt tatatattaa atatatatat ctttcctttt ctgctttcga
     2641 aaagtgacta tttttttaga aacctaagaa cagaagtttg gtgattgaaa ggtagaatat
     2701 ataaacctga aataaatcag ttacaagaat taaagcagtt ctagagtcga cgctagagct
     2761 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2821 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     2881 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     2941 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3001 gcttctgagg cggaaagaac cagctggggc tctagggggt atcccctccc cccttttctt
     3061 ttgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     3121 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     3181 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     3241 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     3301 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     3361 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     3421 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     3481 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     3541 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     3601 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     3661 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     3721 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     3781 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     3841 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     3901 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     3961 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     4021 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     4081 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     4141 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     4201 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     4261 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     4321 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     4381 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     4441 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     4501 ccagagagct cccaggctca gatctggtct aaccagagag acccgtttat gtatcgagct
     4561 aggcacttaa atacaatatc tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc
     4621 agacccgtct gttgccttcc taataaggca cgatcgtacc accttacttc caccaatcgg
     4681 catgcacggt gctttttctc tccttgtaag gcatgttgct aactcatcgt taccatgttg
     4741 caagactaca agagtattgc ataagactac attaagcttg cagctccagc ttttgttccc
     4801 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4861 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4921 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4981 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5041 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5101 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5161 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5281 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5401 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5461 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5521 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5581 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5641 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5701 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5761 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5821 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5881 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5941 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6001 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6061 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6961 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     7021 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7081 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7141 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     7201 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     7261 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     7321 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     7381 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     7441 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     7501 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     7561 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     7621 ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc
     7681 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     7741 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     7801 ggcgggatag ctagagccag acatgataag atacattgat gagtttggac aaaccacaac
     7861 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     7921 aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa
     7981 gttataaata ctgaataata agatgacatg aactactact gctagagatt ttccacactg
     8041 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     8101 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     8161 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat
     8221 cttgtcttcg ttgggagtga gctagccctt ccagtccccc cttttctttt aaaaagtggc
     8281 taagatctac agctgccttg taagtcattg gtcttaaagg taccgggccc tcgagatgca
     8341 tggggtcgtg cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg tcaggcaccg
     8401 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
     8461 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
     8521 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
     8581 ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc
     8641 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
     8701 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
     8761 gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
     8821 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg
     8881 acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg
     8941 gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtaagcttg
     9001 ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct
     9061 tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga
     9121 gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga
     9181 aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg
     9241 tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg
     9301 acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga
     9361 aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag
     9421 gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag
     9481 actgccttgg gaaaagcgcc tcccctaccc ggtagaattg aaatgagtct tcggacctcg
     9541 cgggggccgc ttaagcggtg gttagggttt gtctgacg
//
