LOCUS       sequence-237181-        3522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component 
            MultiSite Gateway Pro assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, 
            Newgard CB
  TITLE     Creation of versatile cloning platforms for transgene expression and
            dCas9-based epigenome editing.
  JOURNAL   Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 
            10.1093/nar/gky1286.
  PUBMED    30590691
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286."
COMMENT     Sequence Label: IG510: pMVP (R4-R3) human PDX1
FEATURES             Location/Qualifiers
     source          1..3522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(603..726)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1585..1708
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1763..1782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1764..1782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1787..1803)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1787..1803)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1905..1924)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1916..2725
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1983..2002)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2872..3460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3361..3380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga
      601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
      661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca
      721 aagttgccac catgaacggc gaggagcagt actacgcggc cacgcagctt tacaaggacc
      781 catgcgcgtt ccagcgaggc ccggcgccgg agttcagcgc cagcccccct gcgtgcctgt
      841 acatgggccg ccagcccccg ccgccgccgc cgcacccgtt ccctggcgcc ctgggcgcgc
      901 tggagcaggg cagccccccg gacatctccc cgtacgaggt gccccccctc gccgacgacc
      961 ccgcggtggc gcaccttcac caccacctcc cggctcagct cgcgctcccc cacccgcccg
     1021 ccgggccctt cccggaggga gccgagccgg gcgtcctgga ggagcccaac cgcgtccagc
     1081 tgcctttccc atggatgaag tctaccaaag ctcacgcgtg gaaaggccag tgggcaggcg
     1141 gcgcctacgc tgcggagccg gaggagaaca agcggacgcg cacggcctac acgcgcgcac
     1201 agctgctaga gctggagaag gagttcctat tcaacaagta catctcacgg ccgcgccggg
     1261 tggagctggc tgtcatgttg aacttgaccg agagacacat caagatctgg ttccaaaacc
     1321 gccgcatgaa gtggaaaaag gaggaggaca agaagcgcgg cggcgggaca gctgtcgggg
     1381 gtggcggggt cgcggagcct gagcaggact gcgccgtgac ctccggcgag gagcttctgg
     1441 cgctgccgcc gccgccgccc cccggaggtg ctgtgccgcc cgctgccccc gttgccgccc
     1501 gagagggccg cctgccgcct ggccttagcg cgtcgccaca gccctccagc gtcgcgcctc
     1561 ggcggccgca ggaaccacga tgagcaactt tgtataataa agttgaacga gaaacgtaaa
     1621 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     1681 gtaaaacaca acatatccag tcactatgaa tcaactactt agatggtatt agtgacctgt
     1741 actgcagggc ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     1801 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1861 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1921 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     1981 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2041 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2101 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2161 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     2221 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2281 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2341 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2401 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2461 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2521 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2581 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     2641 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     2701 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     2761 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     2821 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2881 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2941 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3001 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3061 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3121 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3181 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3241 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3301 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3361 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3421 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3481 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
