LOCUS       sequence-237227-        8436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of neurotensin receptor fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Grisshammer R, Tucker J
  TITLE     Quantitative evaluation of neurotensin receptor purification by 
            immobilized metal affinity chromatography.
  JOURNAL   Protein Expr Purif. 1997 Oct;11(1):53-60. doi: 
            10.1006/prep.1997.0766.
  PUBMED    9325139
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1006/prep.1997";
            journalName: "Protein Expr Purif"; date: "1997-10"; volume: "11"; 
            issue: "1"; pages: "53-60"
COMMENT     Sequence Label: pRG/III-hs-MBP-T43NTR-TrxA-H10
FEATURES             Location/Qualifiers
     source          1..8436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        285..362
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             363..1445
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(382..401)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1458..1479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1494..1499,1500..1517,1518..1524)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1532..1548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1537..1559
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1556..1572
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1556..1572
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(1608..1685,1686..2783)
                     /codon_start=1
                     /gene="malE"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This gene encodes a signal sequence that can direct 
                     MBP to the bacterial periplasm."
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNG
                     LAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAE
                     ITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDK
                     ELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLV
                     DLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPS
                     KPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEVLAKD
                     PRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2640..2649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2757..2780
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3945..4268
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     CDS             4275..4304
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(4326..4343)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4326..4342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4335..4357)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4521..4538)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4691..4777
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4869..4896
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4915..5006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5007..5075,5076..5867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5225..5244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     6935..6954
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7152..7740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7641..7660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7894..7911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8077..8099
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8093..8284)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
       61 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
      121 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
      181 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
      241 atcatgcgca cccgtggcca ggacccaacg ctgcccgaaa ttccgacacc atcgaatggt
      301 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
      361 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctck tatcagaccg
      421 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
      481 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
      541 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
      601 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
      661 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
      721 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
      781 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
      841 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
      901 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
      961 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
     1021 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
     1081 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
     1141 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
     1201 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca
     1261 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     1321 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
     1381 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     1441 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     1501 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     1561 aacagctatg accatgatta cgaatttcta gataacgagg gcaaaccatg aaaataaaaa
     1621 caggtgcacg catcctcgca ttatccgcat taacgacgat gatgttttcc gcctcggctc
     1681 tcgccaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg
     1741 gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc
     1801 atccggataa actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca
     1861 ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa
     1921 tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt
     1981 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
     2041 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
     2101 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
     2161 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta
     2221 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
     2281 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
     2341 aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca
     2401 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg
     2461 ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt
     2521 tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc
     2581 tgggtgccgt agcgctgaag tcttacgagg aagtgttggc gaaagatcca cgtattgccg
     2641 ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt
     2701 tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg
     2761 aagccctgaa agacgcgcag actggatcca cctcggaatc cgacacggca gggcccaaca
     2821 gcgacctgga cgtgaacact gacatttatt ccaaggtgct ggtgactgct atatacctgg
     2881 cactcttcgt ggtgggcact gtgggcaact ccgtgacagc cttcactcta gcgcggaaga
     2941 agtcactgca gagcctgcag agcactgtgc attaccacct gggcagcctg gcactgtctg
     3001 acctgcttat ccttctgctg gccatgcccg tggagctata caacttcatc tgggtacacc
     3061 atccctgggc ctttggggac gctggctgcc gtggctacta tttcctgcgt gatgcctgca
     3121 cctatgccac agccctcaat gtagccagcc tgagtgtgga gcgctacttg gccatctgcc
     3181 atcccttcaa ggccaagacc ctcatgtccc gcagccgcac caagaaattc atcagtgcca
     3241 tatggctagc ttcggcgctg ctggctatac ccatgctttt caccatgggc ctgcagaacc
     3301 gcagtggtga cggcacgcac cctggcggcc tggtgtgcac acccattgtg gacacagcca
     3361 ctgtcaaggt cgtcatccag gttaacacct tcatgtcctt cctgtttccc atgttggtca
     3421 tctccatcct aaacaccgtg attgccaaca aactgacagt catggtgcac caggccgccg
     3481 agcagggccg agtgtgcacc gtgggcacac acaacggttt agagcacagc acgttcaaca
     3541 tgaccatcga gccgggtcgt gtccaggccc tgcgccacgg agtcctcgtc ttacgtgctg
     3601 tggtcattgc ctttgtggtc tgctggctgc cctaccacgt gcgacgcctg atgttctgct
     3661 atatctcgga tgaacagtgg actacgttcc tcttcgattt ctaccactat ttctacatgc
     3721 taaccaacgc tctcttctac gtcagctccg ccatcaatcc catcctctac aacctggtct
     3781 ccgccaactt ccgccaggtc tttctgtcca cgctggcctg cctttgtcct gggtggcgcc
     3841 accgccgaaa gaagaggcca acgttctcca ggaagcccaa cagcatgtcc agcaaccatg
     3901 ccttttccac cagcgccacc cgggagaccc tgtacgcggc cgcaagcgat aaaattattc
     3961 acctgactga cgacagtttt gacacggatg tactcaaagc ggacggggcg atcctcgtcg
     4021 atttctgggc agagtggtgc ggtccgtgca aaatgatcgc cccgattctg gatgaaatcg
     4081 ctgacgaata tcagggcaaa ctgaccgttg caaaactgaa catcgatcaa aaccctggca
     4141 ctgcgccgaa atatggcatc cgtggtatcc cgactctgct gctgttcaaa aacggtgaag
     4201 tggcggcaac caaagtgggt gcactgtcta aaggtcagtt gaaagagttc ctcgacgcta
     4261 acctggcggg tacccaccat caccatcacc atcaccatca ccattaataa tctagaaagc
     4321 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4381 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4441 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcagcttgg ctgttttggc
     4501 ggatgagata agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
     4561 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     4621 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
     4681 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     4741 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     4801 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     4861 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt
     4921 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4981 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5041 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5101 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5161 gtaagatcct tgagagtttt cgccccgaag aacgtttccc aatgatgagc acttttaaag
     5221 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     5281 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5341 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5401 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5461 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5521 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5581 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5641 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5701 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5761 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5821 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5881 tttactcata tatactttag attgatttac cccggttgat aatcagaaaa gccccaaaaa
     5941 caggaagatt gtataagcaa atatttaaat tgtaaacgtt aatattttgt taaaattcgc
     6001 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6061 ttataaatca aaagaatagc ccgagatccg gccgaacaaa ctccgggagg cagcgtgatg
     6121 cggcaacaat cacacggatt tcccgtgaac ggtctgaatg agcggattat tttcagggaa
     6181 agtgagtgtg gtcagcgtgc aggtatatgg gctatgatgt gcccggcgct tgaggctttc
     6241 tgcctcatga cgtgaaggtg gtttgttgcc gtgttgtgtg gcagaaagaa gatagccccg
     6301 tagtaagtta attttcatta accaccacga ggcatcccta tgtctagtcc acatcaggat
     6361 agcctcttac cgcgctttgc gcaaggagaa gaaggccatg aaactaccac gaagttccct
     6421 tgtctggtgt gtgttgatcg tgtgtctcac actgttgata ttcacttatc tgacacgaaa
     6481 atcgctgtgc gagattcgtt acagagacgg acacagggag gtggcggctt tcatggctta
     6541 cgaatccggt aagtagcaac ctggaggcgg gcgcaggccc gccttttcag gactgatgct
     6601 ggtctgacta ctgaagcgcc tttataaagg ggctgctggt tcgccggtag cccctttctc
     6661 cttgctgatg ttgtgggaat tcctgaatcg ccccatcatc cagccagaaa gtgagggagc
     6721 cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg
     6781 ccacggaacg gtctgcgttg tcgggaagat gtgaaccatc acccaaatca agttttttgg
     6841 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6901 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     6961 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     7021 atgcgccgct acagggcgcg taaaaggatc taggtgaaga tcctttttga taatctcatg
     7081 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7141 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7201 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7261 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7321 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7381 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7441 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7501 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7561 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7621 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7681 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7741 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7801 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7861 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7921 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg
     7981 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8041 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8101 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     8161 gcagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     8221 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     8281 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     8341 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
     8401 gtgagggtaa acaactggcg gtatggatgc ggcggg
//
