LOCUS       sequence-237230-       10779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses recombinant MVA pathway in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Jervis AJ, Carbonell P, Vinaixa M, Dunstan MS, Hollywood KA, 
            Robinson CJ, Rattray NJW, Yan C, Swainston N, Currin A, Sung R, 
            Toogood HS, Taylor S, Faulon JL, Breitling R, Takano E, Scrutton NS
  TITLE     Machine learning of designed translational control allows predictive
            pathway optimisation in Escherichia coli.
  JOURNAL   ACS Synth Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398.
  PUBMED    30563328
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398."
COMMENT     Sequence Label: pMVA2RBS035
FEATURES             Location/Qualifiers
     source          1..10779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      614..661
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      695..766
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      782..809
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(837..854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(971..1516)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1051..1070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1630..1724)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1755..2549)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1866..1885)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2476..2495
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        2692..2769
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2770..3852
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2789..2808)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(4084..4089,4090..4106,4107..4113)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4121..4137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(7779..7784,7785..7801,7802..7808)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    7816..7832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gctaaggagt cgcacgagac gccaaattgg gaggtgggcg atgcagaccg aacatgttat
       61 tctgctgaat gcacagggtg ttccgaccgg caccctggaa aaatatgcag cacataccgc
      121 agatacccgt ctgcatctgg catttagcag ctggctgttt aatgcaaaag gtcagctgct
      181 ggttacccgt cgtgcactga gcaaaaaagc atggcctggt gtttggacca atagcgtttg
      241 tggtcatccg cagctgggtg aaagcaatga agatgcagtt attcgtcgtt gtcgttatga
      301 actgggtgtt gaaattaccc ctccggaaag catttatccg gattttcgtt atcgtgcaac
      361 cgatccgagc ggtattgttg aaaatgaagt ttgtccggtt tttgcagcac gtaccacctc
      421 agcactgcag attaatgatg atgaagtgat ggattatcag tggtgtgatc tggcagatgt
      481 tctgcatggt attgatgcaa ccccgtgggc ctttagcccg tggatggtta tgcaggcaac
      541 caatcgtgaa gcacgtaaac gtctgagcgc atttacccag ctgaaataag agatccggct
      601 gtggctgctg ccactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      661 gttctgtcga cctcgagtaa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa
      721 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      781 tggctcacct tcgggtgggc ctttctgcgt ttatacctag ggatatattc cgcttcctcg
      841 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
      901 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
      961 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
     1021 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct
     1081 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1141 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1201 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt
     1261 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga
     1321 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt
     1381 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac
     1441 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag
     1501 accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt
     1561 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgttact
     1621 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1681 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctctcgaa
     1741 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1801 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1861 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1921 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1981 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     2041 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     2101 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     2161 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     2221 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     2281 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     2341 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     2401 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2461 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2521 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2581 gatcatgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
     2641 cagggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt cgacaccatc
     2701 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     2761 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     2821 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     2881 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     2941 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     3001 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     3061 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     3121 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     3181 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     3241 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     3301 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     3361 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     3421 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     3481 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     3541 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     3601 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     3661 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     3721 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     3781 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     3841 agcgggcagt gagcgcaacg caattaatgt aagttagcgc gaattgatct ggtttgacag
     3901 cttatcatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt
     3961 ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg
     4021 ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag
     4081 ctgttgacaa ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     4141 cagaattcaa aagatctttt aaggacgaaa cgtacatatg aaaaccgtgg tgattattga
     4201 tgcactgcgt accccgattg gtaaatacaa aggtagcctg agccaggtta gcgcagttga
     4261 tctgggcacc catgttacca cccagctgct gaaacgtcat agcaccatta gcgaagaaat
     4321 tgatcaggtg atttttggca atgttctgca ggcaggtaat ggtcagaatc cggcacgtca
     4381 gattgcaatt aatagcggtc tgagccatga aattccggca atgaccgtta atgaagtttg
     4441 tggtagcggt atgaaagcag ttattctggc aaaacagctg atccagctgg gcgaagccga
     4501 agttctgatt gccggtggta ttgaaaatat gagccaggca ccgaaactgc agcgtttcaa
     4561 ttatgaaacc gaaagctatg atgcaccgtt tagcagcatg atgtatgatg gtctgaccga
     4621 tgcatttagc ggtcaggcaa tgggtctgac agcagaaaat gttgcagaaa aatatcatgt
     4681 gacccgtgaa gaacaggatc agtttagcgt tcatagccag ctgaaagcag cacaggcaca
     4741 ggccgaaggt attttcgcag atgaaattgc accgctggaa gttagcggca ccctggttga
     4801 aaaagatgaa ggtattcgtc cgaatagcag cgttgaaaaa ctgggtacac tgaaaacggt
     4861 gtttaaagaa gatggcaccg ttaccgcagg caatgcaagt accattaatg atggtgcaag
     4921 cgcactgatt attgccagcc aagaatatgc cgaagcacat ggtctgccgt atctggcaat
     4981 tattcgtgat agcgttgaag ttggtattga tccggcatat atgggtatta gcccgattaa
     5041 agcaattcag aaactgctgg cacgtaatca gctgaccacc gaagaaatcg acctgtacga
     5101 aattaatgaa gcatttgccg caaccagcat tgttgttcag cgtgaactgg cactgccgga
     5161 agaaaaagtt aacatttatg gcggtggcat cagcctgggt catgcaattg gtgcaaccgg
     5221 tgcacgtctg ctgaccagcc tgagctatca gctgaatcag aaagagaaaa aatacggcgt
     5281 tgcaagcctg tgtattggtg gtggcctggg tctggcaatg ctgctggaac gccctcaaca
     5341 gaaaaaaaac agccgttttt atcagatgag tccggaagaa cgtctggcca gcctgctgaa
     5401 tgaaggtcag attagcgcag ataccaaaaa agaatttgaa aacaccgcac tgagcagcca
     5461 gattgccaac cacatgattg aaaatcagat cagcgaaacc gaagtgccga tgggtgttgg
     5521 tctgcatctg accgtggatg aaacggatta tctggttccg atggcaaccg aagaaccgag
     5581 cgttattgca gccctgagca atggtgcaaa aattgcacag ggctttaaaa ccgtgaatca
     5641 gcagcgtctg atgcgtggtc agattgtttt ttatgatgtt gccgatgcag aaagcctgat
     5701 tgatgaactg caggttcgtg aaacagaaat tttccagcag gcagaactga gttatccgag
     5761 cattgttaaa cgcggtggtg gtctgcgtga tctgcagtat cgtgcatttg atgaaagttt
     5821 tgttagcgtg gattttctgg tggatgttaa agacgcaatg ggtgccaata ttgttaatgc
     5881 aatgctggaa ggtgttgccg aactgtttcg tgaatggttt gcagaacaaa aaatcctgtt
     5941 tagcatcctg agtaactatg ccaccgaaag cgttgttacc atgaaaacag caattccggt
     6001 tagccgtctg agcaaaggta gtaatggtcg tgaaattgcc gaaaaaattg ttctggcaag
     6061 ccgttatgcc agcctggatc cgtatcgtgc cgttacccat aataaaggta ttatgaatgg
     6121 cattgaagca gttgtgctgg ccaccggtaa tgatacccgt gcagttagcg caagctgtca
     6181 tgcatttgca gttaaagaag gtcgttatca gggtctgacc agctggaccc tggatggtga
     6241 gcagctgatt ggtgaaatta gcgttccgct ggcactggca accgttggtg gtgccaccaa
     6301 agttctgccg aaaagccagg cagcagccga tctgctggca gttaccgatg caaaagaact
     6361 gagccgtgtt gttgcagcag ttggtctggc acagaatctg gcagcactgc gtgcactggt
     6421 tagcgaaggc attcagaaag gtcacatggc actgcaggca cgttcactgg ccatgaccgt
     6481 gggtgcgacc ggtaaagaag ttgaagccgt tgcacagcaa ctgaaacgcc agaaaacaat
     6541 gaatcaggat cgtgccctgg caattctgaa tgatctgcgt aaacagtaat gattagcgac
     6601 aaaatatgag gagtgcaaaa aatgaccatt ggcatcgaca aaatcagctt ttttgttccg
     6661 ccttactata tcgacatgac cgcactggcc gaagcacgta atgttgatcc gggtaaattt
     6721 catattggta ttggtcagga tcagatggcc gttaatccga ttagccagga tattgttacc
     6781 tttgcagcaa atgcagcaga agcaattctg accaaagaag ataaagaagc catcgatatg
     6841 gttattgttg gcaccgaaag cagcattgat gaaagcaaag cagccgcagt tgttctgcat
     6901 cgtctgatgg gtattcagcc gtttgcacgt agctttgaaa ttaaagaagg ttgttacggc
     6961 gcaaccgcag gtctgcagct ggcaaaaaat catgttgcac tgcatccgga taaaaaagtt
     7021 ctggttgttg cagcagatat cgccaaatat ggtctgaata gcggtggtga accgacccag
     7081 ggtgccggtg cagttgcaat gctggttgca agcgaaccgc gtattctggc actgaaagag
     7141 gataatgtta tgctgacgca ggatatctat gatttttggc gtccgaccgg tcatccgtat
     7201 ccgatggttg atggtccgct gagcaatgaa acctatattc agagctttgc acaggtgtgg
     7261 gatgaacata aaaaacgtac cggtctggat ttcgcagatt atgatgcact ggcctttcat
     7321 attccgtata ccaaaatggg taaaaaagca ctgctggcga aaattagcga tcagaccgaa
     7381 gccgaacaag aacgtatcct ggcacgttat gaagaaagca ttatctatag ccgtcgtgtg
     7441 ggtaatctgt ataccggtag cctgtatctg ggtctgatta gcctgctgga aaatgcaacc
     7501 accctgaccg ctggtaatca gattggtctg tttagctatg gtagcggtgc cgttgcagaa
     7561 ttctttaccg gtgaactggt tgcaggttat cagaatcatc tgcagaaaga aacccatctg
     7621 gccctgctgg ataatcgtac cgaactgagc attgcagaat atgaagcaat gtttgcagaa
     7681 accctggata ccgatattga tcagaccctg gaagacgaat taaaatatag cattagcgcc
     7741 attaataaca ccgtgcgtag ctatcgtaat taaagctgtt gacaattaat catccggctc
     7801 gtataatgtg tggaattgtg agcggataac aatttcagac aggctcccat ttaacacgtg
     7861 aggaggcata cgatgaccaa aaaagttggt gttggtcagg cacatagcaa aattatcctg
     7921 attggtgaac atgccgtggt ttatggttat ccggcaatta gcctgccgct gctggaagtt
     7981 gaagttacct gtaaagttgt tagcgcagaa agcccgtggc gtctgtatga agaggatacc
     8041 ctgagcatgg cagtttatgc aagcctggaa tatctggata tcaccgaagc atgtgttcgt
     8101 tgtgaaattg atagcgcaat tccggaaaaa cgtggtatgg gtagcagcgc agcaattagc
     8161 attgcagcaa ttcgtgcagt gttcgattat tatcaggccg atctgccgca tgatgttctg
     8221 gaaattctgg ttaatcgtgc agaaatgatt gcccacatga atccgagcgg tctggatgca
     8281 aaaacctgtc tgagcgatca gccgattcgt tttatcaaaa atgtgggttt taccgaactg
     8341 gaaatggatc tgagcgcata tctggttatt gcagataccg gtgtttatgg tcatacccgt
     8401 gaagcaattc aggttgttca gaataaaggt aaagatgcac tgccgtttct gcatgcactg
     8461 ggtgaactga cccagcaggc agaagttgcc attagccaga aatatgcaga aggtctgggt
     8521 ctgattttta gccaggcaca tctgcatctg aaagaaattg gtgttagcag tccggaagca
     8581 gattttctgg ttgaaaccgc actgagctat ggtgccctgg gtgcaaaaat gagcggtggt
     8641 ggtctgggtg gttgtattat tgcactggtt accaatctga cccatgcaca agaactggca
     8701 gaacgtctgg aagaaaaagg tgccgttcag acctggattg aaagcctgta aacaaactca
     8761 aaggaaacat agaaggggca aaggatgatt gccgttaaaa cctgcggtaa actgtattgg
     8821 gcaggcgaat acgcaattct ggaaccgggt cagctggcac tgattaaaga tattccgatt
     8881 tatatgcgtg ccgaaatcgc atttagcgat agctaccgta tttatagcga tatgtttgat
     8941 tttgccgttg atctgcgtcc gaacccggat tatagcctga ttcaagaaac cattgcactg
     9001 atgggtgatt ttctggcagt tcgtggtcag aacctgcgtc cgtttagcct gaaaatttgt
     9061 ggtaaaatgg aacgcgaggg caaaaaattc ggtctgggta gcagcggtag cgttgttgtt
     9121 ctggttgtta aagcactgct ggccctgtat aatctgagcg ttgatcagaa tctgctgttt
     9181 aaactgacca gcgcagttct gctgaaacgt ggtgataatg gtagcatggg tgatctggca
     9241 tgtattgttg cagaagatct ggttctgtat cagagctttg atcgtcagaa agcagcagca
     9301 tggctggaag aagaaaatct ggcaaccgtt ctggaacgtg attggggctt ttttatcagc
     9361 caggttaaac cgaccctgga atgtgatttc ctggttggtt ggaccaaaga agttgcagtt
     9421 agcagccaca tggttcagca gattaaacag aacattaacc agaactttct gtccagcagc
     9481 aaagaaaccg ttgttagcct ggttgaagca ctggaacagg gtaaagcaga aaaagttatt
     9541 gaacaggttg aagtggcaag caaactgctg gaaggtctga gcaccgatat ctatacaccg
     9601 ctgctgcgtc agctgaaaga agcaagccag gatctgcagg cagttgcaaa aagcagtggt
     9661 gccggtggtg gtgattgtgg tattgcactg tcatttgatg cacagagcag ccgtaatacc
     9721 ctgaaaaatc gttgggcaga tctgggtatt gaactgctgt atcaagaacg tattggccac
     9781 gacgataaaa gctaaaaaac actagtccac aattacttaa ggagggtcct atggatcgcg
     9841 aaccggttac cgttcgtagc tatgcaaata ttgccatcat caaatattgg ggcaaaaaaa
     9901 aagaaaaaga gatggttccg gcaaccagca gcattagcct gaccctggaa aatatgtata
     9961 ccgaaaccac cctgagtccg ctgcctgcaa atgttaccgc agatgaattt tacattaatg
    10021 gccagctgca gaacgaagtt gaacatgcaa aaatgagcaa aatcatcgat cgttatcgtc
    10081 cggcaggcga aggttttgtt cgtattgata cccagaataa tatgccgacc gcagcaggtc
    10141 tgagcagcag cagtagcggt ctgagcgcac tggttaaagc atgtaatgca tatttcaaac
    10201 tgggtctgga tcgtagtcag ctggcacaag aagcaaaatt tgcaagcggt agcagcagcc
    10261 gttcatttta tggtccgctg ggtgcatggg ataaagatag cggtgaaatt tatccggttg
    10321 aaaccgatct gaaactggca atgattatgc tggttctgga agataaaaaa aaaccgatta
    10381 gcagccgtga tggtatgaaa ctgtgtgttg aaaccagcac cacctttgat gattgggttc
    10441 gtcagagcga aaaagattat caggatatgc tgatctatct gaaagagaac gatttcgcca
    10501 aaattggtga actgaccgaa aaaaatgccc tggcaatgca tgcaaccacc aaaaccgcaa
    10561 gtccggcatt tagctatctg accgatgcaa gctatgaagc aatggcattt gttcgtcagc
    10621 tgcgtgaaaa aggtgaagca tgttatttta cgatggatgc aggtccgaac gtgaaagttt
    10681 tttgccaaga gaaagatctg gaacacctga gcgaaatttt tggtcagcgt tatcgcctga
    10741 ttgtgagcaa aaccaaagat ctgagccagg atgattgtt
//
