LOCUS       sequence-237232-        9491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-cjBCCIP
FEATURES             Location/Qualifiers
     source          1..9491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             744..2396
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2747..2764)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2753..2977
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3213..3257)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3442..3675
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4168..4293
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4340..4520
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4521..4747)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4775..4795)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4775..4793)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4814..4830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4814..4830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4827..4849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4838..4854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4862..4868,4869..4886,4887..4892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4907..4928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5045..5062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5216..5804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5296..5315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5975..6766,6767..6835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6598..6617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6836..6940)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6966..7421)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7103..7124)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7315..7334
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7548..7570
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7562..7579
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7563..7579
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7589..7608
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7589..7607
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7628..7763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7782..7903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7793..7812)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7847..7866
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7975..8208
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8346..8945)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8429..8449)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8926..8945
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8966..9465)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9014..9033)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9407..9430
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        join(9477..9491,1..737)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
ORIGIN
        1 ctcgcggggg ccgcttaagc ggtggttagg gtttgtctga cgcgggggga gggggaagga
       61 acgaaacact ctcattcgga ggcggctcgg ggtttggtct tggtggccac gggcacgcag
      121 aagagcgccg cgatcctctt aagcaccccc ccgccctccg tggaggcggg ggtttggtcg
      181 gcgggtggta actggcgggc cgctgactcg ggcgggtcgc gcgccccaga gtgtgacctt
      241 ttcggtctgc tcgcagaccc ccgggcggcg ccgccgcggc ggcgacgggc tcgctgggtc
      301 ctaggctcca tggggaccgt atacgtggac aggctctgga gcatccgcac gactgcggtg
      361 atattaccgg agaccttctg cgggacgagc cgggtcacgc ggctgacgcg gagcgtccgt
      421 tgggcgacaa acaccaggac ggggcacagg tacactatct tgtcacccgg aggcgcgagg
      481 gactgcagga gcttcaggga gtggcgcagc tgcttcatcc ccgtggcccg ttgctcgcgt
      541 ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa
      601 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
      661 ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
      721 tgcagcgacc cgcttaagga tccatggaag atgccaaaaa cattaagaag ggcccagcgc
      781 cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct
      841 acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct
      901 acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga
      961 atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt
     1021 tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg
     1081 agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc
     1141 tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg
     1201 atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc
     1261 cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg
     1321 ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc
     1381 gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc
     1441 ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc
     1501 tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat
     1561 tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta
     1621 gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg
     1681 ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc
     1741 taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc
     1801 ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg
     1861 tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc
     1921 gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg
     1981 acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct
     2041 tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg
     2101 aactggagag catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc
     2161 ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca
     2221 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc
     2281 gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc
     2341 gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc gtgtaattct
     2401 agttgtttaa acgtggttca ccggggcaag ggacagccct ggcttctctg aaggctggac
     2461 taattcaagt aagatcaaat ctaagtggtt cccagggaac catcatggct gttggaattg
     2521 ctctgtcgta gtaaggcagg gatatttagg aggctcacag tctcatggag ggataaaaca
     2581 tctcagcact tagtaaccgt aagttaatca acatttggta gtttcccttc ggatcttaaa
     2641 tgctatgatt ttctgaagtt gcagtgtctg tcgtttggag tttgagatac cttgttttca
     2701 ttttaaaaat tttaatctag agtcgacgct agagctcgct gatcagcctc gactgtgcct
     2761 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2821 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2881 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2941 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     3001 tggggctcta gggggtatcc cctcccccct tttcttttgg gtcccctcgg ggttgggagg
     3061 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     3121 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     3181 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     3241 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     3301 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     3361 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     3421 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     3481 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     3541 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     3601 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     3661 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     3721 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     3781 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc
     3841 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     3901 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     3961 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     4021 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
     4081 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     4141 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     4201 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     4261 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     4321 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     4381 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     4441 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     4501 tggtctaacc agagagaccc gtttatgtat cgagctaggc acttaaatac aatatctctg
     4561 caatgcggca attcagtggt tcgtccaatc catgtcagac ccgtctgttg ccttcctaat
     4621 aaggcacgat cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct
     4681 tgtaaggcat gttgctaact catcgttacc atgttgcaag actacaagag tattgcataa
     4741 gactacatta agcttgcagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     4801 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4861 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4921 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4981 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5041 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5101 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5161 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5221 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5281 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5341 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5401 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5461 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5521 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5581 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5641 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5701 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5761 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5821 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5881 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5941 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6001 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6061 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6121 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6181 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6241 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6301 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6361 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6421 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6481 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6541 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6601 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6661 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6721 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6781 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6841 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6901 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6961 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     7021 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7081 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     7141 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     7201 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7261 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     7321 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     7381 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     7441 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     7501 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     7561 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     7621 gtccctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     7681 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggra
     7741 ctgggcggag ttaggggcgg gatagctaga gccagacatg ataagataca ttgatgagtt
     7801 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7861 tattgcttta tttgtaacca ttataagctg caataaacaa gttcctctca ctctctgata
     7921 ttcatttctt tgcaagttat aaatactgaa taataagatg acatgaacta ctactgctag
     7981 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     8041 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     8101 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     8161 aagcaaaaag cagatcttgt cttcgttggg agtgagctag cccttccagt cccccctttt
     8221 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtaccg
     8281 ggccctcgag atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc
     8341 gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg
     8401 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag
     8461 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg
     8521 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac
     8581 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc
     8641 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg
     8701 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg
     8761 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg
     8821 tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg
     8881 gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg
     8941 gtcatggtaa gcttgggctg caggtcgaaa ggcccggaga tgaggaagag gagaacagcg
     9001 cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc
     9061 ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct
     9121 gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct
     9181 tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt
     9241 gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag
     9301 tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg
     9361 tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta
     9421 aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag aattgaaatg
     9481 agtcttcgga c
//
