LOCUS       sequence-237234-        9480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-cjSTK4
FEATURES             Location/Qualifiers
     source          1..9480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..778
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             785..2437
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2777..2794)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2783..3007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3243..3287)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3472..3705
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4198..4323
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4370..4550
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4551..4777)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4805..4825)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4805..4823)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4844..4860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4844..4860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4857..4879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4868..4884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4892..4898,4899..4916,4917..4922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4937..4958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5075..5092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5246..5834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5326..5345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6005..6796,6797..6865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6628..6647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6866..6970)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6996..7451)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7133..7154)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7345..7364
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7578..7600
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7592..7609
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7593..7609
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7619..7638
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7619..7637
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7658..7793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7812..7933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7823..7842)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7877..7896
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8005..8238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8376..8975)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8459..8479)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8956..8975
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(8996..9480,1..15))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9044..9063)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9437..9460
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcgcctcccc tacccggtag aattgaaatg agtcttcgga cctcgcgggg gccgcttaag
       61 cggtggttag ggtttgtctg acgcgggggg agggggaagg aacgaaacac tctcattcgg
      121 aggcggctcg gggtttggtc ttggtggcca cgggcacgca gaagagcgcc gcgatcctct
      181 taagcacccc cccgccctcc gtggaggcgg gggtttggtc ggcgggtggt aactggcggg
      241 ccgctgactc gggcgggtcg cgcgccccag agtgtgacct tttcggtctg ctcgcagacc
      301 cccgggcggc gccgccgcgg cggcgacggg ctcgctgggt cctaggctcc atggggaccg
      361 tatacgtgga caggctctgg agcatccgca cgactgcggt gatattaccg gagaccttct
      421 gcgggacgag ccgggtcacg cggctgacgc ggagcgtccg ttgggcgaca aacaccagga
      481 cggggcacag gtacactatc ttgtcacccg gaggcgcgag ggactgcagg agcttcaggg
      541 agtggcgcag ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg
      601 aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt
      661 cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg tccacttcgc
      721 atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaagg
      781 atccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga
      841 cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac
      901 catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat
      961 gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt
     1021 ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg
     1081 tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg
     1141 catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt
     1201 gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca
     1261 gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta
     1321 cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag
     1381 tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt
     1441 cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctcag
     1501 cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg
     1561 ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga
     1621 ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac
     1681 tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct
     1741 cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg
     1801 ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc
     1861 tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg
     1921 taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag
     1981 cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca
     2041 cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa
     2101 gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct
     2161 gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga
     2221 gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt
     2281 ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt
     2341 ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct
     2401 cattaaggcc aagaagggcg gcaagatcgc cgtgtaattc tagttgttta aacgcaaggc
     2461 caggctgtga gggcccagct ccacccaggc tgtggtgaat tctggatggc ttgcctcaca
     2521 tttgttagcc agcacttctg ctctgtcatt tctcctttgt gaactcagga atgtgcgcca
     2581 gtgggaaggg ctctcttgac agtcaacgtg ccatcttgat gtgtgtatgt acattggtca
     2641 ggtatattat ttcaaaggat ttatattggc gctttcaact cagagtttta aaccccagga
     2701 acagagactc ctagttgagt gatagctggg aaagttttac attgttctag agtcgacgct
     2761 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2821 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2881 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2941 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3001 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc cctcccccct
     3061 tttcttttgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     3121 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     3181 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     3241 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     3301 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     3361 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     3421 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     3481 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     3541 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     3601 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     3661 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     3721 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     3781 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
     3841 ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc
     3901 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
     3961 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
     4021 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc
     4081 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
     4141 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
     4201 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
     4261 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
     4321 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
     4381 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     4441 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     4501 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc gtttatgtat
     4561 cgagctaggc acttaaatac aatatctctg caatgcggca attcagtggt tcgtccaatc
     4621 catgtcagac ccgtctgttg ccttcctaat aaggcacgat cgtaccacct tacttccacc
     4681 aatcggcatg cacggtgctt tttctctcct tgtaaggcat gttgctaact catcgttacc
     4741 atgttgcaag actacaagag tattgcataa gactacatta agcttgcagc tccagctttt
     4801 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     4861 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4921 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4981 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5041 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5101 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5161 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5221 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5281 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5341 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5401 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5461 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5521 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5581 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5641 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5701 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5761 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5821 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5881 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5941 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6001 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6061 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6121 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6181 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6241 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6301 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6361 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6421 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6481 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6541 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6601 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6661 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6721 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6781 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6841 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6901 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6961 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     7021 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7081 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7141 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7201 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7261 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7321 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7381 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7441 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     7501 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     7561 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     7621 atacgactca ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac
     7681 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     7741 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatagctaga
     7801 gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7861 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7921 caataaacaa gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa
     7981 taataagatg acatgaacta ctactgctag agattttcca cactgactaa aagggtctga
     8041 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     8101 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     8161 agatctggtc taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg
     8221 agtgagctag cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg
     8281 ccttgtaagt cattggtctt aaaggtaccg ggccctcgag atgcatgggg tcgtgcgctc
     8341 ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg
     8401 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
     8461 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc
     8521 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
     8581 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
     8641 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
     8701 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
     8761 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
     8821 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg
     8881 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg
     8941 gtggcgaggc gcaccgtggg cttgtactcg gtcatggtaa gcttgggctg caggtcgaaa
     9001 ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga agcgtgcaga
     9061 atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc
     9121 cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag caaagctgct
     9181 attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg tccatctgca
     9241 cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg agctgcgggg
     9301 cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga
     9361 acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag aggccacttg
     9421 tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc cttgggaaaa
//
