LOCUS       sequence-237240-        5418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-DDR1
FEATURES             Location/Qualifiers
     source          1..5418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(919..946)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1038..1124)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1173..1195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1187..1204
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1188..1204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1220..1319
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3948..4047)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4064..4083)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4065..4083)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4088..4104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4088..4104)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4535..5326
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      901 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      961 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     1021 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1081 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     1141 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     1201 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
     1261 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcac
     1321 catgggacca gaggccctgt catctttact gctgctgctc ttggtggcaa gtggagatgc
     1381 tgacatgaag ggacattttg atcctgccaa gtgccgctat gccctgggca tgcaggaccg
     1441 gaccatccca gacagtgaca tctctgcttc cagctcctgg tcagattcca ctgccgcccg
     1501 ccacagcagg ttggagagca gtgacgggga tggggcctgg tgccccgcag ggtcggtgtt
     1561 tcccaaggag gaggagtact tgcaggtgga tctacaacga ctgcacctgg tggctctggt
     1621 gggcacccag ggacggcatg ccgggggcct gggcaaggag ttctcccgga gctaccggct
     1681 gcgttactcc cgggatggtc gccgctggat gggctggaag gaccgctggg gtcaggaggt
     1741 gatctcaggc aatgaggacc ctgagggagt ggtgctgaag gaccttgggc cccccatggt
     1801 tgcccgactg gttcgcttct acccccgggc tgaccgggtc atgagcgtct gtctgcgggt
     1861 agagctctat ggctgcctct ggagggatgg actcctgtct tacactgccc ctgtggggca
     1921 gacaatgtat ttatctgagg ccgtgtacct caacgactcc acctatgacg gacataccgt
     1981 gggcggactg cagtatgggg gtctgggcca gctggcagat ggtgtggtgg ggctggatga
     2041 ctttaggaag agtcaggagc tgcgggtctg gccaggctat gactatgtgg gatggagcaa
     2101 ccacagcttc tccagtggct atgtggagat ggagtttgag tttgaccggc tgagggcctt
     2161 ccaggctatg caggtccact gtaacaacat gcacacgctg ggagcccgtc tgcctggcgg
     2221 ggtggaatgt cgcttccggc gtggccctgc catggcctgg gagggggagc ccatgcgcca
     2281 caacctaggg ggcaacctgg gggaccccag agcccgggct gtctcagtgc cccttggcgg
     2341 ccgtgtggct cgctttctgc agtgccgctt cctctttgcg gggccctggt tactcttcag
     2401 cgaaatctcc ttcatctctg atgtggtgaa caattcctct ccggcactgg gaggcacctt
     2461 cccgccagcc ccctggtggc cgcctggccc acctcccacc aacttcagca gcttggagct
     2521 ggagcccaga ggccagcagc ccgtggccaa ggccgagggg agcccgaccg ccatcctcat
     2581 cggctgcctg gtggccatca tcctgctcct gctgctcatc attgccctca tgctctggcg
     2641 gctgcactgg cgcaggctcc tcagcaaggc tgaacggagg gtgttggaag aggagctgac
     2701 ggttcacctc tctgtccctg gggacactat cctcatcaac aaccgcccag gtcctagaga
     2761 gccacccccg taccaggagc cccggcctcg tgggaatccg ccccactccg ctccctgtgt
     2821 ccccaatggc tctgcctaca gtggggacta tatggagcct gagaagccag gcgccccgct
     2881 tctgccccca cctccccaga acagcgtccc ccattatgcc gaggctgaca ttgttaccct
     2941 gcagggcgtc accgggggca acacctatgc tgtgcctgca ctgcccccag gggcagtcgg
     3001 ggatgggccc cccagagtgg atttccctcg atctcgactc cgcttcaagg agaagcttgg
     3061 cgagggccag tttggggagg tgcacctgtg tgaggtcgac agccctcaag atctggttag
     3121 tcttgatttc ccccttaatg tgcgtaaggg acaccctttg ctggtagctg tcaagatctt
     3181 acggccagat gccaccaaga atgccaggaa tgatttcctg aaagaggtga agatcatgtc
     3241 gaggctcaag gacccaaaca tcattcggct gctgggcgtg tgtgtgcagg acgaccccct
     3301 ctgcatgatt actgactaca tggagaacgg cgacctcaac cagttcctca gtgcccacca
     3361 gctggaggac aaggcagccg agggggcccc tggggacggg caggctgcgc aggggcccac
     3421 catcagctac ccaatgctgc tgcatgtggc agcccagatc gcctccggca tgcgctatct
     3481 ggccacactc aactttgtac atcgggacct ggccacgcgg aactgcctag ttggggaaaa
     3541 tttcaccatc aaaatcgcag actttggcat gagccggaac ctctatgctg gggactatta
     3601 ccgtgtgcag ggccgggcag tgctgcccat ccgctggatg gcctgggagt gcatcctcat
     3661 ggggaagttc acgactgcga gtgacgtgtg ggcctttggt gtgaccctgt gggaggtgct
     3721 gatgctctgt agggcccagc cctttgggca gctcaccgac gagcaggtca tcgagaacgc
     3781 gggggagttc ttccgggacc agggccggca ggtgtacctg tcccggccgc ctgcctgccc
     3841 gcagggccta tatgagctga tgcttcggtg ctggagccgg gagtctgagc agcgaccacc
     3901 cttttcccag ctgcatcggt tcctggcaga ggatgcactc aacacggtgc caactttctt
     3961 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     4021 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     4081 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     4141 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     4201 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     4261 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     4321 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     4381 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     4441 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     4501 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     4561 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     4621 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     4681 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     4741 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     4801 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     4861 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     4921 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     4981 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     5041 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     5101 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     5161 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     5221 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     5281 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     5341 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     5401 aaagatcaaa ggatcttc
//
