LOCUS       sequence-237305-        5045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FMN-based fluorescent protein Reporter plasmid for B-Rex contain 
            perfect operator sequence (anaerobic purpose).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Liu Y, Landick R, Raman S
  TITLE     A Regulatory NADH/NAD+ Redox Biosensor for Bacteria.
  JOURNAL   ACS Synth Biol. 2019 Feb 15;8(2):264-273. doi: 
            10.1021/acssynbio.8b00485. Epub 2019 Jan 23.
  PUBMED    30633862
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00485"; journalName: "ACS Synth Biol"; date: 
            "2019-02-15- 15"; volume: "8"; issue: "2"; pages: "264-273"
COMMENT     Sequence Label: pROP-PP-FBFP
FEATURES             Location/Qualifiers
     source          1..5045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..30
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     promoter        123..157
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     terminator      977..1071
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1563..1785
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1833..2783
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             3360..4151
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVITEVSTQLSEVVGAIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(4256..4342)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4957..4975)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggctcacctt cacgggtggg cctttcttcg gcacgggcaa attgctgaat attccttttc
       61 ttagacgtca ggtggcaaac gcgaagtaat cttttcggtt ttaaagaaaa agggggcgcg
      121 cctttacggc tagctcagcc ctaggtatta tgctagcatg gagcaagaag gtaagcgagg
      181 ataagaagga aaagaatggt ttccaagggc gaggaggata acatggctat cattaaagag
      241 ttcatgcgct tcaaagttca catggagggt tctgttaacg gtcacgagtt cgagatcgaa
      301 ggcgaaggcg agggccgtcc gtatgaaggc acccagaccg ccaaactgaa agtgactaaa
      361 ggcggcccgc tgccttttgc gtgggacatc ctgagcccgc aatttatgta cggttctaaa
      421 gcgtatgtta aacacccagc ggatatcccg gactatctga agctgtcttt tccggaaggt
      481 ttcaagtggg aacgcgtaat gaattttgaa gatggtggtg tcgtgaccgt cactcaggac
      541 tcctccctgc aggatggcga gttcatctat aaagttaaac tgcgtggtac taattttcca
      601 tctgatggcc cggtgatgca gaaaaagacg atgggttggg aggcgtctag cgaacgcatg
      661 tatccggaag atggtgcgct gaaaggcgaa attaaacagc gcctgaaact gaaagatggc
      721 ggccattatg acgctgaagt gaaaaccacg tacaaagcca agaaacctgt gcagctgcct
      781 ggcgcgtaca atgtgaatat taaactggac atcacctctc ataatgaaga ttatacgatc
      841 gtagagcaat atgagcgcgc ggagggtcgt cattctaccg gtggcatgga tgagctgtaa
      901 aataacaggg tggtgacacc ttgcccgttt ttttgccgga gctagagctg tcagaccaag
      961 tttacgagct cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
     1021 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagcact
     1081 agggacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc
     1141 agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag
     1201 gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc
     1261 tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa
     1321 aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg
     1381 aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca
     1441 gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt
     1501 gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta
     1561 gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc
     1621 tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta
     1681 cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac
     1741 ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata
     1801 gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa
     1861 atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct
     1921 gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt
     1981 tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat
     2041 tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta
     2101 aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat
     2161 tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata
     2221 atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat
     2281 taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat
     2341 ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg
     2401 ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata
     2461 agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc
     2521 tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca
     2581 ttacatcaga ttcctaccta cataacggac taagaaaaac actacacgat gctttaactg
     2641 caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagtatg
     2701 atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac
     2761 tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag tgaagcatca
     2821 agactaacaa acaaaagtag aacaactgtt caccgttaca tatcaaaggg aaaactgtcc
     2881 atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt acgagttttt
     2941 ggtgcattca aagctgttca ccatgaacag atcgacaatg taactactag agctgatcct
     3001 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat
     3061 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     3121 acagtaatac aaggggtgtt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     3181 gcggtggtaa cggtgcagtg gcggttttca tggcttgcta tgactgtttt tttggggtac
     3241 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc catgggtcga tgtttgatgt
     3301 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     3361 tgagggaagc ggtgatcacc gaagtatcga ctcaactatc agaggtagtt ggcgccatcg
     3421 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     3481 gcctgaagcc acacagtgat attgatttgc tagttacggt gaccgtaagg cttgatgaaa
     3541 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     3601 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     3661 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     3721 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     3781 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     3841 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     3901 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     3961 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccaatatc
     4021 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     4081 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     4141 tcggcaaata ataatactag cagaaatcat ccttagcgaa agctaaggat tttttttatc
     4201 tgattaccgc ctttgagtga gccccaatga taacccccta ggtctagggc ggcggatttg
     4261 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
     4321 actgagcctt tcgttttatt tgatgcctct agcacgcgta ccatgggatc ccccgggctg
     4381 caggaattcg atatcaagct tttactccaa cagcttctcg tactccttct gtttggtgat
     4441 gtcgttctga ataccaacaa agtacgtctt atcctcaatc tccatcgggt caatgttcag
     4501 ttcattccag aacatcgtgc cgtctttttt atagttctgg atctggaccg taaccggttc
     4561 tttattttgc agcgcggtgc gaatgttatc gacctctgcc gggtcggtgt gtttgccttg
     4621 caggaagcgt gcatttttgc ctaagatttc ttccgtttcg tagccggtca tttggacgaa
     4681 gccctgattc acatacacaa tcgggttatc ttccagggcc ggatcggtga taacgacgcc
     4741 aacgcgcaca tgatccagcg ccttcttaat cacctccagc tgacccggaa taccaaaaga
     4801 ctgaaaggac gccatgctat aaacctccta atagatttta taagaattgt gaattctttt
     4861 tgtgaagtat ttcacatggc tgtaataata cctaggactg agctagctgt aaatactctt
     4921 tttttgctag catctcgagg tgaagacgaa aaaagggcct cgtgatacgc ctatttttat
     4981 aggttaatgt catgataata atggtttctt agacgtcgga attgttacgt agcaatcaac
     5041 tcact
//
