LOCUS       sequence-237347-        9495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized Pan-Neuronal tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS1-rab-3P::his-24::tagBFP
FEATURES             Location/Qualifiers
     source          1..9495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7307..7327)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7307..7325)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7346..7362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7346..7362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7359..7381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7370..7386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7394..7400,7401..7418,7419..7424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7439..7460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7577..7594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7748..8336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7828..7847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8507..9298,9299..9367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9130..9149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9368..9472)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag gcgagttttg actggctttc aaaaactgag
      721 tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag acatttttgc
      781 aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct ataatagttt
      841 ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt gaaagtatgc
      901 agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata taatctaggt
      961 taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc tttcaaatgg
     1021 tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc ggatttattc
     1081 caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt gaaaaaatga
     1141 gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg agggttccgt
     1201 gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag ccacactgtt
     1261 ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag atgctttcat
     1321 tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa ttatgcatga
     1381 gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg tgtggtttaa
     1441 tctgtgaaag ttttgaatct tctgtttgaa aaaaaaacat ttttaatttg tatatacgag
     1501 tgaattggtt aggccctggt tacgttttcc agcgaattca ttcaaatttc ttattttttc
     1561 tattttttca attaaataat ttagttctct atcacatgtg tatgatcaaa atttcattcc
     1621 taatcgtaag caaatttagg ctcatccgta gtataatttt gttatgaagg gcttcacagt
     1681 aaaagctcaa actccaacat ttagcaacta gcaaccaaca aaaaaccaaa gaaatgtcag
     1741 ttttgaaaag tcgttatttc tttgtcttct cacaattcac acatcatctt ctcgttttcc
     1801 tccgtttttt tgtcgtgctt ccttcttttc tttacacatt tttctaacct ttttttaagt
     1861 ctatcctctc ctaatccttt gtactctcta atcagttttg ctggttgtgc tgcacaattt
     1921 ttatcagatt tatgcgcgaa acaaaagcct ctcgcggaga gctccgccaa atccggtctt
     1981 cttttttcct tgcctatcgg atttcacaaa cattcagttt gtcacttgac gatgtgttgg
     2041 aggcattact actaggaagt gttattcata ttttttattc gtttcttttt ggtgactaaa
     2101 cgaaatattc cgaacgtttt cctgatttca ggcggcggaa acgaagaaga ccatgaataa
     2161 tcaacaggct gccattgcat ccgccagaag tcgagtgagt tctttgaaaa tctttattag
     2221 agttatttgt gacaatttgc cacttttttt ttaatgttta atgaatctgt aacgaaatta
     2281 ataccaaggg tgtccaacag aactatctga gcggttattt aatttttaga tagtatattt
     2341 cttccgatcg taacatacat attttcaagc atagtactgt gatttaagtt acagttttcc
     2401 aatcagtctt gattttaata aaaacttttc aactgcaaaa atgcctctga agtatttttc
     2461 tatattttta tagttgaata attttttgat tggcacaaag accgccgaga tatgagccgt
     2521 taaagtaaag ctgcatggat tcaatatggt ttgtatttga ttggagtcac ttacatggaa
     2581 atatttgtat ttaaatctca tattttaaaa caagacaaac accaaattaa atttttatta
     2641 atctgaacat ttttcaatcg atttggtaaa caaattatta gatgtttccc tcaaaaattg
     2701 taacaattcc taaattgtgg cacttataat aacatcgtcc tgaattctca aaaaaaaccg
     2761 aatgctaaga gagatttcgt ttaactggga cctgtaactg aattttcaaa tattggcagt
     2821 ttttgataaa ctgggaggga aatcaaaaac caaaactaca gtacccctcg tgttataata
     2881 aattgtcatt cactcctttt ttgttcccaa catttgctcc catctcctga cgagaaagaa
     2941 caattttatg agcacgggaa cccgtgggcg tccatccgtt cctcctgtgc aatttcagcc
     3001 cccttcctct acattctctg tgttcccgtt tttatcgttt tatgggtatc gggaacggac
     3061 agatcgcttc catttgcttc tattccgtcc tcacaagacg tcccactaac tgattttctt
     3121 ctgatttctt ttcactgaaa atgggaaaga aagaaaaaga cgatgaatcg acgacttctt
     3181 ctcaggagaa ggtgaagaag aaggataagg agaaagttca tctaaggctg aaagataaag
     3241 taaaaaaact tagaaaatct aagtcgattg cagtatcttc tccagctgat tatatgactt
     3301 gtcagtgtga accatgcaaa aaagtagctc tcgaggtaat aatttttaaa ctcaatacgt
     3361 agttaagttt gaactccttt taatttactt atcatctaat ttaccgtcac aatagtactt
     3421 ccattagcaa ttattttaaa attacatgca tagcttcaat gttcgttttt ttgcgttctc
     3481 atatcgtatg ttgtctgcct ctcgaatact gactgtccga ctagaatgtc ccggtaggat
     3541 tactgtacgg tcactgggaa taaactgttt ctgaaatcaa tctgaaattc cgtagatctt
     3601 cttggttcaa tttcttattc ctcaatcttt ggacaacgtc accttcagag tcgtcgttga
     3661 gtatcccatc attaactagt caatctttct ctctttttca tttcccccaa cttttctgca
     3721 tattttttga cgacgacgac ctcgacggca acgagaatac atttatctat tttttagtat
     3781 agcatatata cattttctac ttttctctaa cccatttcag acgtatagat tttggttttc
     3841 gaatgcaact ttgagatttt tcaaagatct tcagatggga gcagtggact gtctatttga
     3901 tattcttaaa atgtactcta taaaagatga aactgtaatg accaattccc tacataaata
     3961 tttttaacta attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa
     4021 taatgatata tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt
     4081 gaaaaagatt ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat
     4141 tattgactat ttatatatat tatatattat atattttcat ggtttaatca aaatctatta
     4201 aattatttct aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt
     4261 tcaattatct aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa
     4321 aaagagagtg aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg
     4381 cacccaagag agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc
     4441 agaagtgtat agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt
     4501 atgcatcggg cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat
     4561 ttttgtgagc acacacagag agagactcaa acacactcac agcttcacac tgagctccaa
     4621 gagctctgta gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc
     4681 tgcttttttc tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa
     4741 agagttttag agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt
     4801 gctattttaa tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa
     4861 attggaaaat ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta
     4921 tttccttttt tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct
     4981 atgaactttg aatttttgat attttgattc tataaataca taaagtaatc taaatgctct
     5041 tttaaaataa atctacagta gccctatttt cagcctagga tgtctgattc cgctgttgtt
     5101 gccgccgctg tcgagccaaa ggtcccaaag gctaaggccg ccaaggccgc caagccagcc
     5161 gccaaggccg ccaagccaac caaagttgcc aaggccaagg ctccagtcgc tcacccacca
     5221 tacatcaaca tgatcaagga ggccatcaag cagctcaagg accgcaaagg agcttccaag
     5281 caggcaatcc tcaagttcat ctcccaaaac tacaagctcg gagacaatgt catccagatc
     5341 aatgctcatc tccgtcaggc tctcaagcgt ggtgtcacca gcaaggccct tgttcaagcc
     5401 gccggatccg gagccaacgg acgtttccgt gtgccagaga aggccgccgc cgccaagaag
     5461 ccagcagcag ccaagaagcc agcagcagcc aagaaaccag cagccgccaa gaaagccact
     5521 ggagagaaga aggcaaagaa gccagccgct gccaagccaa agaaggccgc caccggagat
     5581 aagaaggtca agaaggccaa gagcccaaag aaggttgcca agccagctgc caagaaggtc
     5641 gccaagtctc cagccaagaa ggccgctcca aagaagatcg ccaagccagc tgccaagaag
     5701 gccgccaagc cagccgccaa ggccgcatgc atgtccgaac tcatcaagga gaacatgcac
     5761 atgaagctct acatggaggg aaccgtagac aaccaccact tcaagtgcac ctccgaggga
     5821 gagggaaagc catacgaggg aacccaaacc atgcgtatca aggtcgtcga gggaggacca
     5881 ctcccattcg ccttcgacat cctcgccacc tccttcctct acgggtccaa ggtaagttta
     5941 aacatatata tactaactaa ccctgattat ttaaattttc agaccttcat caaccacacc
     6001 caaggaatcc cagacttctt caagcaatcc ttcccagagg gattcacctg ggagcgtgtc
     6061 accacctacg aggacggagg agtcctcacc gccacccaag acacctccct ccaagacgga
     6121 tgcctcatct acaacgtcaa gatccgtgga gtcaacttca cctccaacgg accagtcatg
     6181 caaaagaaga ccctcggatg ggagaccttc accgagaccc tctacccagc cgacggagga
     6241 ctagagggac gtaacgacat ggccctcaag ctcgtcggag gctcccacct catcgccaac
     6301 atcaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagacc
     6361 acctaccgtt ccaagaagcc agccaagaac ctcaagatgc caggagtcta ctacgttgac
     6421 taccgtctag agcgtatcaa ggaggccaac aacgagacct acgtcgagca acacgaggtc
     6481 gccgtcgccc gttactgcga cctcccatcc aagctcggac acaagctcaa ctaaaagctt
     6541 tggaattcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     6601 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaaacttctt
     6661 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     6721 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     6781 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgtttttttt
     6841 acttctgata aatttttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     6901 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     6961 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     7021 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt gggggtttcc cctattgttt
     7081 gtcaagagtt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
     7141 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
     7201 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
     7261 aatataccaa acataactgt ttgggcccgg tacccagctt ttgttccctt tagtgagggt
     7321 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7381 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7441 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7501 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8161 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8821 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9121 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9481 cccgaaaagt gccac
//
