LOCUS       sequence-23738-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mAmetrine fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: pBad-mAmetrine
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             68..90
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             110..127
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             131..163
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             176..196
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(248..269)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gactnctgag cttttttcgc aactctctac tgtttctcca tacccgtttt tttgggctag
       61 aaataatttt gtttaacttt aagaaggaga tatacatatg cggggttctc atcatcatca
      121 tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgagaa
      181 cctgtacttc cagggctcga gcatggtgag caagggcgag gagctgttca ccggggtggt
      241 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgcgcggcga
      301 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca cctccggcaa
      361 gctgcccgtg ccctggccca ccctcgtgac caccctgtct tacggcgtgc agtgcttcgc
      421 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      481 cgtccaggag cgcaccatct ccttcaagga cgacggcagc tacaggaccc gcgccgaggt
      541 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      601 ggacggcaac atcctggggc acaagctgga gtacaacatg aacgtgtggg acgnnaatnt
      661 cacggccgac aagcagaaga acggcatcat ggcgaacttc aagatcgagc acaacgtcga
      721 ggacggcggc gtgcagctcg cnaagcgtac ancnaaacac cccatcggcg acgctcgtgc
      781 tgctgctgaa acactactga ctncaaanan ctgtnaaacc ccnaaancgc a
//
