LOCUS       sequence-237383-        8472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to 
            the VPR transcriptional activator for 3 or 4-component MultiSite 
            Gateway Pro assembly..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, 
            Newgard CB
  TITLE     Creation of versatile cloning platforms for transgene expression and
            dCas9-based epigenome editing.
  JOURNAL   Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 
            10.1093/nar/gky1286.
  PUBMED    30590691
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286."
COMMENT     Sequence Label: KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR
FEATURES             Location/Qualifiers
     source          1..8472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(603..726)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      726..735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             738..758
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             783..4883
                     /codon_start=1
                     /product="catalytically dead Streptococcus pyogenes Cas9 
                     mutant with broad PAM compatibility (Hu et al., 2018)"
                     /label=dxCas9(3.7)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4884..4931
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4962..5111
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5136..5156
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5586..5942
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5961..6530
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     protein_bind    6535..6658
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6713..6732)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6714..6732)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6737..6753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6737..6753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6855..6874)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6866..7675
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6933..6952)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7822..8410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8311..8330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga
      601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
      661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca
      721 aagttgccac catggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag
      781 ccgacaagaa gtactccatt gggctcgcta tcggcacaaa cagcgtcggc tgggccgtca
      841 ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc
      901 acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag
      961 ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct
     1021 acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc
     1081 tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca
     1141 atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga
     1201 agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata
     1261 tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg
     1321 tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga
     1381 tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc
     1441 ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc
     1501 ttatcgccct gtccctcggg ctgaccccca actttaaatc taacttcgac ctggccgaag
     1561 ataccaagct tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc
     1621 agatcggcga ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc
     1681 tgctgagtga tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta
     1741 tgatcaagct ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac
     1801 agcaactgcc tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg
     1861 gatacattga cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg
     1921 aaaaaatgga cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca
     1981 aacagcgcac tttcgacaat ggaatcatcc cccaccagat tcacctgggc gaactgcacg
     2041 ctatcctcag gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg
     2101 agaaaatcct cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca
     2161 gattcgcgtg gatgactcgc aaatcagaag agaccatcac tccctggaac ttcgagaaag
     2221 tcgtggataa gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa
     2281 atctgcctaa cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt
     2341 ataacgagct caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt
     2401 ctggagatca gaagaaagct attgtggacc tcctcttcaa gacgaaccgg aaagttaccg
     2461 tgaaacagct caaagaagac tatttcaaaa agattgaatg tttcgactct gttgaaatca
     2521 gcggagtgga ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca
     2581 ttaaagacaa ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc
     2641 tcacccttac gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc
     2701 atctcttcga cgacaaagtc atgaagcagc tcaagaggcg ccgatataca ggatgggggc
     2761 ggctgtcaag aaaactgatc aatggcatcc gagacaagca gagtggaaag acaatcctgg
     2821 attttcttaa gtccgatgga tttgccaacc ggaacttcat tcagttgatc catgatgact
     2881 ctctcacctt taaggaggac atccagaaag cacaagtttc tggccagggg gacagtcttc
     2941 acgagcacat cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg
     3001 ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta
     3061 tcgagatggc ccgagagaac caaaccaccc agaagggaca gaagaacagt agggaaagga
     3121 tgaagaggat tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag
     3181 ttgaaaacac ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg
     3241 acatgtacgt ggatcaggaa ctggacatca atcggctctc cgactacgac gtggacgcta
     3301 tcgtgcccca gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg
     3361 ataaaaacag agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa
     3421 attattggcg gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga
     3481 ctaaggctga acgaggtggc ctgtctgagt tggataaagc cggtttcatc aaaaggcagc
     3541 ttgttgagac acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca
     3601 ccaagtacga tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta
     3661 agctggtctc agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt
     3721 accaccatgc gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat
     3781 atcccaagct tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa
     3841 tgatcgcaaa gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca
     3901 atattatgaa ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac
     3961 cacttatcga aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg
     4021 cgacagtccg gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac
     4081 agaccggagg tttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg
     4141 cacgcaaaaa agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt
     4201 acagtgtact ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca
     4261 aggaactgct gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact
     4321 ttctcgaggc gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt
     4381 actctctctt tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgtgctgc
     4441 agaaaggtaa cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc
     4501 actatgaaaa gctcaaaggg tctcccgaag ataatgagca gaagcagctg ttcgtggaac
     4561 aacacaaaca ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga
     4621 tcctcgccga cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc
     4681 ccatcaggga gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc
     4741 ctgcagcctt caagtacttc gacactacca tagacagaaa gcggtacacc tctacaaagg
     4801 aggtcctgga cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg
     4861 acctctctca gctcggtgga gacaaacggc cggcagccac gaagaaggcc ggccaggcaa
     4921 aaaagaaaaa gggatccgag gccagcggtt ccggacgggc tgacgcattg gacgattttg
     4981 atctggatat gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg
     5041 atgcccttga tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc
     5101 tggacatgct gattaactct agaagttccg gatctccgaa aaagaaacgc aaagttggta
     5161 gccagtacct gcccgacacc gacgaccggc accggatcga ggaaaagcgg aagcggacct
     5221 acgagacatt caagagcatc atgaagaagt cccccttcag cggccccacc gaccctagac
     5281 ctccacctag aagaatcgcc gtgcccagca gatccagcgc cagcgtgcca aaacctgccc
     5341 cccagcctta ccccttcacc agcagcctga gcaccatcaa ctacgacgag ttccctacca
     5401 tggtgttccc cagcggccag atctctcagg cctctgctct ggctccagcc cctcctcagg
     5461 tgctgcctca ggctcctgct cctgcaccag ctccagccat ggtgtctgca ctggctcagg
     5521 caccagcacc cgtgcctgtg ctggctcctg gacctccaca ggctgtggct ccaccagccc
     5581 ctaaacctac acaggccggc gagggcacac tgtctgaagc tctgctgcag ctgcagttcg
     5641 acgacgagga tctgggagcc ctgctgggaa acagcaccga tcctgccgtg ttcaccgacc
     5701 tggccagcgt ggacaacagc gagttccagc agctgctgaa ccagggcatc cctgtggccc
     5761 ctcacaccac cgagcccatg ctgatggaat accccgaggc catcacccgg ctcgtgacag
     5821 gcgctcagag gcctcctgat ccagctcctg cccctctggg agcaccaggc ctgcctaatg
     5881 gactgctgtc tggcgacgag gacttcagct ctatcgccga tatggatttc tcagccttgc
     5941 tgggctctgg cagcggcagc cgggattcca gggaagggat gtttttgccg aagcctgagg
     6001 ccggctccgc tattagtgac gtgtttgagg gccgcgaggt gtgccagcca aaacgaatcc
     6061 ggccatttca tcctccagga agtccatggg ccaaccgccc actccccgcc agcctcgcac
     6121 caacaccaac cggtccagta catgagccag tcgggtcact gaccccggca ccagtccctc
     6181 agccactgga tccagcgccc gcagtgactc ccgaggccag tcacctgttg gaggatcccg
     6241 atgaagagac gagccaggct gtcaaagccc ttcgggagat ggccgatact gtgattcccc
     6301 agaaggaaga ggctgcaatc tgtggccaaa tggacctttc ccatccgccc ccaaggggcc
     6361 atctggatga gctgacaacc acacttgagt ccatgaccga ggatctgaac ctggactcac
     6421 ccctgacccc ggaattgaac gagattctgg ataccttcct gaacgacgag tgcctcttgc
     6481 atgccatgca tatcagcaca ggactgtcca tcttcgacac atctctgttt ggagcaactt
     6541 tgtataataa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     6601 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa
     6661 tcaactactt agatggtatt agtgacctgt actgcagggc ggccgcgata tcccctatag
     6721 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     6781 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     6841 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     6901 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     6961 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     7021 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     7081 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     7141 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     7201 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     7261 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     7321 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     7381 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     7441 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     7501 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     7561 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     7621 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     7681 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     7741 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     7801 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7861 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7921 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7981 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8041 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8101 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8161 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8221 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8281 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8341 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8401 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8461 tgctcacatg tt
//
