LOCUS       sequence-237385-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the
            LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway 
            Pro assembly..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, 
            Newgard CB
  TITLE     Creation of versatile cloning platforms for transgene expression and
            dCas9-based epigenome editing.
  JOURNAL   Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 
            10.1093/nar/gky1286.
  PUBMED    30590691
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286."
COMMENT     Sequence Label: KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(603..726)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      726..735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             738..758
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             783..4883
                     /codon_start=1
                     /product="catalytically dead Streptococcus pyogenes Cas9 
                     mutant with broad PAM compatibility (Hu et al., 2018)"
                     /label=dxCas9(3.7)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4884..4931
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    7501..7624
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7679..7698)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7680..7698)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7703..7719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7703..7719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7821..7840)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7832..8641
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7899..7918)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8788..9376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9277..9296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga
      601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
      661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca
      721 aagttgccac catggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag
      781 ccgacaagaa gtactccatt gggctcgcta tcggcacaaa cagcgtcggc tgggccgtca
      841 ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc
      901 acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag
      961 ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct
     1021 acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc
     1081 tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca
     1141 atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga
     1201 agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata
     1261 tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg
     1321 tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga
     1381 tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc
     1441 ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc
     1501 ttatcgccct gtccctcggg ctgaccccca actttaaatc taacttcgac ctggccgaag
     1561 ataccaagct tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc
     1621 agatcggcga ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc
     1681 tgctgagtga tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta
     1741 tgatcaagct ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac
     1801 agcaactgcc tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg
     1861 gatacattga cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg
     1921 aaaaaatgga cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca
     1981 aacagcgcac tttcgacaat ggaatcatcc cccaccagat tcacctgggc gaactgcacg
     2041 ctatcctcag gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg
     2101 agaaaatcct cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca
     2161 gattcgcgtg gatgactcgc aaatcagaag agaccatcac tccctggaac ttcgagaaag
     2221 tcgtggataa gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa
     2281 atctgcctaa cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt
     2341 ataacgagct caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt
     2401 ctggagatca gaagaaagct attgtggacc tcctcttcaa gacgaaccgg aaagttaccg
     2461 tgaaacagct caaagaagac tatttcaaaa agattgaatg tttcgactct gttgaaatca
     2521 gcggagtgga ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca
     2581 ttaaagacaa ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc
     2641 tcacccttac gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc
     2701 atctcttcga cgacaaagtc atgaagcagc tcaagaggcg ccgatataca ggatgggggc
     2761 ggctgtcaag aaaactgatc aatggcatcc gagacaagca gagtggaaag acaatcctgg
     2821 attttcttaa gtccgatgga tttgccaacc ggaacttcat tcagttgatc catgatgact
     2881 ctctcacctt taaggaggac atccagaaag cacaagtttc tggccagggg gacagtcttc
     2941 acgagcacat cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg
     3001 ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta
     3061 tcgagatggc ccgagagaac caaaccaccc agaagggaca gaagaacagt agggaaagga
     3121 tgaagaggat tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag
     3181 ttgaaaacac ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg
     3241 acatgtacgt ggatcaggaa ctggacatca atcggctctc cgactacgac gtggacgcta
     3301 tcgtgcccca gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg
     3361 ataaaaacag agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa
     3421 attattggcg gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga
     3481 ctaaggctga acgaggtggc ctgtctgagt tggataaagc cggtttcatc aaaaggcagc
     3541 ttgttgagac acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca
     3601 ccaagtacga tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta
     3661 agctggtctc agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt
     3721 accaccatgc gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat
     3781 atcccaagct tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa
     3841 tgatcgcaaa gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca
     3901 atattatgaa ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac
     3961 cacttatcga aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg
     4021 cgacagtccg gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac
     4081 agaccggagg tttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg
     4141 cacgcaaaaa agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt
     4201 acagtgtact ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca
     4261 aggaactgct gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact
     4321 ttctcgaggc gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt
     4381 actctctctt tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgtgctgc
     4441 agaaaggtaa cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc
     4501 actatgaaaa gctcaaaggg tctcccgaag ataatgagca gaagcagctg ttcgtggaac
     4561 aacacaaaca ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga
     4621 tcctcgccga cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc
     4681 ccatcaggga gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc
     4741 ctgcagcctt caagtacttc gacactacca tagacagaaa gcggtacacc tctacaaagg
     4801 aggtcctgga cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg
     4861 acctctctca gctcggtgga gacaaacggc cggcagccac gaagaaggcc ggccaggcaa
     4921 aaaagaaaaa gggaggagga tctttatctg ggaagaaggc ggcagccgcg gcggcggcgg
     4981 ctgcagcggc agcaaccggg acggaggctg gccctgggac agcaggcggc tccgagaacg
     5041 ggtctgaggt ggccgcgcag cccgcgggcc tgtcgggccc agccgaggtc gggccggggg
     5101 cggtggggga gcgcacaccc cgcaagaaag agcctccgcg ggcctcgccc cccgggggcc
     5161 tggcggaacc gccggggtcc gcagggcctc aggccggccc tactgtcgtg cctgggtctg
     5221 cgacccccat ggaaactgga atagcagaga ctccggaggg gcgtcggacc agccggcgca
     5281 agcgggcgaa ggtagagtac agagagatgg atgaaagctt ggccaacctc tcagaagatg
     5341 agtattattc agaagaagag agaaatgcca aagcagagaa ggaaaagaag cttcccccac
     5401 caccccctca agccccacct gaggaagaaa atgaaagtga gcctgaagaa ccatcgggtg
     5461 tggagggcgc agctttccag agccgacttc ctcatgaccg gatgacttct caagaagcag
     5521 cctgttttcc agatattatc agtggaccac aacagaccca gaaggttttt cttttcatta
     5581 gaaaccgcac actgcagttg tggttggata atccaaagat tcagctgaca tttgaggcta
     5641 ctctccaaca attagaagca ccttataaca gtgatactgt gcttgtccac cgagttcaca
     5701 gttatttaga gcgtcatggt cttatcaact tcggcatcta taagaggata aaacccctac
     5761 caactaaaaa gacaggaaag gtaattatta taggctctgg ggtctcaggc ttggcagcag
     5821 ctcgacagtt acaaagtttt ggaatggatg tcacactttt ggaagccagg gatcgtgtgg
     5881 gtggacgagt tgccacattt cgcaaaggaa actatgtagc tgatcttgga gccatggtgg
     5941 taacaggtct tggagggaat cctatggctg tggtcagcaa acaagtaaat atggaactgg
     6001 ccaagatcaa gcaaaaatgc ccactttatg aagccaacgg acaagctgtt cctaaagaga
     6061 aagatgaaat ggtagagcaa gagtttaacc ggttgctaga agctacatct taccttagtc
     6121 atcaactaga cttcaatgtc ctcaataata agcatgtgtc ccttggccag gcattggaag
     6181 ttgtcattca gttacaagag aagcatgtca aagatgagca gattgaacat tggaagaaga
     6241 tagtgaaaac tcaggaagaa ttgaaagaac ttcttaataa gatggtaaat ttgaaagaga
     6301 aaattaaaga actccatcag caatacaaag aagcatctga agtaaagcca cccagagata
     6361 ttactgccga gttcttagtg aaaagcaaac acagggatct gaccgcccta tgcaaggaat
     6421 atgatgaatt agctgaaaca caaggaaagc tagaagaaaa acttcaggag ttggaagcga
     6481 atcccccaag tgatgtatat ctctcatcaa gagacagaca aatacttgat tggcattttg
     6541 caaatcttga atttgctaat gccacacctc tctcaactct ctcccttaag cactgggatc
     6601 aggatgatga ctttgagttc actggcagcc acctgacagt aaggaatggc tactcgtgtg
     6661 tgcctgtggc tttagcagaa ggcctagaca ttaaactgaa tacagcagtg cgacaggttc
     6721 gctacacggc ttcaggatgt gaagtgatag ctgtgaatac ccgctccacg agtcaaacct
     6781 ttatttataa atgcgacgca gttctctgta cccttcccct gggtgtgctg aagcagcagc
     6841 caccagccgt tcagtttgtg ccacctctcc ctgagtggaa aacatctgca gtccaaagga
     6901 tgggatttgg caaccttaac aaggtggtgt tgtgttttga tcgggtgttc tgggatccaa
     6961 gtgtcaattt gttcgggcat gttggcagta cgactgccag caggggtgag ctcttcctct
     7021 tctggaacct ctataaagct ccaatactgt tggcactagt ggcaggagaa gctgctggta
     7081 tcatggaaaa cataagtgac gatgtgattg ttggccgatg cctggccatt ctcaaaggga
     7141 tttttggtag cagtgcagta cctcagccca aagaaactgt ggtgtctcgt tggcgtgctg
     7201 atccctgggc tcggggctct tattcctatg ttgctgcagg atcatctgga aatgactatg
     7261 atttaatggc tcagccaatc actcctggcc cctcgattcc aggtgcccca cagccgattc
     7321 cacgactctt ctttgcggga gaacatacga tccgtaacta cccagccaca gtgcatggtg
     7381 ctctgctgag tgggctgcga gaagcgggaa gaattgcaga ccagtttttg ggggccatgt
     7441 atacgctgcc tcgccaggcc acaccaggtg ttcctgcaca gcagtcccca agcatgggcg
     7501 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     7561 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     7621 tatgaatcaa ctacttagat ggtattagtg acctgtactg cagggcggcc gcgatatccc
     7681 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     7741 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     7801 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     7861 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     7921 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     7981 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     8041 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     8101 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     8161 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     8221 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     8281 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     8341 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     8401 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     8461 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     8521 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     8581 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     8641 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     8701 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     8761 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8821 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8881 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8941 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9001 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9061 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9121 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9181 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9241 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9301 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9361 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9421 gccttttgct cacatgtt
//
