LOCUS       sequence-237402-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ER specific HaloTag protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Jiang X, Zhang C, Chen J, Choi S, Zhou Y, Zhao M, Song X, Chen X, 
            Maletic-Savatic M, Palzkill TG, Moore D, Wang MC, Wang J
  TITLE     Quantitative Real-Time Imaging of Glutathione with Sub-Cellular 
            Resolution.
  JOURNAL   Antioxid Redox Signal. 2018 Oct 25. doi: 10.1089/ars.2018.7605.
  PUBMED    30358421
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antioxid 
            Redox Signal. 2018 Oct 25. doi: 10.1089/ars.2018.7605."
COMMENT     Sequence Label: Halo-KDEL
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..948
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
ORIGIN
        1 ggtaccgcca ccatgagcgc cctgctgatc ctggctctcg tgggcgctgc cgtggccgga
       61 tccgaaatcg gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc
      121 atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac
      181 ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc
      241 attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc
      301 gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc
      361 ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag
      421 cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg
      481 ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg
      541 atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg
      601 actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca
      661 ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg
      721 gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc
      781 accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac
      841 tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg
      901 atcggcagcg agatcgcgcg ctggctgtct actctggaga tttccggtaa agatgagcta
      961 taagcggccg c
//
