LOCUS       sequence-23741-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Martin VJ, Pitera DJ, Withers ST, Newman JD, Keasling JD
  TITLE     Engineering a mevalonate pathway in Escherichia coli for production 
            of terpenoids.
  JOURNAL   Nat Biotechnol. 2003 Jul . 21(7):796-802.
  PUBMED    12778056
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2003 Jul . 21(7):796-802."
COMMENT     Sequence Label: pMBI
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..33
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgggacggg cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
       61 taggaggaat taaccatgtc attaccgttc ttaacttctg caccgggaaa ggttattatt
      121 tttggtgaac actctgctgt gtacaacaag cctgccgtcg ctgctagtgt gtctgcgttg
      181 agaacctacc tgctaataag cgagtcatct gcaccagata ctattgaatt ggacttcccg
      241 gacattagct ttaatcataa gtggtccatc aatgatttca atgccatcac cgaggatcaa
      301 gtaaactccc aaaaattggc caaggctcaa caagccaccg atggcttgtc tcaggaactc
      361 gttagtcttt tggatccgtt gttagctcaa ctatccgaat ccttccacta ccatgcagcg
      421 ttttgtttcc tgtatatgtt tgtttgccta tgcccccatg ccaagaatat taagttttct
      481 ttaaagtcta ctttacccat cggtgctggg ttgggctcaa gcgcctctat ttctgtatca
      541 ctggccttag ctatggccta cttggggggg ttaataggat ctaatgactt ggaaaagctg
      601 tcagaaaacg ataagcatat agtgaatcaa tgggccttca taggtgaaaa gtgtattcac
      661 ggtaccccct tcaggaatag ataacgctgt ggccacttat ggtaatgccc tgctatttga
      721 aaaagactca cataatggaa cantaancac aaacaattta agttcttaga tgattcccag
      781 ccatnccatg atcctaacta tactagaatc naggnctana a
//
