LOCUS       sequence-237423-        1162 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mitochondria specific HaloTag protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Jiang X, Zhang C, Chen J, Choi S, Zhou Y, Zhao M, Song X, Chen X, 
            Maletic-Savatic M, Palzkill TG, Moore D, Wang MC, Wang J
  TITLE     Quantitative Real-Time Imaging of Glutathione with Sub-Cellular 
            Resolution.
  JOURNAL   Antioxid Redox Signal. 2018 Oct 25. doi: 10.1089/ars.2018.7605.
  PUBMED    30358421
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antioxid 
            Redox Signal. 2018 Oct 25. doi: 10.1089/ars.2018.7605."
COMMENT     Sequence Label: Halo-MTS
FEATURES             Location/Qualifiers
     source          1..1162
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             233..253
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             269..1153
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
ORIGIN
        1 gctagccacc atggcctcca ctcgtgtcct cgcctctcgc ctggcctccc agatggctgc
       61 ttccgccaag gttgcccgcc ctgctgtccg cgttgctcag gtcagcaagc gcaccatcca
      121 gactggctcc cccctccaga ccctcaagcg cacccagatg acctccatcg tcaacgccac
      181 cacccgccag gctttccaga agcgcgccta ctcttccctc gagccaacca ctgaggatct
      241 gtactttcag agcgataacg atggatccga aatcggtact ggctttccat tcgaccccca
      301 ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac
      361 ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc
      421 gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga
      481 caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga
      541 agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt
      601 ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg
      661 ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg
      721 caccaccgac gtcggccgca agctgatcat cgatcagaac gtttttatcg agggtacgct
      781 gccgatgggt gtcgtccgcc cgctgactga agtcgagatg gaccattacc gcgagccgtt
      841 cctgaatcct gttgaccgcg agccactgtg gcgcttccca aacgagctgc caatcgccgg
      901 tgagccagcg aacatcgtcg cgctggtcga agaatacatg gactggctgc accagtcccc
      961 tgtcccgaag ctgctgttct ggggcacccc aggcgttctg atcccaccgg ccgaagccgc
     1021 tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg gtctgaatct
     1081 gctgcaagaa gacaacccgg acctgatcgg cagcgagatc gcgcgctggc tgtctactct
     1141 ggagatttcc ggttaagggc cc
//
