LOCUS       sequence-237424-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses cytosolic specific HaloTag protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Jiang X, Zhang C, Chen J, Choi S, Zhou Y, Zhao M, Song X, Chen X, 
            Maletic-Savatic M, Palzkill TG, Moore D, Wang MC, Wang J
  TITLE     Quantitative Real-Time Imaging of Glutathione with Sub-Cellular 
            Resolution.
  JOURNAL   Antioxid Redox Signal. 2018 Oct 25. doi: 10.1089/ars.2018.7605.
  PUBMED    30358421
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antioxid 
            Redox Signal. 2018 Oct 25. doi: 10.1089/ars.2018.7605."
COMMENT     Sequence Label: Halo-Cyto
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             109..129
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             145..1029
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
ORIGIN
        1 atgaccggat tgcagaagaa gctggaggag ctagagcttg atgactacaa agacgatgac
       61 gacaagctgg acggcggacc tgcaggacga gctctcgagc caaccactga ggatctgtac
      121 tttcagagcg ataacgatgg atccgaaatc ggtactggct ttccattcga cccccattat
      181 gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga tggcacccct
      241 gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat catcccgcat
      301 gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa atccgacaaa
      361 ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt catcgaagcc
      421 ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct gggtttccac
      481 tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt catccgccct
      541 atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc cttccgcacc
      601 accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg tacgctgccg
      661 atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga gccgttcctg
      721 aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat cgccggtgag
      781 ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca gtcccctgtc
      841 ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga agccgctcgc
      901 ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct gaatctgctg
      961 caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc tactctggag
     1021 atttccggt
//
