LOCUS       sequence-237429-        1168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pET28B-PU.1-ETS-H3
FEATURES             Location/Qualifiers
     source          1..1168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             25..47
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             375..392
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             399..416
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             441..458
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(511..529)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      525..572
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      609..1032
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(696..715)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     906..927
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gnnnnnnntc ccctctagaa taattttgtt taactttaag aaggagatat accatgggca
       61 gcaagaagaa gattcgtctg taccaattcc tgctcgattt gctgcggtca ggagatatga
      121 aagactcgat ctggtgggtc gataaagaca aaggtacctt tcaattcagt tctaaacata
      181 aagaggcatt agcgcacagg tggggaattc agaagggcaa tcgcaagaag atgaattatg
      241 agaaactgag ccgcggcctt cgctactatg gcaagactgg tgaagttaag aaagtgaaga
      301 agaaattgac gtatcagttt agcggcgaag tattagggcg tggtgggctg gccgaaagac
      361 gccttccgcc acatctagtg cctcgaggtt ccagatctca tcaccatcac catcactaaa
      421 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      481 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      541 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      601 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      661 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      721 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      781 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      841 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      901 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      961 tgatttataa nnnttttgcc gatttcggcc tattgnnnaa aaatgagctg atttanaaaa
     1021 tttangcgaa ttttanaaat attangctnn atttagnggc acttttcggg gaaangtgcg
     1081 cganccnatt nnnnntttnc nnannnnntn nannnnnnnn cnntcnnnan tnattnctan
     1141 nannncatcg agcatnnaan nnaacnnc
//
