LOCUS       sequence-237450-       10233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-species CRISPR/Cas9 coexpression system, optimized for Golden 
            Gate cloning of gRNA targets.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Rajkumar AS, Varela JA, Juergens H, Daran JG, Morrissey JP
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology.
  JOURNAL   Front Bioeng Biotechnol. 2019 May 7;7:97. doi: 
            10.3389/fbioe.2019.00097. eCollection 2019.
  PUBMED    31134195
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2019-05-7"; volume: "7:97. doi"; pages: 
            " 10.3389/fbioe.2019.00097. eCollection 2019"
COMMENT     Sequence Label: pUCC001
FEATURES             Location/Qualifiers
     source          1..10233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3409..3429
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        4348..5014
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4884..4907
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     ncRNA           5016..5052
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        5073..5148
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           5153..5220
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(5263..5281)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(5715..6506,6507..6575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6338..6357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6576..6680)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6783..7371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7272..7291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gene            7535..9112
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             7883..8911
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             7883..8911
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             9490..9513
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9517..9537
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9538..10233,1..3405)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa
       61 tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat
      121 gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct
      181 aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac tgaaataact
      241 aaggctcccc tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact
      301 cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat
      361 caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat
      421 aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt ggtgaaacta
      481 aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat tacccatcaa
      541 attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta tccattttta
      601 aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt
      661 ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt
      721 accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa
      781 cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg
      841 ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaagga
      901 atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc
      961 aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa
     1021 tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc
     1081 taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa
     1141 gatatcttag aggatattgt tttaacattg accttatttg aagataggga gatgattgag
     1201 gaaagactta aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt
     1261 cgccgttata ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag
     1321 caatctggca aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt
     1381 atgcagctga tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg
     1441 tctggacaag gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt
     1501 aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg
     1561 cataagccag aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc
     1621 cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga attaggaagt
     1681 cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc
     1741 tattatctcc aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta
     1801 agtgattatg atgtcgatca cattgttcca caaagtttcc ttaaagacga ttcaatagac
     1861 aataaggtct taacgcgttc tgataaaaat cgtggtaaat cggataacgt tccaagtgaa
     1921 gaagtagtca aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact
     1981 caacgtaagt ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa
     2041 gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa
     2101 attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt
     2161 aaagtgatta ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat
     2221 aaagtacgtg agattaacaa ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt
     2281 ggaactgctt tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat
     2341 aaagtttatg atgttcgtaa aatgattgct aagtctgagc aagaaatagg caaagcaacc
     2401 gcaaaatatt tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca
     2461 aatggagaga ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc
     2521 tgggataaag ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat
     2581 attgtcaaga aaacagaagt acagacaggc ggattctcca aggagtcaat tttaccaaaa
     2641 agaaattcgg acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt
     2701 tttgatagtc caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa
     2761 tcgaagaagt taaaatccgt taaagagtta ctagggatca caattatgga aagaagttcc
     2821 tttgaaaaaa atccgattga ctttttagaa gctaaaggat ataaggaagt taaaaaagac
     2881 ttaatcatta aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg
     2941 ctggctagtg ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg
     3001 aattttttat atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa
     3061 caaaaacaat tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc
     3121 agtgaatttt ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca
     3181 tataacaaac atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt
     3241 acgttgacga atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt
     3301 aaacgatata cgtctacaaa agaagtttta gatgccactc ttatccatca atccatcact
     3361 ggtctttatg aaacacgcat tgatttgagt cagctaggag gtgaccctcc aaaaaagaag
     3421 agaaaggtct gagcggtttt gtataactaa ataatattgg aaactaaata cgaataccca
     3481 aattttttat ctaaattttg ccgaaagatt aaaatctgca gagatatccg aaacaggtaa
     3541 atggatgttt caatccctgt agtcagtcag gaacccatat tatattacag tattagtcgc
     3601 cgcttaggca cgcctttaat tagcaaaatc aaaccttaag tgcatatgcc gtataaggga
     3661 aactcaaaga actggcatcg caaaaatgaa aacatacgtt gaaactacgg caaaggattg
     3721 gtcagatcgc ttcatacagg gaaagttcgg catcaacatc tttggataat atcagaatga
     3781 gaaagaacag atacgcagta cgttttttgg tgagctcttt gcacttcttt agttctttcc
     3841 atcaatatca gttttttaaa cttttaggac taaaagtgat gtttaacttc aaaatgttta
     3901 aaattttgtt cttcccgacg ttcattaaga atactaatac actttaataa ttattttaat
     3961 attttgttct aaataatgac ttttaattaa aaaagataaa atataaaaac atcataataa
     4021 ctcaccagag gttaagaaca aaaaaacaaa ttagatatct gctaatccaa tatagttaaa
     4081 tcaatctttc cttggtataa tgggtatatt acatatattt caaggaccga cactcctacc
     4141 aaatatctaa aatttaccat attaacataa catgtatata aacgtcaaat cataatcagc
     4201 actaacgtct cacggatcgt atatgccgta gcgacaatct aagaactatg cgaggacacg
     4261 ctagggccaa ggcaaaaaga ttccttgatt acgtaaggga gttagaatca ttttgaataa
     4321 aaaacacgct ttttcagttc gagtttatca ttatcaatac tgccatttca aagaatacgt
     4381 aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag ccttttaatt
     4441 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcgt
     4501 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt
     4561 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcaccaacc
     4621 atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac aaacaggcaa
     4681 aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga tgacacaagg
     4741 caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc ttctgctctc
     4801 tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat tattccccta
     4861 cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct
     4921 taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa acaccaagaa
     4981 cttagtttcg aataaacaca cataaacaaa caaacctgat gagtccgtga ggacgaaacg
     5041 agtaagctcg tccgagacct gcggaggtct ccgttttaga gctagaaata gcaagttaaa
     5101 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttggccggca
     5161 tggtcccagc ctcctcgctg gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac
     5221 cacaggcccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct tacattcacg
     5281 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     5341 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     5401 tctttttttt ctgtacaaac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     5461 gaaggttttg ggacaagctt acgtctcacg gatcgtatat gccgtagcga caatctaaga
     5521 actatgcgag gacacgctag cagctgtatt cacgtagacg gataggtata gccagacatc
     5581 agcagcatac ttcgggaacc gtaggccacc gagcggccgc gattatcaaa aaggatcttc
     5641 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5701 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5761 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5821 ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat
     5881 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5941 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6001 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6061 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6121 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6181 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6241 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6301 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6361 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6421 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6481 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6541 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6601 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6661 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcat gaccaaaatc
     6721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6781 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6841 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6901 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6961 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7021 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7081 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7141 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7201 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7261 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7321 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7381 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7441 gcgttatccc ctgattctgt ggataaccgt gcggactata tgtgaaggca tggctatggc
     7501 acggcagaca ttccgccaga tcatcaatag gcacgacatg gaggcccaga ataccctcct
     7561 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
     7621 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
     7681 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
     7741 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
     7801 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
     7861 atccgaacat aaacaaggat ccatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga
     7921 gaagtttctg atcgaaaagt tcgacagcgt gtccgacctg atgcagctct cggagggcga
     7981 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     8041 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     8101 cccgattccg gaagtgcttg acattgggga atttagcgag agcctgacct attgcatctc
     8161 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     8221 gcaaccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     8281 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     8341 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     8401 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     8461 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     8521 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     8581 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     8641 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     8701 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     8761 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     8821 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     8881 ccccagcact cgtccgaggg caaaggaata actcgaggac aataaaaaga ttcttgtttt
     8941 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     9001 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     9061 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tggagctctc
     9121 gagaaccctc acctttcgag aggacgatgc ccgtgtctaa atgattcgac cagcctaaga
     9181 atgttcaaca atctataatc agtccatagt caacaagagc cctcaaagtt gatatcgttt
     9241 agtgatctcc ctcgagatga gcggaggatg gttgcttttt ggggagccct aagtggtacg
     9301 tgcggcgagc gggatgtttc cccattaggc aaaggacgac cgggtaaccc ctcgagaaaa
     9361 aaaatttttc gccgctaatc tggtgttata taaagctccc cctgctcctg gaattttttt
     9421 ccttgtcaac tcacaccgga aatcaaggca tttcattctg agtagttctt aaaaacataa
     9481 tcaacaatgg attataaaga tgacgatgac aaacctccaa aaaagaagag aaaggtcgat
     9541 aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact
     9601 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt
     9661 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact
     9721 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta
     9781 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa
     9841 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata
     9901 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg
     9961 gtagattcta cttataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt
    10021 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac
    10081 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac
    10141 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta
    10201 gaaaatctca ttgctcagct ccccggtgag aag
//
