LOCUS       sequence-23746-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Pfleger BF, Fawzi NJ, Keasling JD
  TITLE     Optimization of DsRed production in Escherichia coli: effect of 
            ribosome binding site sequestration on translation efficiency.
  JOURNAL   Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8.
  PUBMED    16247774
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2005 Dec 5. 92(5):553-8."
COMMENT     Sequence Label: pBAD-RFP(EC)
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(207..235)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 accncgtgga cagtcgagcg cgccgagggc cgccaccacc tgttcctgta gatctgatcg
       61 ttgcgggcgg ggcgagagtc tcgccccgcc cgcgaccgcg gtgaaaatac gagaatattt
      121 ttgtattgat ctcctaggcg gggtaccgta ttttggatga taacgaggcg caaaaaatga
      181 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      241 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      301 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      361 ctactttctc ttatggtgtt caatgctttt cccgttatcc ggatcatatg aaacggcatg
      421 actttttcaa gagtgccatg cccgaaggtt atgtacagga acgcactata tctttcaaag
      481 atgacgggaa ctacaagacg cgtgctgaag tcaagtttga aggtgatacc cttgttaatc
      541 gtatcgagtt aaaaggtatt gattttaaag aagatggaaa cattctcgga cacaaactgg
      601 agtacaacta taactcacac aatgtataca tcacgggcag acaaacaaaa gaatgnaatc
      661 aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact agcagaccat
      721 tatcaacaaa tactccantg gcgatggccc tgtcctttta ccaacanccn ncnnnnntcc
      781 ncaatnt
//
