LOCUS       sequence-237527-        7800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus delivery for stable expression of Lb crRNA, has LbDR; 
            Cloning vector for expression of LbCas12a crRNA. It contains BsmBI 
            site for cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Breinig M, Schweitzer AY, Herianto AM, Revia S, Schaefer L, Wendler 
            L, Cobos Galvez A, Tschaharganeh DF
  TITLE     Multiplexed orthogonal genome editing and transcriptional activation
            by Cas12a.
  JOURNAL   Nat Methods. 2019 Jan;16(1):51-54. doi: 10.1038/s41592-018-0262-1. 
            Epub 2018 Dec 17.
  PUBMED    30559432
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0262-1"; journalName: "Nat Methods"; date: 
            "2019-01"; volume: "16"; issue: "1"; pages: "51-54"
COMMENT     Sequence Label: pMB30
FEATURES             Location/Qualifiers
     source          1..7800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..18
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     37..57
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        39..57
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        85..311
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             312..492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    539..664
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1157..1390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1575..1619
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1799..1916
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(1991..2239)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2049..2068)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2219..2239)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2273..2484
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2504..2513
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2510..2512,2513..2515,2516..3226)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2510..2512,2513..2515,2516..3226)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2555..2576)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2816..2835)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3163..3184
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3242..3298
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    3737..4325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3790..3810)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4208..4219)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4397..4630
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4708..4829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4745..4764)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4799..4818
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4869..5004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5024..5043)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5025..5043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5053..5070)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5053..5069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5062..5084)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5211..5666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5298..5317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5508..5529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5692..5796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5797..5865,5866..6657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6015..6034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6828..7416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7317..7336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7570..7587
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7704..7725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7740..7745,7746..7763,7764..7770)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7778..7794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7783..7800,1..5)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
       61 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
      121 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
      181 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
      241 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
      301 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      361 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      421 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      481 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      541 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      601 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      661 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
      721 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
      781 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
      841 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
      901 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
      961 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     1021 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     1081 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     1141 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     1201 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     1261 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     1321 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     1381 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     1441 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     1501 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     1561 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     1621 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1681 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1741 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccaacggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcaac
     1921 ttcggaattc gtgtgctcga gcaaaaaaag agacgatata tcgtctccat ctacacttag
     1981 tagaaattac ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact
     2041 ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact
     2101 acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag
     2161 tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa
     2221 tcatgggaaa taggccctcg ggacccctcg agtggctccg gtgcccgtca gtgggcagag
     2281 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     2341 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2401 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2461 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccacca tggtgagcaa
     2521 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     2581 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     2641 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     2701 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     2761 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     2821 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     2881 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     2941 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     3001 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3061 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3121 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3181 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggccg caggcagcgg
     3241 cgccaccaac ttcagcctgc tgaagcaggc cggcgacgtg gaggagaacc ccggccccat
     3301 gaagaccttc aacatctctc agcaggacct tgagctggtg gaagttgcca ctgagaaaat
     3361 caccatgctc tatgaggaca acaagcacca tgttggtgct gccatcagga ccaagacagg
     3421 agaaatcatc tctgctgtcc acattgaagc ctacattggc agggtcactg tgtgtgcaga
     3481 ggccattgcc attgggtctg cagtctccaa tgggcagaag gactttgaca ccattgtggc
     3541 tgtgaggcac ccctacagtg atgaggtgga caggtccatc agagtggtgt ccccctgtgg
     3601 catgtgcagg gagctcatct cagactatgc ccctgattgc tttgttctga ttgagatgaa
     3661 tggcaagctg gtcaagacaa ccattgagga gctgattcca ctgaaataca ccagaaacta
     3721 aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3781 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3841 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3901 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3961 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4021 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4081 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4141 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4201 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4261 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4321 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4381 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4441 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4501 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4561 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4621 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     4681 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     4741 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4801 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     4861 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4921 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4981 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     5041 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5101 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5161 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     5221 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5281 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5341 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5401 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5461 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5521 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5581 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5641 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     5701 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5761 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5821 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5881 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5941 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6001 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6061 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6121 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6181 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6241 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6301 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6361 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6421 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6481 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6541 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6601 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6661 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6721 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6781 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6841 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6901 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6961 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7021 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7081 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7141 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7201 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7261 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7321 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7381 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7441 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7501 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7561 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7621 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7681 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7741 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
//
