LOCUS       sequence-23753-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Pfleger BF, Pitera DJ, Smolke CD, Keasling JD
  TITLE     Combinatorial engineering of intergenic regions in operons tunes 
            expression of multiple genes.
  JOURNAL   Nat Biotechnol. 2006 Aug . 24(8):1027-32.
  PUBMED    16845378
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2006 Aug . 24(8):1027-32."
COMMENT     Sequence Label: p70rg 15
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(216..234)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(266..286)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     484..503
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     678..701
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gnctactgag cttttttcgc aactctctac tgtttctcca tacgtcgaca gctagcgtat
       61 tttggatgat aacgaggcgc aaaaaatggc gagcagtgag gacatcatca aggagttcat
      121 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      181 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
      241 ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta
      301 cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa
      361 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
      421 cctgcaggac ggctccttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga
      481 cggccccgta atgcagaaga agactatggg ctgggaggcc tccaccgagc gcctgtaccc
      541 ccgcgacggc gtgctgaagg gcgagatcca caaggccctg aagctgaagg acggcggcca
      601 ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta
      661 ctactacgtg gactccaagc tggacatcac ctcccacaac gaggantaca ccatcgtgga
      721 acagtacgag cgcgccgagg gccgccacca cctgtcctnt agatctctga tcacaccnga
      781 aatctcatag ntggncagnt gagatgtgga tcaggcgtga t
//
