LOCUS       sequence-237538-       12694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus delivery for stable expression of LbCas12a-VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Breinig M, Schweitzer AY, Herianto AM, Revia S, Schaefer L, Wendler 
            L, Cobos Galvez A, Tschaharganeh DF
  TITLE     Multiplexed orthogonal genome editing and transcriptional activation
            by Cas12a.
  JOURNAL   Nat Methods. 2019 Jan;16(1):51-54. doi: 10.1038/s41592-018-0262-1. 
            Epub 2018 Dec 17.
  PUBMED    30559432
REFERENCE   2  (bases 1 to 12694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0262-1"; journalName: "Nat Methods"; date: 
            "2019-01"; volume: "16"; issue: "1"; pages: "51-54"
COMMENT     Sequence Label: pMB34
FEATURES             Location/Qualifiers
     source          1..12694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2679..2890
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2933..2942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2945..2965
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2990..6670
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             6671..6718
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    6761..6785
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6815..6964
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6989..7009
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7439..7795
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             7814..8383
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     promoter        8411..8910
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8446..8469)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8843..8862
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8931..9530
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8931..8950)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9427..9447
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9583..10171
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9636..9656)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10054..10065)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10174..10190)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10175..10191)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10700..10880
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10942..11530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11022..11041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11701..12492,12493..12561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12324..12343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12562..12666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgggtcgact agtgatcagt gtgagggagt
     2641 gtaaagctgg ttttcgagtg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2701 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2761 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2821 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2881 cagaacacag gtgtcgtgac gcggctaact cgaacgctag ctgtgcgatc gtgccaccat
     2941 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagcca gcaagctgga
     3001 gaagtttaca aactgctact ccctgtctaa gaccctgagg ttcaaggcca tccctgtggg
     3061 caagacccag gagaacatcg acaataagcg gctgctggtg gaggacgaga agagagccga
     3121 ggattataag ggcgtgaaga agctgctgga tcgctactat ctgtctttta tcaacgacgt
     3181 gctgcacagc atcaagctga agaatctgaa caattacatc agcctgttcc ggaagaaaac
     3241 cagaaccgag aaggagaata aggagctgga gaacctggag atcaatctgc ggaaggagat
     3301 cgccaaggcc ttcaagggca acgagggcta caagtccctg tttaagaagg atatcatcga
     3361 gacaatcctg ccagagttcc tggacgataa ggacgagatc gccctggtga acagcttcaa
     3421 tggctttacc acagccttca ccggcttctt tgataacaga gagaatatgt tttccgagga
     3481 ggccaagagc acatccatcg ccttcaggtg tatcaacgag aatctgaccc gctacatctc
     3541 taatatggac atcttcgaga aggtggacgc catctttgat aagcacgagg tgcaggagat
     3601 caaggagaag atcctgaaca gcgactatga tgtggaggat ttctttgagg gcgagttctt
     3661 taactttgtg ctgacacagg agggcatcga cgtgtataac gccatcatcg gcggcttcgt
     3721 gaccgagagc ggcgagaaga tcaagggcct gaacgagtac atcaacctgt ataatcagaa
     3781 aaccaagcag aagctgccta agtttaagcc actgtataag caggtgctga gcgatcggga
     3841 gtctctgagc ttctacggcg agggctatac atccgatgag gaggtgctgg aggtgtttag
     3901 aaacaccctg aacaagaaca gcgagatctt cagctccatc aagaagctgg agaagctgtt
     3961 caagaatttt gacgagtact ctagcgccgg catctttgtg aagaacggcc ccgccatcag
     4021 cacaatctcc aaggatatct tcggcgagtg gaacgtgatc cgggacaagt ggaatgccga
     4081 gtatgacgat atccacctga agaagaaggc cgtggtgacc gagaagtacg aggacgatcg
     4141 gagaaagtcc ttcaagaaga tcggctcctt ttctctggag cagctgcagg agtacgccga
     4201 cgccgatctg tctgtggtgg agaagctgaa ggagatcatc atccagaagg tggatgagat
     4261 ctacaaggtg tatggctcct ctgagaagct gttcgacgcc gattttgtgc tggagaagag
     4321 cctgaagaag aacgacgccg tggtggccat catgaaggac ctgctggatt ctgtgaagag
     4381 cttcgagaat tacatcaagg ccttctttgg cgagggcaag gagacaaaca gggacgagtc
     4441 cttctatggc gattttgtgc tggcctacga catcctgctg aaggtggacc acatctacga
     4501 tgccatccgc aattatgtga cccagaagcc ctactctaag gataagttca agctgtattt
     4561 tcagaaccct cagttcatgg gcggctggga caaggataag gagacagact atcgggccac
     4621 catcctgaga tacggctcca agtactatct ggccatcatg gataagaagt acgccaagtg
     4681 cctgcagaag atcgacaagg acgatgtgaa cggcaattac gagaagatca actataagct
     4741 gctgcccggc cctaataaga tgctgccaaa ggtgttcttt tctaagaagt ggatggccta
     4801 ctataacccc agcgaggaca tccagaagat ctacaagaat ggcacattca agaagggcga
     4861 tatgtttaac ctgaatgact gtcacaagct gatcgacttc tttaaggata gcatctcccg
     4921 gtatccaaag tggtccaatg cctacgattt caacttttct gagacagaga agtataagga
     4981 catcgccggc ttttacagag aggtggagga gcagggctat aaggtgagct tcgagtctgc
     5041 cagcaagaag gaggtggata agctggtgga ggagggcaag ctgtatatgt tccagatcta
     5101 taacaaggac ttttccgata agtctcacgg cacacccaat ctgcacacca tgtacttcaa
     5161 gctgctgttt gacgagaaca atcacggaca gatcaggctg agcggaggag cagagctgtt
     5221 catgaggcgc gcctccctga agaaggagga gctggtggtg cacccagcca actcccctat
     5281 cgccaacaag aatccagata atcccaagaa aaccacaacc ctgtcctacg acgtgtataa
     5341 ggataagagg ttttctgagg accagtacga gctgcacatc ccaatcgcca tcaataagtg
     5401 ccccaagaac atcttcaaga tcaatacaga ggtgcgcgtg ctgctgaagc acgacgataa
     5461 cccctatgtg atcggcatcg ataggggcga gcgcaatctg ctgtatatcg tggtggtgga
     5521 cggcaagggc aacatcgtgg agcagtattc cctgaacgag atcatcaaca acttcaacgg
     5581 catcaggatc aagacagatt accactctct gctggacaag aaggagaagg agaggttcga
     5641 ggcccgccag aactggacct ccatcgagaa tatcaaggag ctgaaggccg gctatatctc
     5701 tcaggtggtg cacaagatct gcgagctggt ggagaagtac gatgccgtga tcgccctgga
     5761 ggacctgaac tctggcttta agaatagccg cgtgaaggtg gagaagcagg tgtatcagaa
     5821 gttcgagaag atgctgatcg ataagctgaa ctacatggtg gacaagaagt ctaatccttg
     5881 tgcaacaggc ggcgccctga agggctatca gatcaccaat aagttcgaga gctttaagtc
     5941 catgtctacc cagaacggct tcatctttta catccctgcc tggctgacat ccaagatcga
     6001 tccatctacc ggctttgtga acctgctgaa aaccaagtat accagcatcg ccgattccaa
     6061 gaagttcatc agctcctttg acaggatcat gtacgtgccc gaggaggatc tgttcgagtt
     6121 tgccctggac tataagaact tctctcgcac agacgccgat tacatcaaga agtggaagct
     6181 gtactcctac ggcaaccgga tcagaatctt ccggaatcct aagaagaaca acgtgttcga
     6241 ctgggaggag gtgtgcctga ccagcgccta taaggagctg ttcaacaagt acggcatcaa
     6301 ttatcagcag ggcgatatca gagccctgct gtgcgagcag tccgacaagg ccttctactc
     6361 tagctttatg gccctgatga gcctgatgct gcagatgcgg aacagcatca caggccgcac
     6421 cgacgtggat tttctgatca gccctgtgaa gaactccgac ggcatcttct acgatagccg
     6481 gaactatgag gcccaggaga atgccatcct gccaaagaac gccgacgcca atggcgccta
     6541 taacatcgcc agaaaggtgc tgtgggccat cggccagttc aagaaggccg aggacgagaa
     6601 gctggataag gtgaagatcg ccatctctaa caaggagtgg ctggagtacg cccagaccag
     6661 cgtgaagcac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg
     6721 atcctcgcca gggatccgtc gacttgacgc gttaatatca acaagtttgt acaaaaaagc
     6781 aggctacaaa gaggccagcg gttccggacg ggctgacgca ttggacgatt ttgatctgga
     6841 tatgctggga agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct
     6901 tgatgacttt gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat
     6961 gctgattaac tctagaagtt ccggatctcc gaaaaagaaa cgcaaagttg gtagccagta
     7021 cctgcccgac accgacgacc ggcaccggat cgaggaaaag cggaagcgga cctacgagac
     7081 attcaagagc atcatgaaga agtccccctt cagcggcccc accgacccta gacctccacc
     7141 tagaagaatc gccgtgccca gcagatccag cgccagcgtg ccaaaacctg ccccccagcc
     7201 ttaccccttc accagcagcc tgagcaccat caactacgac gagttcccta ccatggtgtt
     7261 ccccagcggc cagatctctc aggcctctgc tctggctcca gcccctcctc aggtgctgcc
     7321 tcaggctcct gctcctgcac cagctccagc catggtgtct gcactggctc aggcaccagc
     7381 acccgtgcct gtgctggctc ctggacctcc acaggctgtg gctccaccag cccctaaacc
     7441 tacacaggcc ggcgagggca cactgtctga agctctgctg cagctgcagt tcgacgacga
     7501 ggatctggga gccctgctgg gaaacagcac cgatcctgcc gtgttcaccg acctggccag
     7561 cgtggacaac agcgagttcc agcagctgct gaaccagggc atccctgtgg cccctcacac
     7621 caccgagccc atgctgatgg aataccccga ggccatcacc cggctcgtga caggcgctca
     7681 gaggcctcct gatccagctc ctgcccctct gggagcacca ggcctgccta atggactgct
     7741 gtctggcgac gaggacttca gctctatcgc cgatatggat ttctcagcct tgctgggctc
     7801 tggcagcggc agccgggatt ccagggaagg gatgtttttg ccgaagcctg aggccggctc
     7861 cgctattagt gacgtgtttg agggccgcga ggtgtgccag ccaaaacgaa tccggccatt
     7921 tcatcctcca ggaagtccat gggccaaccg cccactcccc gccagcctcg caccaacacc
     7981 aaccggtcca gtacatgagc cagtcgggtc actgaccccg gcaccagtcc ctcagccact
     8041 ggatccagcg cccgcagtga ctcccgaggc cagtcacctg ttggaggatc ccgatgaaga
     8101 aaccagccag gctgtcaaag cccttcggga gatggccgat actgtgattc cccagaagga
     8161 agaggctgca atctgtggcc aaatggacct ttcccatccg cccccaaggg gccatctgga
     8221 tgagctgaca accacacttg agtccatgac cgaggatctg aacctggact cacccctgac
     8281 cccggaattg aacgagattc tggatacctt cctgaacgac gagtgcctct tgcatgccat
     8341 gcatatcagc acaggactgt ccatcttcga cacatctctg ttttgaccta ggaatcccga
     8401 ggcctctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     8461 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     8521 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     8581 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     8641 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     8701 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     8761 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     8821 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     8881 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
     8941 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     9001 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     9061 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     9121 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     9181 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     9241 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     9301 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     9361 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     9421 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     9481 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga aaacgttgtt
     9541 gtttggggtt gaattactct tctagaccgc ggcgcgcccg ataatcaacc tctggattac
     9601 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9661 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9721 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9781 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9841 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9901 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9961 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    10021 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    10081 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    10141 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
    10201 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
    10261 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
    10321 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
    10381 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
    10441 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
    10501 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
    10561 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
    10621 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
    10681 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10741 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10801 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10861 agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    10921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11341 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    11401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    11461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    11521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    11641 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    11701 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    11761 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    11821 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    11881 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11941 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12001 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12061 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12121 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12181 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12241 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12301 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    12361 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    12421 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    12481 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    12541 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    12601 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    12661 tccgcgcaca tttccccgaa aagtgccacc tgac
//
