LOCUS       sequence-237542-         601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-100-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-100-5p
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(100..124)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(348..367)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    413..468
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(467..486)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ccgacaaaga gacatacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
       61 ctagcaaact ggggcacaaa cttaattgag aattctacca cccagctttc ttgtacaaag
      121 tggtacgtct caagaccaga ggtctctgct ttcgtagggc acaagttcgg atctacgggt
      181 tcacaagttc ggatctacgg gttcacaagt tcggatctac gggttcacaa gttcggatct
      241 acgggttcaa ccagaagaga cctgtgcttc actcctcagg tgcaggctgc ctatcagaag
      301 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
      361 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
      421 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
      481 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
      541 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
      601 a
//
