LOCUS       sequence-237544-        5691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse ID1-eGFP for HR to create reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Huang YH, Hu J, Chen F, Lecomte N, Basnet H, David CJ, Witkin MD, 
            Allen PJ, Leach SD, Hollmann TJ, Iacobuzio-Donahue CA, Massague J
  TITLE     ID1 mediates escape from TGF-beta tumor suppression in pancreatic 
            cancer.
  JOURNAL   Cancer Discov. 2019 Oct 3. pii: 2159-8290.CD-19-0529. doi: 
            10.1158/2159-8290.CD-19-0529.
  PUBMED    31582374
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2019 Oct 3. pii: 2159-8290.CD-19-0529. doi: 
            10.1158/2159-8290.CD-19-0529."
COMMENT     Sequence Label: ID1GFP Donor
FEATURES             Location/Qualifiers
     source          1..5691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1093..1095,1096..1809)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1093..1095,1096..1806)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1135..1156)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1396..1415)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1743..1764
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2970..2986)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2970..2986)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2983..3005)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2994..3010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3018..3024,3025..3042,3043..3048))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3063..3084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3201..3218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3372..3960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3452..3471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4131..4922,4923..4991))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4754..4773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4992..5096)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5164..5182
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5220..5242)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5342..5361
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5555..5577
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5569..5586
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5570..5586
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtttaatct gcaaaatgga cctggagaag tgagaaagag accgtgagaa gctgattcct
       61 ggggtctttt tgttaaccgc aaaattagct tagtctcttt gcttccttga ggaagagccc
      121 actagctgct ctacctatgc cctcccacac tttcagttct tgagccccca tcacctctcc
      181 atcagcctaa gggtgtacta gtagatccag aggtgggacc cagaggcgct caaagccacg
      241 cctctagcag cctattggtt gcttttgaac gttctgaacc cgcccttggt ctgcccttat
      301 aaaagactgg ctccagcgct ctgccctctc attgtacaac ctttctccaa cttcttgttc
      361 tcttcccaca ctctgttctc agcctcctcc gctcccctcc gcctgttctc aggatcatga
      421 aggtcgccag tggcagtgcc gcagccgctg caggccctag ctgttcgctg aaggcgggca
      481 ggacagcggg cgaggtggta ctgggtctgt cggagcaaag cgtggccatc tcgcgctgcg
      541 ctgggacgcg cctgcccgcc ttgctagacg agcagcaggt gaacgtcctg ctctacgaca
      601 tgaacggctg ctactcacgc ctcaaggagc tggtgcccac cctgccccag aaccgcaaag
      661 tgagcaaggt ggagatcctg cagcatgtaa tcgactacat cagggacctg cagctggagc
      721 tgaactcgga gtctgaagtc gggaccaccg gaggccgggg actgcctgtc cgcgccccgc
      781 tcagcaccct gaacggcgag atcagtgcct tggcggccga ggtgaggtcc gagtcagagt
      841 attacattat tcttcagtgg gaaactgagg ccacgggagg cgggtgtccc ccttccttac
      901 ttttcaggcg catagctatt taggggcgac taataggaaa aagctcgcat tttcatcgtg
      961 cctcctggag tagagaaatg ggaacgcctc tcccctcctt gtcctttcca gtgggtctca
     1021 tcccttatct cgctctggtg ttcacaggcg gcatgtgttc cagccgacga tcgcatcttg
     1081 tgtcgcggta ccgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1141 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1201 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1261 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1321 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1381 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1441 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1501 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1561 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1621 cagctcgccg atcactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1681 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1741 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1801 tacaagtagg gatccggcgg cgcactgagg gaccagatgg actccagccc ttcaggaggc
     1861 aagaggaaaa aagtgctctc ggttccccag ggatctctgg gaaagacact accgcagcca
     1921 ccggactctt ggcggatcgg tccagtgggt agagggtttg atcaacagag cctcaccctc
     1981 tccacctttc agcctccaga gactttgggg agggggttaa tcaaccccgc gtgtttctgt
     2041 tttattgaaa aagcagacat tttttttaaa tggtcacatt tcgtgcttct cggatttctg
     2101 aggaaatatt ttgtattgta tattacaatg atcactggct gaaaatattg ttttacaata
     2161 gttctatggg ggtgggtttt ttgttgttat taaacaaaca ctttagataa cgttgctgtg
     2221 acttcttgga tgggggaggg gcgggacagg catgacctct ggccattttc acagctggtg
     2281 ttgagaatct gggggattgg atggccacat ctgggctgtc attcttaccg acctgaatga
     2341 gtgtttccgg tgtctttaat gtctattctt tccctcatct ctggccattt ccctgttccc
     2401 tggtgtcata aaatcaaatc ctctagacct ctagtagggc tttctctcaa cgtggtccct
     2461 aggccatctg gatcacccag ggaggttgtt aaaatgcaga tggacctgct aagtgtcctg
     2521 ctctgggctg ggaagtattc aagccattca agatgggaaa gcgggtcact ggaccattca
     2581 tttccttacc aaggacaggg gtttgtgtgt gagacctagt gaaaagggct tgcctaaggt
     2641 cagagctggc ctgaagcagg acttctgttg ccccctcttc ccggctgagc cacctttctc
     2701 tggaggccag caggctgccc taggtctcct gccttgtcca agtgggtgat gaaattggca
     2761 gggttaggct gtacacagct gcagggccac ctgctctagg tctggactca gccccctcac
     2821 cctggcctgg ctttgctggc agatcaacac aacacccccc tccgccccag gctactgtgt
     2881 gggcgagtca tgtccccaac tcctgagaag gaaaaaggtt ttccttaggt tttgtctacc
     2941 tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     3001 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     3061 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3121 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3181 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3241 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3301 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3361 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3421 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3481 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3541 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3601 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3661 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3721 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3781 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3841 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3901 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3961 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4021 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4081 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4141 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4201 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4261 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4321 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4381 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4441 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4501 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4561 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4621 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4681 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4741 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4801 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4861 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4921 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4981 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5041 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5101 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     5161 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     5221 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5281 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     5341 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     5401 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     5461 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     5521 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     5581 gccagtgaat tcaataagta gagatcacag cccaatcttc caacagcatc tgggaatcct
     5641 tgacttgtcc tgagtcactg gccaacccaa agctagccac ttccccgttc c
//
